##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632736.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	347263
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.05942182150128	34.0	33.0	34.0	31.0	34.0
2	33.19347295853575	34.0	33.0	34.0	31.0	34.0
3	33.276582878106794	34.0	34.0	34.0	31.0	34.0
4	36.55884156964606	37.0	37.0	37.0	35.0	37.0
5	36.53049129910183	37.0	37.0	37.0	35.0	37.0
6	36.5676619737778	37.0	37.0	37.0	35.0	37.0
7	36.55972274616069	37.0	37.0	37.0	35.0	37.0
8	36.534373083225105	37.0	37.0	37.0	35.0	37.0
9	38.3285319772046	39.0	39.0	39.0	37.0	39.0
10	38.36249758828323	39.0	39.0	39.0	37.0	39.0
11	38.43041153246963	39.0	39.0	39.0	37.0	39.0
12	38.40380057766016	39.0	39.0	39.0	37.0	39.0
13	38.430558395222064	39.0	39.0	39.0	37.0	39.0
14	40.00301212625589	41.0	40.0	41.0	38.0	41.0
15	39.98192724246464	41.0	40.0	41.0	38.0	41.0
16	39.957064242375374	41.0	40.0	41.0	38.0	41.0
17	39.9595090752542	41.0	40.0	41.0	38.0	41.0
18	39.9427839994471	41.0	40.0	41.0	38.0	41.0
19	39.95417306191561	41.0	40.0	41.0	38.0	41.0
20	39.912893109833185	41.0	40.0	41.0	38.0	41.0
21	39.88952753388642	41.0	40.0	41.0	38.0	41.0
22	39.86477108128421	41.0	40.0	41.0	38.0	41.0
23	39.82707630815837	41.0	40.0	41.0	38.0	41.0
24	39.8093923049677	41.0	40.0	41.0	38.0	41.0
25	39.76201610882818	41.0	40.0	41.0	38.0	41.0
26	39.68780434425781	41.0	40.0	41.0	37.0	41.0
27	39.62386433337269	41.0	40.0	41.0	37.0	41.0
28	39.57598419641597	41.0	40.0	41.0	37.0	41.0
29	39.535677570026174	41.0	40.0	41.0	37.0	41.0
30	39.4860811546292	41.0	40.0	41.0	37.0	41.0
31	39.43975315538943	41.0	39.0	41.0	36.0	41.0
32	39.38670690514106	41.0	39.0	41.0	36.0	41.0
33	39.32529235766552	41.0	39.0	41.0	36.0	41.0
34	39.2812997641557	41.0	39.0	41.0	36.0	41.0
35	39.21041400897879	41.0	39.0	41.0	35.0	41.0
36	39.164448847127396	41.0	39.0	41.0	35.0	41.0
37	39.124257407210095	41.0	39.0	41.0	35.0	41.0
38	39.050958495434294	41.0	39.0	41.0	35.0	41.0
39	38.99511321390416	41.0	39.0	41.0	35.0	41.0
40	38.89927518912179	40.0	39.0	41.0	35.0	41.0
41	38.85692112318329	40.0	39.0	41.0	35.0	41.0
42	38.78046610206098	40.0	38.0	41.0	35.0	41.0
43	38.05900715020028	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	0.0
14	1.0
15	0.0
16	1.0
17	1.0
18	9.0
19	6.0
20	23.0
21	21.0
22	58.0
23	129.0
24	197.0
25	267.0
26	410.0
27	536.0
28	740.0
29	970.0
30	1492.0
31	1890.0
32	2522.0
33	3316.0
34	5071.0
35	8485.0
36	13205.0
37	24977.0
38	64286.0
39	218648.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.39938317643976	16.197233796862896	12.218117104327268	28.18526592237008
2	20.26446814086154	18.566619536201667	33.614868269870385	27.554044053066406
3	20.496568882950385	19.62028779340154	28.517866861715763	31.36527646193231
4	15.553917347946658	15.117648583350372	32.57185476137682	36.75657930732615
5	17.301872068144316	33.19904510414297	32.690784794233764	16.80829803347895
6	36.529949922681084	32.79848414602189	14.702401349985458	15.96916458131157
7	31.872960839479013	27.60760576277922	19.355646872831255	21.163786524910513
8	28.025156725594147	31.99505850032972	19.059905604685788	20.919879169390345
9	28.0683516527819	13.361054877715162	17.072363021686733	41.49823044781621
10	19.050402720704483	24.12407886817772	29.962593193055408	26.86292521806239
11	38.890408710401054	19.730578840820932	19.417559601800363	21.96145284697765
