##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632733.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	65960
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.016585809581564	34.0	33.0	34.0	31.0	34.0
2	33.15708004851425	34.0	33.0	34.0	31.0	34.0
3	33.233535476046086	34.0	34.0	34.0	31.0	34.0
4	36.53446027895694	37.0	37.0	37.0	35.0	37.0
5	36.50180412371134	37.0	37.0	37.0	35.0	37.0
6	36.540327471194665	37.0	37.0	37.0	35.0	37.0
7	36.53956943602183	37.0	37.0	37.0	35.0	37.0
8	36.521012734990904	37.0	37.0	37.0	35.0	37.0
9	38.31502425712553	39.0	39.0	39.0	37.0	39.0
10	38.35371437234688	39.0	39.0	39.0	37.0	39.0
11	38.405397210430564	39.0	39.0	39.0	37.0	39.0
12	38.36315949060037	39.0	39.0	39.0	37.0	39.0
13	38.397377198302	39.0	39.0	39.0	37.0	39.0
14	39.93370224378411	41.0	40.0	41.0	38.0	41.0
15	39.937431776834444	41.0	40.0	41.0	38.0	41.0
16	39.89382959369315	41.0	40.0	41.0	38.0	41.0
17	39.90410855063675	41.0	40.0	41.0	38.0	41.0
18	39.89128259551243	41.0	40.0	41.0	38.0	41.0
19	39.900758035172835	41.0	40.0	41.0	38.0	41.0
20	39.89191934505761	41.0	40.0	41.0	38.0	41.0
21	39.83612795633717	41.0	40.0	41.0	38.0	41.0
22	39.81099151000606	41.0	40.0	41.0	38.0	41.0
23	39.7740903577926	41.0	40.0	41.0	38.0	41.0
24	39.759081261370525	41.0	40.0	41.0	38.0	41.0
25	39.700485142510615	41.0	40.0	41.0	38.0	41.0
26	39.62410551849606	41.0	40.0	41.0	37.0	41.0
27	39.55882352941177	41.0	40.0	41.0	37.0	41.0
28	39.514144936325046	41.0	40.0	41.0	37.0	41.0
29	39.46729836264403	41.0	39.0	41.0	37.0	41.0
30	39.40573074590661	41.0	39.0	41.0	36.0	41.0
31	39.366040024257124	41.0	39.0	41.0	36.0	41.0
32	39.3	41.0	39.0	41.0	36.0	41.0
33	39.24599757428744	41.0	39.0	41.0	36.0	41.0
34	39.20979381443299	41.0	39.0	41.0	35.0	41.0
35	39.12230139478472	41.0	39.0	41.0	35.0	41.0
36	39.07075500303214	41.0	39.0	41.0	35.0	41.0
37	39.04219223771983	41.0	39.0	41.0	35.0	41.0
38	38.94842328684051	40.0	39.0	41.0	35.0	41.0
39	38.88365676167374	40.0	39.0	41.0	35.0	41.0
40	38.80888417222559	40.0	38.0	41.0	35.0	41.0
41	38.76726804123712	40.0	38.0	41.0	35.0	41.0
42	38.70093996361431	40.0	38.0	41.0	35.0	41.0
43	37.97662219526986	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	4.0
20	4.0
21	10.0
22	13.0
23	19.0
24	41.0
25	60.0
26	70.0
27	115.0
28	151.0
29	222.0
30	262.0
31	351.0
32	482.0
33	714.0
34	1048.0
35	1617.0
36	2607.0
37	5101.0
38	13161.0
39	39907.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.41267434808975	15.900545785324438	12.472710733778047	28.21406913280776
2	21.029411764705884	19.41024863553669	32.090661006670715	27.469678593086723
3	20.509399636143115	19.60582171012735	29.254093389933296	30.63068526379624
4	15.453305033353548	15.486658580958157	33.92813826561552	35.13189812007277
5	16.453911461491813	33.433899332929045	33.724984839296546	16.387204366282596
6	35.421467556094605	33.95997574287447	14.442086112795632	16.176470588235293
7	30.63068526379624	27.689508793208006	19.536082474226806	22.14372346876895
8	27.88204972710734	30.983929654335963	19.78623408126137	21.34778653729533
9	28.19436021831413	12.772892662219526	16.79805942995755	42.234687689508796
10	19.705882352941178	23.038204972710734	29.5194057004245	27.736506973923593
