##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632737.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23945
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.024639799540616	34.0	33.0	34.0	31.0	34.0
2	33.15802881603675	34.0	33.0	34.0	31.0	34.0
3	33.239173105032364	34.0	34.0	34.0	31.0	34.0
4	36.52963040300689	37.0	37.0	37.0	35.0	37.0
5	36.48807684276467	37.0	37.0	37.0	35.0	37.0
6	36.537314679473795	37.0	37.0	37.0	35.0	37.0
7	36.52967216537899	37.0	37.0	37.0	35.0	37.0
8	36.511630820630614	37.0	37.0	37.0	35.0	37.0
9	38.28874504071831	39.0	39.0	39.0	37.0	39.0
10	38.337022342869076	39.0	39.0	39.0	37.0	39.0
11	38.40785132595531	39.0	39.0	39.0	37.0	39.0
12	38.36191271664231	39.0	39.0	39.0	37.0	39.0
13	38.41006473167676	39.0	39.0	39.0	37.0	39.0
14	39.96061808310712	41.0	40.0	41.0	38.0	41.0
15	39.94683650031322	41.0	40.0	41.0	38.0	41.0
16	39.8961787429526	41.0	40.0	41.0	38.0	41.0
17	39.89751513885989	41.0	40.0	41.0	38.0	41.0
18	39.892754228440175	41.0	40.0	41.0	38.0	41.0
19	39.91664230528294	41.0	40.0	41.0	38.0	41.0
20	39.87199832950512	41.0	40.0	41.0	38.0	41.0
21	39.825140948005846	41.0	40.0	41.0	38.0	41.0
22	39.81135936521194	41.0	40.0	41.0	38.0	41.0
23	39.7435372729171	41.0	40.0	41.0	38.0	41.0
24	39.75021925245354	41.0	40.0	41.0	38.0	41.0
25	39.71351012737524	41.0	40.0	41.0	38.0	41.0
26	39.617122572562124	41.0	40.0	41.0	37.0	41.0
27	39.555815410315304	41.0	40.0	41.0	37.0	41.0
28	39.484694090624345	41.0	40.0	41.0	37.0	41.0
29	39.44030068907914	41.0	40.0	41.0	37.0	41.0
30	39.36500313217791	41.0	39.0	41.0	36.0	41.0
31	39.31555648360827	41.0	39.0	41.0	36.0	41.0
32	39.27032783462101	41.0	39.0	41.0	36.0	41.0
33	39.2015034453957	41.0	39.0	41.0	35.0	41.0
34	39.175026101482565	41.0	39.0	41.0	35.0	41.0
35	39.06385466694508	41.0	39.0	41.0	35.0	41.0
36	39.01657966172478	41.0	39.0	41.0	35.0	41.0
37	38.9709751513886	41.0	39.0	41.0	35.0	41.0
38	38.879432031739405	40.0	39.0	41.0	35.0	41.0
39	38.78697013990395	40.0	38.0	41.0	35.0	41.0
40	38.71405303821257	40.0	38.0	41.0	35.0	41.0
41	38.65199415326791	40.0	38.0	41.0	35.0	41.0
42	38.61407391939862	40.0	38.0	41.0	35.0	41.0
43	37.88469409062435	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	4.0
23	12.0
24	17.0
25	19.0
26	32.0
27	39.0
28	61.0
29	97.0
30	112.0
31	150.0
32	200.0
33	254.0
34	381.0
35	598.0
36	940.0
37	1874.0
38	4653.0
39	14501.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.84819377740656	16.015869701399037	12.783462100647316	28.352474420547086
2	21.315514721236166	19.72019210691167	31.083733556065983	27.880559615786176
3	21.67467112131969	19.014408018375445	28.619753602004593	30.691167258300272
4	16.274796408436	14.917519315097097	32.979745249530175	35.827939026936725
5	17.94946752975569	32.599707663395286	32.457715598245976	16.993109208603048
6	35.01774900814366	32.70828983086239	15.14303612445187	17.130925036542074
7	30.62852370014617	26.702860722489035	19.908122781373983	22.76049279599081
8	27.70933389016496	30.04802672791815	20.488619753602006	21.75401962831489
9	27.475464606389643	12.6122363750261	17.45249530173314	42.45980371685112
10	19.590728753393194	23.295051158905824	28.715807057840887	28.398413029860098
11	39.65754854875757	18.83482981833368	18.38379619962414	23.123825433284612
12	23.462100647316767	23.140530382125704	25.654625182710376	27.74274378784715
13	35.619127166423056	16.759239924827728	20.23386928377532	27.3877636249739
14	24.756734182501567	19.38609313008979	21.76654833994571	34.090624347462935