12	23.80270860990085	23.041326026671427	26.70454381837397	26.451421545053748
13	33.711336940589696	17.433760579157585	21.609846139669354	27.24505634058336
14	24.219395674172027	18.858041311628362	23.13491503557822	33.78764797862139
15	28.51613906462825	24.292251118028698	20.20716287079245	26.984446946550598
16	26.38144576300959	23.680322982868894	21.59141630406925	28.34681495005227
17	26.55652920121061	23.660453316362524	22.45531484782427	27.32770263460259
18	26.778263160774397	21.940143349565027	23.355497130417007	27.92609635924357
19	28.333856471895942	22.283974969979525	22.727154922925852	26.65501363519868
20	28.42744548080273	22.000328281446627	22.76804612066359	26.80418011708705
21	27.91198601636224	22.480079939411915	21.99053743128407	27.617396612941775
22	28.153013710069892	23.0629234902653	21.36939437832421	27.4146684213406
23	27.988009088212678	22.781580531182417	22.023941508309264	27.206468872295638
24	27.925232460699817	22.13855204844743	22.161877309128815	27.77433818172394
25	28.00384722818152	22.086142203459623	22.773229511926118	27.136781056432735
26	27.7936319158678	22.627518624212772	22.626366759487766	26.952482700431663
27	27.677869511004626	22.3574063461987	22.941401761777097	27.02332238101957
28	26.92282218376274	22.84205342924527	22.587779291200043	27.647345095791948
29	27.36859383234033	23.059755862271537	22.446675862386723	27.124974443001413
30	26.85975759006862	22.80778545367632	23.173502503865944	27.15895445238911
31	27.045495776975955	22.269864627098194	22.677336773569312	28.007302822356543
32	25.51236382799204	22.536521310937243	23.443326815698764	28.507788045371953
33	26.35725660378445	22.006951503615415	24.310105021266303	27.325686871333833
34	26.451421545053748	21.657072593394634	24.29426688129746	27.597238980254158
35	26.362152028865733	22.194417487610256	24.611605613036804	26.83182487048721
36	26.162015532895815	21.404814218618164	24.74291819168757	27.69025205679845
37	25.688311164736817	20.71743894397042	25.028292677307977	28.565957213984788
38	25.0663042132332	20.319469681480605	26.65443770283618	27.959788402450016
39	24.455239976617147	19.579396595663805	27.723944100004893	28.24141932771415
40	23.916455251495265	20.10637470735437	28.856803057048978	27.120366984101384
41	22.905405989120638	19.517195900513443	29.694496678310102	27.882901432055817
42	21.913650460889873	20.147841837454607	30.213987669288116	27.7245200323674
43	21.1704097470793	20.161088281792185	30.32255091962	28.34595105150851
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	10.0
1	6.5
2	3.0
3	9.5
4	16.0
5	16.0
6	32.0
7	48.0
8	41.5
9	35.0
10	46.0
11	57.0
12	57.0
13	118.5
14	180.0
15	305.5
16	431.0
17	451.5
18	472.0
19	472.0
20	554.5
21	637.0
22	645.5
23	654.0
24	831.0
25	1008.0
26	1008.0
27	1222.0
28	1436.0
29	1924.0
30	2412.0
31	3021.5
32	3631.0
33	3631.0
34	4566.5
35	5502.0
36	6457.0
37	7412.0
38	8869.0
39	10326.0
40	10326.0
41	12070.0
42	13814.0
43	15615.5
44	17417.0
45	20321.0
46	23225.0
47	23225.0
48	25549.0
49	27873.0
50	29763.0
51	31653.0
52	33308.0
53	34963.0
54	34963.0
55	33502.5
56	32042.0
57	30172.5
58	28303.0
59	26761.5
60	25220.0
61	25220.0
62	23793.0
63	22366.0
64	19065.0
65	15764.0
66	13866.0
67	11968.0
68	11968.0
69	10470.0
70	8972.0
71	7845.0
72	6718.0
73	5936.5
74	5155.0
75	5155.0
76	4540.5
77	3926.0
78	2888.5
79	1851.0
80	1346.0
81	841.0
82	841.0
83	658.5
84	476.0
85	364.0