11	40.05154639175258	19.029714978775015	18.50970285021225	22.409035779260158
12	23.406610066707096	23.8068526379624	25.999090357792603	26.7874469375379
13	35.18647665251668	17.201334141904184	20.76106731352335	26.85112189205579
14	24.44511825348696	18.828077622801697	22.828987265009097	33.89781685870224
15	29.261673741661614	23.785627653123104	19.93480897513645	27.017889630078834
16	26.522134627046697	23.711340206185564	21.760157671315948	28.00636749545179
17	27.580351728320196	23.302001212856275	21.31746513038205	27.800181928441482
18	26.819284414796847	21.4038811400849	23.414190418435414	28.362644026682837
19	29.197998787143725	21.575197089144936	22.645542753183747	26.581261370527592
20	30.183444511825346	20.46543359611886	22.283201940570045	27.06791995148575
21	27.951788963007886	21.823832625833838	21.376591873862946	28.847786537295327
22	28.673438447543965	22.5591267434809	20.641297756215888	28.126137052759248
23	28.820497271073375	22.268041237113405	21.34778653729533	27.563674954517893
24	28.55518496058217	21.57974530018193	21.83141297756216	28.033656761673743
25	29.013038204972712	21.72377198302001	22.433292904790783	26.829896907216494
26	28.57337780473014	22.2892662219527	21.902668283808367	27.234687689508792
27	27.819890842935113	21.593389933292904	22.98817465130382	27.598544572468164
28	26.58429351121892	22.5	22.0573074590661	28.858399029714978
29	27.43177683444512	23.259551243177683	21.94511825348696	27.36355366889024
30	27.280169799878713	22.18162522741055	23.256519102486354	27.281685870224376
31	27.891146149181324	22.026986052152818	22.34536082474227	27.736506973923593
32	24.986355366889022	21.926925409338992	23.174651303820497	29.912067919951486
33	26.24924196482717	21.64038811400849	23.875075803517284	28.235294117647058
34	26.744996967859308	21.428138265615523	23.768950879320798	28.057913887204368
35	26.17495451788963	22.6728320194057	24.454214675560944	26.697998787143725
36	25.63523347483323	21.634323832625835	25.45027289266222	27.280169799878713
37	26.7495451788963	20.970285021224985	24.484536082474225	27.79563371740449
38	25.175864160097028	20.677683444511825	26.13250454821104	28.01394784718011
39	24.49060036385688	19.705882352941178	27.201334141904184	28.602183141297754
40	24.093389933292904	20.274408732565192	28.923590054578536	26.70861127956337
41	22.9214675560946	19.54972710733778	29.46634323832626	28.062462098241358
42	21.346270466949665	20.374469375379018	30.571558520315346	27.707701637355974
43	20.489690721649485	20.291085506367494	30.49120679199515	28.72801697998787
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	1.0
6	2.0
7	3.0
8	4.5
9	6.0
10	6.5
11	7.0
12	7.0
13	11.5
14	16.0
15	31.0
16	46.0
17	47.0
18	48.0
19	48.0
20	57.5
21	67.0
22	59.5
23	52.0
24	74.5
25	97.0
26	97.0
27	131.5
28	166.0
29	244.0
30	322.0
31	379.5
32	437.0
33	437.0
34	601.0
35	765.0
36	877.0
37	989.0
38	1275.0
39	1561.0
40	1561.0
41	1975.0
42	2389.0
43	2571.5
44	2754.0
45	3517.0
46	4280.0
47	4280.0
48	4899.0
49	5518.0
50	6197.5
51	6877.0
52	7412.0
53	7947.0
54	7947.0
55	7325.0
56	6703.0
57	6395.0
58	6087.0
59	5622.0
60	5157.0
61	5157.0
62	4742.5
63	4328.0
64	3695.0
65	3062.0
66	2562.5
67	2063.0
68	2063.0
69	1773.5