15	29.32553769054082	23.82543328461057	19.219043641678848	27.629985383169764
16	27.01607851325955	23.315932344957194	21.06076425140948	28.60722489037377
17	27.72186260179578	23.553977865942784	20.7099603257465	28.014199206514927
18	27.266652745875962	21.219461265399875	22.092294842347044	29.421591146377114
19	28.61557736479432	22.472332428481938	22.489037377323033	26.42305282940071
20	30.08978910002088	20.630611818751305	21.99206514930048	27.28753393192733
21	27.3877636249739	21.46168302359574	21.820839423679264	29.329713927751094
22	29.016496136980578	22.01294633535185	20.250574232616415	28.71998329505116
23	28.177072457715596	21.632908749216956	21.620380037586134	28.569638755481314
24	29.333890164961367	21.23616621424097	21.46168302359574	27.968260597201922
25	28.640634788055962	21.465859260806013	22.259344330757987	27.634161620380038
26	28.4610565880142	21.77907705157653	22.238463144706618	27.52140321570265
27	27.60492795990812	21.5786176654834	23.52474420547087	27.291710169137605
28	26.26435581541032	22.68532052620589	21.825015660889537	29.22530799749426
29	27.63833785759031	23.015243265817496	21.56608895385258	27.78032992273961
30	26.8824389225308	22.171643349342244	23.161411568177073	27.784506159949885
31	28.590519941532676	21.315514721236166	21.820839423679264	28.273125913551887
32	26.535811234078093	21.511797870119022	22.1465859260806	29.80580496972228
33	26.301941950302776	21.787429525997076	23.508039256629775	28.402589267070372
34	26.665274587596578	21.561912716642308	22.798078930883275	28.974733764877847
35	26.506577573606183	22.656086865733975	24.639799540613907	26.197536020045938
36	26.81144288995615	21.160993944456045	24.83190645228649	27.195656713301315
37	26.836500313217794	20.664021716433492	24.539569847567343	27.959908122781375
38	25.7924410106494	20.82689496763416	25.62539152223846	27.755272499477968
39	25.21194403842138	19.74107329296304	26.6778032992274	28.36917936938818
40	24.34746293589476	20.68490290248486	27.96408435999165	27.003549801628736
41	22.877427437878474	20.146168302359573	28.78262685320526	28.19377740655669
42	22.079766130716223	20.39256629776571	29.179369388181247	28.348298183336812
43	21.18605136771769	20.973063269993737	29.054082271873042	28.786803090415535
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.0
4	2.0
5	2.0
6	1.5
7	1.0
8	1.5
9	2.0
10	2.0
11	2.0
12	2.0
13	5.0
14	8.0
15	11.5
16	15.0
17	17.0
18	19.0
19	19.0
20	26.0
21	33.0
22	26.0
23	19.0
24	21.0
25	23.0
26	23.0
27	38.0
28	53.0
29	77.5
30	102.0
31	113.0
32	124.0
33	124.0
34	161.0
35	198.0
36	238.0
37	278.0
38	368.5
39	459.0
40	459.0
41	556.0
42	653.0
43	747.0
44	841.0
45	1216.5
46	1592.0
47	1592.0
48	1834.5
49	2077.0
50	2344.5
51	2612.0
52	2815.5
53	3019.0
54	3019.0
55	2739.5
56	2460.0
57	2378.5
58	2297.0
59	2081.0
60	1865.0
61	1865.0
62	1710.5
63	1556.0
64	1344.0
65	1132.0
66	975.0
67	818.0
68	818.0
69	717.0
70	616.0
71	507.0
72	398.0
73	330.5
74	263.0
75	263.0
76	217.5
77	172.0
78	142.5
79	113.0
80	85.5
81	58.0
82	58.0
83	45.5
84	33.0
85	26.5
86	20.0
87	14.0
88	8.0
89	8.0
90	5.0
91	2.0
92	2.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	23945.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.00062643558154
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.91762013729976	64.18041344748382
2	6.24141876430206	9.112549592816872
3	2.2196796338672766	4.861140112758404
4	1.0926773455377574	3.1906452286489877
