FastQCFastQC Report
Fri 10 Feb 2017
ERR1632732.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632732.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1505389
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA39930.26524705574439567No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA36830.24465437172717483No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA27240.18094990729970792No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA26380.17523709818525313No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA23100.15344870993477433No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA21650.1438166480557517No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT21480.1426873718354525No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA19970.1326567418786772No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCGGGCTGCGTCT19530.12973390930849102No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC19420.1290032011659445No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC19410.1289367731529857No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG19070.1266782207123873No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT19050.12654536468646974No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA19010.12627965263463464No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA18580.12342324807740723No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA17740.11784329498886999No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC17310.11498689043164259No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC17250.11458832235388992No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT17210.11432261030205483No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGGTCTT17110.11365833017246704No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT17100.11359190215950828No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC16790.1115326337577862No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT16720.11106763766707477No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA16610.11033692952452821No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA16550.10993836144677556No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT16510.10967264939494045No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA16260.10801194907097103No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA15920.10575339663037261No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG15740.10455769239711463No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC15650.10395984028048563No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT15170.10077129565846435No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19500.030.4538461
ATACGGC3300.025.22727229
CTAAGTC1850.023.034
TACGGCT3800.022.39473730
TAGGTTG502.702641E-422.25
CAGGACA7650.022.0065364
GCGGTAA17950.021.3342623
CCAGGAC7400.020.753
CTCGTAT1251.8189894E-1120.7229
CGCGGTA18550.020.64420522
TATTAGA909.4831194E-820.5555572
GACAGGC7150.020.440567
GGTAATA19100.020.34031325
CGTGCCA19350.020.17312610
AGCCGCG19550.019.87212219
CCGCTTA851.2458331E-619.58823625
CGGTAAT19850.019.4785924
GTATCAA30500.019.2885252
TCGTATG1355.638867E-1119.18518630
AACTCCG20100.019.1442785
Produced by FastQC (version 0.11.5)