FastQCFastQC Report
Fri 10 Feb 2017
ERR1632737.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632737.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23945
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT730.30486531634996866Illumina PCR Primer Index 11 (95% over 22bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA630.26310294424723324No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA420.17540196283148882No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT410.1712257256212153No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA390.1628732512006682No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA380.15869701399039465No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA370.1545207767801211No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC370.1545207767801211No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA370.1545207767801211No Hit
CATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTC340.1419920651493005No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA340.1419920651493005No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT340.1419920651493005No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT330.13781582793902694No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC330.13781582793902694No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA320.1336395907287534No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG320.1336395907287534No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC310.12946335351847985No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA300.1252871163082063No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC290.12111087909793276No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA280.11693464188765922No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC270.11275840467738568No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT250.10440593025683859No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA250.10440593025683859No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA240.10022969304656504No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC240.10022969304656504No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC240.10022969304656504No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA200.00180611437.02
GGTATCA250.005390662229.61
AACGCAG554.943534E-420.1818185
ACGCAGA554.943534E-420.1818186
TCAACGC554.943534E-420.1818183
TATCAAC554.943534E-420.1818181
ATCAACG554.943534E-420.1818182
CAACGCA554.943534E-420.1818184
CAGAGTA608.881285E-418.59
AGTACGG608.881285E-418.512
AGAGTAC608.881285E-418.510
GAGTACG608.881285E-418.511
GCAGAGT608.881285E-418.58
CGCAGAG608.881285E-418.57
GTACGGG750.00395021814.813
CTCTTAT850.00905716513.05882437
TGTCTCT2104.1655603E-610.57142937
CTGTCTC2503.3078002E-58.8836
Produced by FastQC (version 0.11.5)