5	0.5949656750572082	2.1716433493422427
6	0.41762013729977115	1.829191898099812
7	0.2688787185354691	1.3739820421799958
8	0.2402745995423341	1.4032157026519105
9	0.16590389016018306	1.089997911881395
>10	0.8295194508009153	10.219252453539362
>50	0.011441647597254006	0.567968260597202
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT	73	0.30486531634996866	Illumina PCR Primer Index 11 (95% over 22bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	63	0.26310294424723324	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	42	0.17540196283148882	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	41	0.1712257256212153	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	39	0.1628732512006682	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	38	0.15869701399039465	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	37	0.1545207767801211	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	37	0.1545207767801211	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	37	0.1545207767801211	No Hit
CATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTC	34	0.1419920651493005	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	34	0.1419920651493005	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	34	0.1419920651493005	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	33	0.13781582793902694	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	33	0.13781582793902694	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	32	0.1336395907287534	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	32	0.1336395907287534	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	31	0.12946335351847985	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	30	0.1252871163082063	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	29	0.12111087909793276	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	28	0.11693464188765922	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	27	0.11275840467738568	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	25	0.10440593025683859	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	25	0.10440593025683859	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	24	0.10022969304656504	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	24	0.10022969304656504	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	24	0.10022969304656504	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0041762372102735435	0.0
22	0.0	0.0	0.0	0.008352474420547087	0.0
23	0.0	0.0	0.0	0.008352474420547087	0.0
24	0.0	0.0	0.0	0.01252871163082063	0.0
25	0.0	0.0	0.0	0.01252871163082063	0.0
26	0.0	0.0	0.0	0.01252871163082063	0.0
27	0.0	0.0	0.0	0.03758613489246189	0.0
28	0.0	0.0	0.0	0.16704948841094175	0.0
29	0.0	0.0	0.0	0.3006890791396951	0.0
30	0.0	0.0	0.0	0.5136771768636459	0.0
31	0.0	0.0	0.0	1.1401127584046773	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	20	0.001806114	37.0	2
GGTATCA	25	0.0053906622	29.6	1
AACGCAG	55	4.943534E-4	20.181818	5
ACGCAGA	55	4.943534E-4	20.181818	6
TCAACGC	55	4.943534E-4	20.181818	3
TATCAAC	55	4.943534E-4	20.181818	1
ATCAACG	55	4.943534E-4	20.181818	2
CAACGCA	55	4.943534E-4	20.181818	4
CAGAGTA	60	8.881285E-4	18.5	9
AGTACGG	60	8.881285E-4	18.5	12
AGAGTAC	60	8.881285E-4	18.5	10
GAGTACG	60	8.881285E-4	18.5	11
GCAGAGT	60	8.881285E-4	18.5	8
CGCAGAG	60	8.881285E-4	18.5	7
GTACGGG	75	0.003950218	14.8	13
CTCTTAT	85	0.009057165	13.058824	37
TGTCTCT	210	4.1655603E-6	10.571429	37
CTGTCTC	250	3.3078002E-5	8.88	36
>>END_MODULE