FastQCFastQC Report
Fri 10 Feb 2017
ERR1632735.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632735.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1361086
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA24730.18169314797154626No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA22580.16589693818024723No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA21920.16104786912803454No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA21570.1584763931154975No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC20640.15164361399647047No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA18270.1342310478544339No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA17160.12607579535753066No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA17080.12548802941180792No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG16330.1199777236706571No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT16180.11887566252242696No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT15800.11608377428024386No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC14360.1055039872572343No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA13960.10256515752862053No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT13830.10161003786682106No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16550.030.5166171
GTACTAA451.3233871E-424.6666661
ATACGGC1650.023.54545629
GCGGTAA9400.021.45212623
TACACTC450.003826164920.5555555
TATACTG555.143762E-420.1818185
TCTATAC555.143762E-420.1818183
GTCTAGA656.903326E-519.9230771
CGGTAAT10100.019.78217924
AACTCCG10750.019.6186055
CGTGCCA11300.019.48230210
CTTATAC23150.019.33909237
TAGCGAA1457.2759576E-1219.1379310
CGCGGTA10750.019.10232522
AGCCGCG10900.018.83944919
CTAACTC11200.018.8303593
GGTAATA11150.018.74887825
GCTAACT11400.018.5000022
TGAATTA609.2380075E-418.521
TCTATAG609.2380075E-418.53
Produced by FastQC (version 0.11.5)