70	1484.0
71	1304.5
72	1125.0
73	871.0
74	617.0
75	617.0
76	520.5
77	424.0
78	338.5
79	253.0
80	204.5
81	156.0
82	156.0
83	111.5
84	67.0
85	53.0
86	39.0
87	31.0
88	23.0
89	23.0
90	17.5
91	12.0
92	11.0
93	10.0
94	6.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	65960.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.58580958156458
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.12431693989072	58.01243177683445
2	5.972222222222222	7.953305033353547
3	2.2222222222222223	4.439053972104306
4	1.2568306010928962	3.347483323226198
5	0.744535519125683	2.4787750151607035
6	0.4508196721311476	1.801091570648878
7	0.377959927140255	1.761673741661613
8	0.30965391621129323	1.6494845360824744
9	0.20719489981785066	1.2416616130988478
>10	1.2795992714025501	14.75591267434809
>50	0.04781420765027322	2.0118253486961795
>100	0.006830601092896175	0.547301394784718
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	138	0.20921770770163736	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	122	0.18496058217101272	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	101	0.15312310491206793	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	100	0.1516070345664039	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	92	0.13947847180109157	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	84	0.12734990903577925	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	79	0.11976955730745907	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	68	0.10309278350515465	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0015160703456640388	0.0
11	0.0	0.0	0.0	0.0015160703456640388	0.0
12	0.0	0.0	0.0	0.0015160703456640388	0.0
13	0.0	0.0	0.0	0.0015160703456640388	0.0
14	0.0	0.0	0.0	0.0015160703456640388	0.0
15	0.0	0.0	0.0	0.0015160703456640388	0.0
16	0.0	0.0	0.0	0.0030321406913280777	0.0
17	0.0	0.0	0.0	0.0030321406913280777	0.0
18	0.0	0.0	0.0	0.0030321406913280777	0.0
19	0.0	0.0	0.0	0.0030321406913280777	0.0
20	0.0	0.0	0.0	0.004548211036992117	0.0
21	0.0	0.0	0.0	0.01212856276531231	0.0
22	0.0	0.0	0.0	0.01364463311097635	0.0
23	0.0	0.0	0.0	0.015160703456640388	0.0
24	0.0	0.0	0.0	0.019708914493632504	0.0
25	0.0	0.0	0.0	0.021224984839296544	0.0
26	0.0	0.0	0.0	0.02577319587628866	0.0
27	0.0	0.0	0.0	0.03941782898726501	0.0
28	0.0	0.0	0.0	0.17889630078835658	0.0
29	0.0	0.0	0.0	0.3759854457246816	0.0
30	0.0	0.0	0.0	0.6064281382656155	0.0
31	0.0	0.0	0.0	1.3038204972710734	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	65	8.367351E-11	31.307692	1
CAGCAGT	30	3.563136E-4	30.833332	21
CTGTCCG	25	0.0054593203	29.6	11
TCCGATT	35	8.780686E-4	26.42857	17
TTCCGAT	35	8.780686E-4	26.42857	16
GTAATTC	35	8.780686E-4	26.42857	12
ATTCCGA	35	8.780686E-4	26.42857	15
TACGCCC	35	8.780686E-4	26.42857	4
TAATTCC	35	8.780686E-4	26.42857	13
AATTCCG	35	8.780686E-4	26.42857	14
AGTAATT	35	8.780686E-4	26.42857	11
TTATACA	45	1.3048308E-4	24.666666	37
CCGATTA	40	0.0019120546	23.125002	18
GTCCAGA	40	0.0019120546	23.125002	1
CAGTAAT	40	0.0019120546	23.125002	10
ACGCCCA	45	0.0037882668	20.555555	5
ACGGGAG	45	0.0037882668	20.555555	13
CTTATAC	90	9.14315E-8	20.555555	37
TTTACGC	55	5.0727994E-4	20.181818	2
GTATCAA	135	5.2750693E-11	19.185186	1
>>END_MODULE