FastQCFastQC Report
Fri 10 Feb 2017
ERR1632733.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632733.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65960
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA1380.20921770770163736No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA1220.18496058217101272No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT1010.15312310491206793No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1000.1516070345664039No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA920.13947847180109157No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT840.12734990903577925No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC790.11976955730745907No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA680.10309278350515465No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA658.367351E-1131.3076921
CAGCAGT303.563136E-430.83333221
CTGTCCG250.005459320329.611
TCCGATT358.780686E-426.4285717
TTCCGAT358.780686E-426.4285716
GTAATTC358.780686E-426.4285712
ATTCCGA358.780686E-426.4285715
TACGCCC358.780686E-426.428574
TAATTCC358.780686E-426.4285713
AATTCCG358.780686E-426.4285714
AGTAATT358.780686E-426.4285711
TTATACA451.3048308E-424.66666637
CCGATTA400.001912054623.12500218
GTCCAGA400.001912054623.1250021
CAGTAAT400.001912054623.12500210
ACGCCCA450.003788266820.5555555
ACGGGAG450.003788266820.55555513
CTTATAC909.14315E-820.55555537
TTTACGC555.0727994E-420.1818182
GTATCAA1355.2750693E-1119.1851861
Produced by FastQC (version 0.11.5)