##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632734.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	233911
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.94779210896452	34.0	33.0	34.0	31.0	34.0
2	33.08841396941572	34.0	33.0	34.0	31.0	34.0
3	33.168132323832566	34.0	33.0	34.0	31.0	34.0
4	36.48030661234401	37.0	37.0	37.0	35.0	37.0
5	36.44304457678348	37.0	37.0	37.0	35.0	37.0
6	36.4889124496069	37.0	37.0	37.0	35.0	37.0
7	36.48877137030751	37.0	37.0	37.0	35.0	37.0
8	36.47141861648234	37.0	37.0	37.0	35.0	37.0
9	38.25342972327082	39.0	39.0	39.0	37.0	39.0
10	38.279478092094855	39.0	39.0	39.0	37.0	39.0
11	38.351501211999434	39.0	39.0	39.0	37.0	39.0
12	38.29996451641864	39.0	39.0	39.0	37.0	39.0
13	38.34664038886585	39.0	39.0	39.0	37.0	39.0
14	39.8503319638666	41.0	40.0	41.0	38.0	41.0
15	39.847873763952954	41.0	40.0	41.0	38.0	41.0
16	39.806067264899895	41.0	40.0	41.0	38.0	41.0
17	39.80613994211473	41.0	40.0	41.0	38.0	41.0
18	39.81325803403859	41.0	40.0	41.0	38.0	41.0
19	39.813296510211146	41.0	40.0	41.0	38.0	41.0
20	39.799150104099425	41.0	40.0	41.0	38.0	41.0
21	39.75949399557952	41.0	40.0	41.0	38.0	41.0
22	39.718799030400454	41.0	40.0	41.0	38.0	41.0
23	39.68978799628918	41.0	40.0	41.0	38.0	41.0
24	39.674649759951436	41.0	40.0	41.0	37.0	41.0
25	39.624515307103984	41.0	40.0	41.0	37.0	41.0
26	39.53480597321203	41.0	40.0	41.0	37.0	41.0
27	39.43394709953786	41.0	39.0	41.0	37.0	41.0
28	39.39583431305069	41.0	39.0	41.0	37.0	41.0
29	39.35458358093463	41.0	39.0	41.0	36.0	41.0
30	39.31489754650273	41.0	39.0	41.0	36.0	41.0
31	39.25018917451509	41.0	39.0	41.0	36.0	41.0
32	39.198460098071486	41.0	39.0	41.0	36.0	41.0
33	39.12406855598925	41.0	39.0	41.0	35.0	41.0
34	39.09107310045274	40.0	39.0	41.0	35.0	41.0
35	39.00006840208455	40.0	39.0	41.0	35.0	41.0
36	38.954076550482874	40.0	39.0	41.0	35.0	41.0
37	38.8898512682174	40.0	39.0	41.0	35.0	41.0
38	38.8200255652791	40.0	38.0	41.0	35.0	41.0
39	38.72468588480234	40.0	38.0	41.0	35.0	41.0
40	38.643975700159466	40.0	38.0	41.0	35.0	41.0
41	38.59412340591079	40.0	38.0	41.0	35.0	41.0
42	38.51471286087443	40.0	38.0	41.0	35.0	41.0
43	37.77184057184143	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	4.0
19	3.0
20	17.0
21	30.0
22	46.0
23	75.0
24	133.0
25	221.0
26	300.0
27	414.0
28	641.0
29	854.0
30	1095.0
31	1467.0
32	2018.0
33	2661.0
34	3923.0
35	6346.0
36	10077.0
37	19627.0
38	50532.0
39	133425.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	44.005198558426066	15.518295420052928	12.148210216706353	28.32829580481465
2	22.065229937882357	18.749438889150145	30.48167892916537	28.70365224380213
3	22.03786910406095	18.82168859095981	28.102996438816476	31.03744586616277
4	16.26174057654407	14.932175058034893	33.07454544677249	35.731538918648546
5	17.698611865196593	32.54485680451112	32.49612031926673	17.26041101102556
6	35.82559178490964	32.817610116668305	14.427282171424174	16.929515926997876
7	31.05454638730115	26.69690608821304	18.88581554522874	23.362731979257067
8	28.698522087460614	29.190589583217548	19.57582157316244	22.535066756159395
9	28.185933966337622	12.302542419980249	16.610591207767055	42.90093240591507
10	20.36201803249954	22.18365104676565	28.323165648473136	29.131165272261672
11	40.872810598903	18.675479135226645	17.702886995481187	22.748823270389167
12	23.961677732128887	22.88135230921162	25.50799235606705	27.64897760259244
13	36.736622048556924	16.74525781173181	19.465523211819878	27.052596927891376
14	24.90947411622369	18.984998567831354	21.667215308386524	34.43831200755843
15	30.406436636156485	23.171633655535654	18.750293915207067	27.671635793100798
16	26.826442535836282	22.918118429659142	21.440633403302968	28.81480563120161