86	252.0
87	184.5
88	117.0
89	117.0
90	82.0
91	47.0
92	35.0
93	23.0
94	14.5
95	6.0
96	6.0
97	3.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	347263.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.79566006009227
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.23601764356758	58.05547021932477
2	5.958599231126534	7.840999388910573
3	1.9332032825339382	3.815891580139726
4	0.9685296565786667	2.549001921694715
5	0.6027890353744759	1.9830451229724981
6	0.4077856497663443	1.6098315593646142
7	0.29480584384063896	1.357786155954713
8	0.2082129380036818	1.0959606151202652
9	0.17213223453365123	1.0192998589884175
>10	1.1011794139941657	13.556231432946788
>50	0.08338933620024543	3.700894139141687
>100	0.033355734480065305	3.4155880054412155
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	384	0.11057901360064275	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.879661812516738E-4	0.0
3	0.0	0.0	0.0	2.879661812516738E-4	0.0
4	0.0	0.0	0.0	2.879661812516738E-4	0.0
5	0.0	0.0	0.0	2.879661812516738E-4	0.0
6	2.879661812516738E-4	0.0	0.0	2.879661812516738E-4	0.0
7	2.879661812516738E-4	0.0	0.0	2.879661812516738E-4	0.0
8	2.879661812516738E-4	0.0	0.0	2.879661812516738E-4	0.0
9	2.879661812516738E-4	0.0	0.0	2.879661812516738E-4	0.0
10	2.879661812516738E-4	0.0	0.0	2.879661812516738E-4	0.0
11	2.879661812516738E-4	0.0	0.0	2.879661812516738E-4	0.0
12	2.879661812516738E-4	0.0	0.0	2.879661812516738E-4	0.0
13	2.879661812516738E-4	0.0	0.0	5.759323625033476E-4	0.0
14	2.879661812516738E-4	0.0	0.0	5.759323625033476E-4	0.0
15	5.759323625033476E-4	0.0	0.0	5.759323625033476E-4	0.0
16	8.638985437550214E-4	0.0	0.0	0.0011518647250066951	0.0
17	8.638985437550214E-4	0.0	0.0	0.0023037294500133903	0.0
18	8.638985437550214E-4	0.0	0.0	0.003167627993768412	0.0
19	8.638985437550214E-4	0.0	0.0	0.0040315265375234334	0.0
20	8.638985437550214E-4	0.0	0.0	0.0043194927187751074	0.0
21	8.638985437550214E-4	0.0	0.0	0.006623222168788498	0.0
22	8.638985437550214E-4	0.0	0.0	0.010942714887563605	0.0
23	8.638985437550214E-4	0.0	0.0	0.013822376700080342	0.0
24	8.638985437550214E-4	0.0	0.0	0.016126106150093734	0.0
25	8.638985437550214E-4	0.0	0.0	0.01727797087510043	0.0
26	8.638985437550214E-4	0.0	0.0	0.02102153123137219	0.0
27	8.638985437550214E-4	0.0	0.0	0.047514419906526176	2.879661812516738E-4
28	8.638985437550214E-4	0.0	0.0	0.18746598399483966	2.879661812516738E-4
29	8.638985437550214E-4	0.0	0.0	0.40488045083985336	2.879661812516738E-4
30	8.638985437550214E-4	0.0	0.0	0.6862234099227387	2.879661812516738E-4
31	8.638985437550214E-4	0.0	0.0	1.635935875690759	2.879661812516738E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAATTCG	30	3.594352E-4	30.833334	28
GGTATCA	290	0.0	29.344828	1
TATACAC	130	0.0	25.615385	37
CGCGCTT	40	0.0019284885	23.125002	12
ATTCGCG	40	0.0019284885	23.125002	30
AATTCGC	40	0.0019284885	23.125002	29
TACTCAG	40	0.0019284885	23.125002	5
GACGCCA	40	0.0019284885	23.125002	14
TTTAGGA	50	2.696843E-4	22.2	2
GGCCAAT	50	2.696843E-4	22.2	25
AAGTGAG	70	5.0850194E-6	21.142859	7
CTTATAC	475	0.0	21.031578	37
TTATGAG	45	0.0038205143	20.555555	3
ACTTTTG	45	0.0038205143	20.555555	30
TTCGCGG	45	0.0038205143	20.555555	31
TATTAGA	55	5.1331666E-4	20.181818	2
GTATCAA	425	0.0	20.023527	2
CTCGTAG	65	6.884417E-5	19.923077	1
AGCGTAC	85	1.2401943E-6	19.588236	1
GTCCAGA	85	1.2401943E-6	19.588236	1
>>END_MODULE