##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632738.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	18924
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.01875924751638	34.0	33.0	34.0	31.0	34.0
2	33.176812513210734	34.0	33.0	34.0	31.0	34.0
3	33.25951173113506	34.0	34.0	34.0	31.0	34.0
4	36.529169308814204	37.0	37.0	37.0	35.0	37.0
5	36.50644683999155	37.0	37.0	37.0	35.0	37.0
6	36.56230183893469	37.0	37.0	37.0	35.0	37.0
7	36.55701754385965	37.0	37.0	37.0	35.0	37.0
8	36.53165292749947	37.0	37.0	37.0	35.0	37.0
9	38.32080955400549	39.0	39.0	39.0	37.0	39.0
10	38.372489959839356	39.0	39.0	39.0	37.0	39.0
11	38.41883322764743	39.0	39.0	39.0	37.0	39.0
12	38.41249207355739	39.0	39.0	39.0	37.0	39.0
13	38.41032551257662	39.0	39.0	39.0	37.0	39.0
14	39.996565208201226	41.0	40.0	41.0	38.0	41.0
15	39.97305009511731	41.0	40.0	41.0	38.0	41.0
16	39.91989008666244	41.0	40.0	41.0	38.0	41.0
17	39.94414500105686	41.0	40.0	41.0	38.0	41.0
18	39.936694145001056	41.0	40.0	41.0	38.0	41.0
19	39.92311350665821	41.0	40.0	41.0	38.0	41.0
20	39.90282181357007	41.0	40.0	41.0	38.0	41.0
21	39.87729866835764	41.0	40.0	41.0	38.0	41.0
22	39.84839357429719	41.0	40.0	41.0	38.0	41.0
23	39.78778270978651	41.0	40.0	41.0	38.0	41.0
24	39.78107165504122	41.0	40.0	41.0	38.0	41.0
25	39.714436694145	41.0	40.0	41.0	38.0	41.0
26	39.643891354893256	41.0	40.0	41.0	37.0	41.0
27	39.589093214965125	41.0	40.0	41.0	37.0	41.0
28	39.52092580849715	41.0	40.0	41.0	37.0	41.0
29	39.496935108856476	41.0	40.0	41.0	37.0	41.0
30	39.456563094483194	41.0	39.0	41.0	37.0	41.0
31	39.39146057915874	41.0	39.0	41.0	36.0	41.0
32	39.35896216444726	41.0	39.0	41.0	36.0	41.0
33	39.28302684421898	41.0	39.0	41.0	36.0	41.0
34	39.23911435214542	41.0	39.0	41.0	36.0	41.0
35	39.16391883322765	41.0	39.0	41.0	35.0	41.0
36	39.09353202282816	41.0	39.0	41.0	35.0	41.0
37	39.028059606848444	41.0	39.0	41.0	35.0	41.0
38	38.97812301838935	41.0	39.0	41.0	35.0	41.0
39	38.89119636440499	40.0	39.0	41.0	35.0	41.0
40	38.80432255337138	40.0	38.0	41.0	35.0	41.0
41	38.7498943140985	40.0	38.0	41.0	35.0	41.0
42	38.68986472204608	40.0	38.0	41.0	35.0	41.0
43	37.98446417247939	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	0.0
19	1.0
20	1.0
21	5.0
22	5.0
23	4.0
24	13.0
25	22.0
26	28.0
27	36.0
28	45.0
29	58.0
30	78.0
31	96.0
32	129.0
33	150.0
34	253.0
35	443.0
36	754.0
37	1506.0
38	3783.0
39	11513.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.07355738744452	16.788205453392518	12.35996618051152	28.77827097865145
2	21.322130627774253	18.864933417882053	31.51553582752061	28.29740012682308
3	20.835975480870854	19.562460367786937	28.625026421475376	30.976537729866838
4	15.778905094060452	15.197632635806382	33.528852251109704	35.49461001902346
5	17.744662861974213	33.09025575988163	32.22891566265061	16.936165715493555
6	35.83280490382583	32.96343267808074	14.833016275628829	16.370746142464597
7	31.51553582752061	27.27753117734094	18.849080532656945	22.357852462481507
8	27.467765800042276	30.77573451701543	19.55189177763686	22.204607905305433
9	27.779539209469455	12.645318114563517	17.51743817374762	42.05770450221941
10	19.54132318748679	22.94440921581061	29.549778059606847	27.964489537095748
11	39.6005072923272	19.197844007609387	18.463326992179244	22.738321707884168
12	23.24561403508772	23.567956034664974	25.9141830479814	27.272246882265904
13	34.569858380891986	17.369477911646587	20.656309448319593	27.404354259141833
14	24.783343901923484	18.637708729655465	22.415979708306914	34.16296766011414
15	29.08476009300359	23.552103149439866	19.85309659691397	27.510040160642568
16	26.23124075248362	23.50454449376453	21.83999154512788	28.42422320862397
17	27.377932783766646	23.287888395688014	20.830691185795818	28.503487634749526
18	26.66455294863665	21.163601775523144	23.33544705136335	28.836398224476856
19	28.582752060875077	21.739589938702178	22.833439019234834	26.84421898118791
20	30.00951173113507	20.59289790741915	22.357852462481507	27.039737898964276
21	28.181145635172268	22.00908898752906	21.374973578524624	28.434791798774047
22	28.540477700274785	22.29444092158106	20.175438596491226	28.98964278165293
23	28.852251109701964	22.151764954555063	21.512365250475586	27.483618685267388
24	28.846966814626928	21.903403086028327	20.92580849714648	28.32382160219827
25	29.290847600930036	21.54407102092581	21.729021348552102	27.436060029592053
26	28.852251109701964	22.220460790530545	21.42253223419996	27.50475586556753
27	27.805960684844642	21.834707250052844	22.828154724159795	27.53117734094272
28	27.134855210314946	22.896850560135277	21.285140562248998	28.683153667300783