17	28.270154032944156	22.59449106711527	20.49283701920816	28.642517880732417
18	27.468567104582515	20.35261274587343	22.429043525101427	29.74977662444263
19	29.69377241771443	21.413700082510015	21.86515384056329	27.027373659212266
20	31.586372594704827	19.64208609257367	20.872896101508694	27.898645211212813
21	28.872519890043648	20.783973391589107	20.860070710654906	29.48343600771233
22	29.527469849643666	21.818982433489662	20.039673209041045	28.613874507825628
23	29.657006297266907	20.937878081834544	20.49326453223662	28.911851088661926
24	28.884490254840518	20.363728084613378	21.477827036778947	29.273954623767157
25	29.475313260171603	21.075964790026976	21.189255742568754	28.259466207232663
26	29.370145055170553	21.485949784319676	21.10802826716144	28.035876893348327
27	27.923013453835004	20.647169222482056	22.971130045188126	28.45868727849481
28	27.531839032794526	22.00623313995494	20.739939549657777	29.721988277592757
29	28.508278789796123	22.806110016202744	20.983621975879714	27.70198921812142
30	28.224410138898982	20.99003467130661	22.265306035201423	28.520249154592985
31	28.465954999978628	21.676193081984174	21.80401947749358	28.053832440543623
32	26.386531629551413	20.878881283907127	22.215287010871656	30.519300075669808
33	26.925198045410433	21.131968996755177	23.34349389297639	28.599339064858004
34	27.510035868343085	20.384248709979435	23.098528927669072	29.0071864940084
35	26.579767518415125	21.691583551008716	23.648738195296502	28.079910735279658
36	26.517778129288487	20.80876914723976	24.82653658870254	27.846916134769206
37	26.879454151365263	19.85926271103112	24.607222405102796	28.654060732500824
38	25.877791125684556	19.456545438222232	25.84359008340779	28.822073352685422
39	25.053973519843016	18.43307924809009	27.194531253339942	29.318415978726954
40	24.711108071018465	19.051263087242585	28.624562333537114	27.61306650820184
41	22.895032726122327	18.557058026343356	29.551838092265864	28.996071155268453
42	21.339740328586515	19.41165657023398	30.9579284428693	28.29067465831021
43	20.781835826446812	19.713480768326413	30.230728781459614	29.273954623767157
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3.0
1	1.5
2	0.0
3	0.0
4	0.0
5	0.0
6	5.0
7	10.0
8	8.0
9	6.0
10	13.5
11	21.0
12	21.0
13	34.0
14	47.0
15	86.0
16	125.0
17	129.0
18	133.0
19	133.0
20	154.5
21	176.0
22	160.5
23	145.0
24	192.5
25	240.0
26	240.0
27	301.5
28	363.0
29	460.5
30	558.0
31	728.0
32	898.0
33	898.0
34	1266.0
35	1634.0
36	1942.5
37	2251.0
38	3025.0
39	3799.0
40	3799.0
41	4661.0
42	5523.0
43	6795.0
44	8067.0
45	11051.5
46	14036.0
47	14036.0
48	16595.0
49	19154.0
50	21285.5
51	23417.0
52	24563.5
53	25710.0
54	25710.0
55	24636.5
56	23563.0
57	22979.5
58	22396.0
59	22090.0
60	21784.0
61	21784.0
62	20044.5
63	18305.0
64	15848.5
65	13392.0
66	11641.0
67	9890.0
68	9890.0
69	8524.0
70	7158.0
71	6062.0
72	4966.0
73	3894.0
74	2822.0
75	2822.0
76	2154.0
77	1486.0
78	1149.0
79	812.0
80	647.5
81	483.0
82	483.0
83	377.5
84	272.0
85	200.5
86	129.0
87	106.0
88	83.0
89	83.0
90	59.0
91	35.0
92	23.5
93	12.0
94	8.0
95	4.0
96	4.0
97	2.5
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	233911.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.728371397055646
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.72261706989677	41.63389395985297
2	7.429444864776275	7.389083870190849
3	2.522114759193565	3.7626197835351953
4	1.4160598358052017	2.816733977414988
5	0.9088051859177338	2.2596700906443634
6	0.5985961675480539	1.7860327522010264
7	0.46820183768382373	1.6298040411187604
8	0.3210560130576794	1.2772474125272182
9	0.27830892145229785	1.245586446818447
>10	2.0177244589565526	19.657377704227848
>50	0.21469376587398736	7.313314358319695
>100	0.10038921459848482	8.662689067579816
