FastQCFastQC Report
Fri 10 Feb 2017
ERR1632736.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632736.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences347263
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA3840.11057901360064275No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATTCG303.594352E-430.83333428
GGTATCA2900.029.3448281
TATACAC1300.025.61538537
CGCGCTT400.001928488523.12500212
ATTCGCG400.001928488523.12500230
AATTCGC400.001928488523.12500229
TACTCAG400.001928488523.1250025
GACGCCA400.001928488523.12500214
TTTAGGA502.696843E-422.22
GGCCAAT502.696843E-422.225
AAGTGAG705.0850194E-621.1428597
CTTATAC4750.021.03157837
TTATGAG450.003820514320.5555553
ACTTTTG450.003820514320.55555530
TTCGCGG450.003820514320.55555531
TATTAGA555.1331666E-420.1818182
GTATCAA4250.020.0235272
CTCGTAG656.884417E-519.9230771
AGCGTAC851.2401943E-619.5882361
GTCCAGA851.2401943E-619.5882361
Produced by FastQC (version 0.11.5)