29	28.32382160219827	23.08708518283661	21.43310082435003	27.15599239061509
30	27.219403931515533	22.08835341365462	23.40414288733883	27.288099767491016
31	28.03846966814627	21.929824561403507	21.602198266751216	28.429507503699007
32	26.188966391883323	22.072500528429508	22.47410695413232	29.26442612555485
33	26.66455294863665	21.411963644049884	24.35003170577045	27.57345170154301
34	27.023885013739168	21.40139505389981	23.462270133164235	28.11244979919679
35	26.257662227858802	22.34728387233143	24.693510885647854	26.70154301416191
36	26.40562248995984	21.33269921792433	24.852039737898966	27.40963855421687
37	26.902346227013318	21.385542168674696	23.8427393785669	27.86937222574509
38	25.52314521242866	20.217712957091525	25.877192982456144	28.381948848023676
39	25.396322130627773	19.446205876136126	26.828366095962796	28.329105897273305
40	24.899598393574294	20.291693088142043	27.81124497991968	26.99746353836398
41	23.573240329740013	19.483195941661382	28.783555273726485	28.16000845487212
42	21.950961741703658	21.03677869372226	30.004227436060027	27.00803212851406
43	21.533502430775734	20.793701120270555	28.979074191502853	28.693722257450855
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	1.0
2	0.0
3	0.5
4	1.0
5	1.0
6	1.0
7	1.0
8	2.0
9	3.0
10	3.0
11	3.0
12	3.0
13	5.0
14	7.0
15	13.5
16	20.0
17	17.5
18	15.0
19	15.0
20	17.5
21	20.0
22	17.5
23	15.0
24	20.0
25	25.0
26	25.0
27	28.5
28	32.0
29	56.0
30	80.0
31	97.0
32	114.0
33	114.0
34	155.0
35	196.0
36	217.5
37	239.0
38	374.0
39	509.0
40	509.0
41	553.0
42	597.0
43	700.0
44	803.0
45	995.5
46	1188.0
47	1188.0
48	1370.0
49	1552.0
50	1714.0
51	1876.0
52	1981.0
53	2086.0
54	2086.0
55	2032.5
56	1979.0
57	1870.0
58	1761.0
59	1652.5
60	1544.0
61	1544.0
62	1439.5
63	1335.0
64	1134.5
65	934.0
66	810.0
67	686.0
68	686.0
69	565.5
70	445.0
71	404.5
72	364.0
73	285.0
74	206.0
75	206.0
76	173.5
77	141.0
78	101.0
79	61.0
80	48.0
81	35.0
82	35.0
83	28.5
84	22.0
85	16.0
86	10.0
87	9.0
88	8.0
89	8.0
90	8.0
91	8.0
92	4.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	18924.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.50348763474952
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.31071620893914	70.11202705559079
2	5.9302638664512655	9.310927922215177
3	2.0328486806677435	4.787571337983513
4	0.78082929456112	2.4519129148171634
5	0.5654281098546041	2.219403931515536
6	0.32310177705977383	1.5218769816106532
7	0.30963920301561654	1.7015430141619108
8	0.12116316639741519	0.7609384908053266
9	0.17501346257404415	1.2365250475586558
>10	0.44426494345718903	5.506235468188544
>50	0.006731287022078622	0.39103783555273725
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	74	0.39103783555273725	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	42	0.22194039315155356	No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT	42	0.22194039315155356	TruSeq Adapter, Index 1 (95% over 21bp)
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	40	0.2113718030014796	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	25	0.13210737687592475	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	24	0.12682308180088775	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	24	0.12682308180088775	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	24	0.12682308180088775	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	23	0.12153878672585076	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	23	0.12153878672585076	No Hit
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC	22	0.11625449165081378	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	22	0.11625449165081378	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	21	0.11097019657577678	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	21	0.11097019657577678	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	20	0.1056859015007398	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	19	0.1004016064257028	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	19	0.1004016064257028	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.00528429507503699	0.0
23	0.0	0.0	0.0	0.00528429507503699	0.0
24	0.0	0.0	0.0	0.00528429507503699	0.0
25	0.0	0.0	0.0	0.00528429507503699	0.0
26	0.0	0.0	0.0	0.02113718030014796	0.0
27	0.0	0.0	0.0	0.06341154090044387	0.0
28	0.0	0.0	0.0	0.16381314732614669	0.0
29	0.0	0.0	0.0	0.31705770450221943	0.0
30	0.0	0.0	0.0	0.54428239272881	0.0
31	0.0	0.0	0.0	1.0938490805326568	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTCTTA	105	8.411156E-6	15.857142	37
GTCTCTT	160	2.3205394E-6	12.71875	37
CCTGTCT	135	8.998784E-5	12.333334	34
TGTCTCT	180	8.6612035E-6	11.305555	36
CTGTCTC	205	3.6565863E-5	9.92683	35
>>END_MODULE