>500	0.001987905239573957	0.5659465355688058
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	633	0.2706157470148903	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	512	0.21888667057128564	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	438	0.18725070646527953	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	412	0.17613536772533142	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	369	0.1577523075015711	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	340	0.14535442967624437	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	332	0.14193432544856804	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	315	0.13466660396475583	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	308	0.13167401276553903	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	295	0.126116343395565	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	293	0.1252613173386459	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	292	0.12483380431018636	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	289	0.12355126522480772	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	264	0.11286343951331917	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	262	0.11200841345640009	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	258	0.11029836134256192	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	256	0.10944333528564282	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	253	0.10816079620026421	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	242	0.10345815288720923	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	236	0.10089307471645198	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	4.275130284595423E-4	0.0	0.0	0.0	0.0
7	4.275130284595423E-4	0.0	0.0	0.0	0.0
8	8.550260569190846E-4	0.0	0.0	0.0	0.0
9	8.550260569190846E-4	0.0	0.0	0.0	0.0
10	8.550260569190846E-4	0.0	0.0	0.0	0.0
11	8.550260569190846E-4	0.0	0.0	0.0	0.0
12	8.550260569190846E-4	0.0	0.0	0.0	0.0
13	8.550260569190846E-4	0.0	0.0	0.0	0.0
14	8.550260569190846E-4	0.0	0.0	0.0	0.0
15	8.550260569190846E-4	0.0	0.0	0.0	0.0
16	8.550260569190846E-4	0.0	0.0	0.0	0.0
17	8.550260569190846E-4	0.0	0.0	4.275130284595423E-4	0.0
18	8.550260569190846E-4	0.0	0.0	8.550260569190846E-4	0.0
19	8.550260569190846E-4	0.0	0.0	8.550260569190846E-4	0.0
20	8.550260569190846E-4	0.0	0.0	0.001282539085378627	0.0
21	8.550260569190846E-4	0.0	0.0	0.0021375651422977115	0.0
22	8.550260569190846E-4	0.0	0.0	0.004702643313054965	0.0
23	8.550260569190846E-4	0.0	0.0	0.005985182398433592	0.0
24	8.550260569190846E-4	0.0	0.0	0.006840208455352677	0.0
25	8.550260569190846E-4	0.0	0.0	0.010260312683029016	0.0
26	8.550260569190846E-4	0.0	0.0	0.015817982053003065	0.0
27	8.550260569190846E-4	0.0	0.0	0.052584102500523706	0.0
28	8.550260569190846E-4	0.0	0.0	0.23384962656736963	0.0
29	8.550260569190846E-4	0.0	0.0	0.4980526781553668	0.0
30	8.550260569190846E-4	0.0	0.0	0.8058620586462373	0.0
31	8.550260569190846E-4	0.0	0.0	1.5749579968449539	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACCGTT	30	3.5907954E-4	30.833334	7
CCGTTTA	30	3.5907954E-4	30.833334	27
GCCCATA	25	0.005487508	29.599998	26
ATAGGAG	25	0.005487508	29.599998	3
TAAGTCT	25	0.005487508	29.599998	35
CCCATAG	25	0.005487508	29.599998	27
TCAGTAC	25	0.005487508	29.599998	3
TTAGGAC	40	5.9202284E-5	27.750002	3
GGTATCA	325	0.0	27.323076	1
GTTCTGA	35	8.848201E-4	26.428572	1
ACGGGTC	35	8.848201E-4	26.428572	17
GTTACGG	35	8.848201E-4	26.428572	17
CCATAGA	35	8.848201E-4	26.428572	28
AATTCAT	35	8.848201E-4	26.428572	5
CTTAGGA	35	8.848201E-4	26.428572	2
TAGGACC	45	1.3188153E-4	24.666666	4
AATGATG	40	0.0019266162	23.125002	5
CTTCTAA	40	0.0019266162	23.125002	1
ATGCCGT	50	2.6931963E-4	22.199999	34
CACGAGA	60	3.7099773E-5	21.583334	11
>>END_MODULE