FastQCFastQC Report
Fri 10 Feb 2017
ERR1632734.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632734.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences233911
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA6330.2706157470148903No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA5120.21888667057128564No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA4380.18725070646527953No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT4120.17613536772533142No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT3690.1577523075015711No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC3400.14535442967624437No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC3320.14193432544856804No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC3150.13466660396475583No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA3080.13167401276553903No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA2950.126116343395565No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA2930.1252613173386459No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA2920.12483380431018636No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT2890.12355126522480772No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC2640.11286343951331917No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA2620.11200841345640009No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT2580.11029836134256192No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC2560.10944333528564282No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA2530.10816079620026421No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT2420.10345815288720923No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG2360.10089307471645198No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTT303.5907954E-430.8333347
CCGTTTA303.5907954E-430.83333427
GCCCATA250.00548750829.59999826
ATAGGAG250.00548750829.5999983
TAAGTCT250.00548750829.59999835
CCCATAG250.00548750829.59999827
TCAGTAC250.00548750829.5999983
TTAGGAC405.9202284E-527.7500023
GGTATCA3250.027.3230761
GTTCTGA358.848201E-426.4285721
ACGGGTC358.848201E-426.42857217
GTTACGG358.848201E-426.42857217
CCATAGA358.848201E-426.42857228
AATTCAT358.848201E-426.4285725
CTTAGGA358.848201E-426.4285722
TAGGACC451.3188153E-424.6666664
AATGATG400.001926616223.1250025
CTTCTAA400.001926616223.1250021
ATGCCGT502.6931963E-422.19999934
CACGAGA603.7099773E-521.58333411
Produced by FastQC (version 0.11.5)