FastQCFastQC Report
Fri 10 Feb 2017
ERR1632731.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632731.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3400
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA140.411764705882353No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT130.3823529411764706RNA PCR Primer, Index 17 (95% over 21bp)
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT110.3235294117647059No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA100.29411764705882354No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT90.2647058823529412No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC90.2647058823529412No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA80.2352941176470588No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG80.2352941176470588No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT80.2352941176470588No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA80.2352941176470588No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT70.2058823529411765No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA70.2058823529411765No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT60.17647058823529413No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC60.17647058823529413No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT60.17647058823529413No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG60.17647058823529413No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC60.17647058823529413No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT60.17647058823529413No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA60.17647058823529413No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT60.17647058823529413No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA50.14705882352941177No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC50.14705882352941177No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG50.14705882352941177No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA50.14705882352941177No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTC50.14705882352941177No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA50.14705882352941177No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA50.14705882352941177No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGAC50.14705882352941177No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC50.14705882352941177No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA40.1176470588235294No Hit
GTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTTAT40.1176470588235294No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA40.1176470588235294No Hit
TTATAACGGTTCATATCACCTTACCGACGCTTATCGCAGATTA40.1176470588235294No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC40.1176470588235294No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGACTG40.1176470588235294No Hit
AACCGCCTGCGTGCGCTTTACGCCCAGTAATTCCGATTAACGC40.1176470588235294No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC40.1176470588235294No Hit
GTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACA40.1176470588235294No Hit
CCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTC40.1176470588235294No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA40.1176470588235294No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCTGT40.1176470588235294No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC40.1176470588235294No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA40.1176470588235294No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC40.1176470588235294No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC40.1176470588235294No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATG40.1176470588235294No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA40.1176470588235294No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC40.1176470588235294No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGCT40.1176470588235294No Hit
GCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGAC40.1176470588235294No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG40.1176470588235294No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC40.1176470588235294No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG40.1176470588235294No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT40.1176470588235294No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA40.1176470588235294No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA40.1176470588235294No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA302.9058263E-430.8333341
TACGGGA250.004769526429.59999816
CAGAGTA357.174362E-426.4285711
AGAGTAC357.174362E-426.4285712
GCAGAGT357.174362E-426.4285710
AACGCAG400.001565196223.1257
ACGCAGA400.001565196223.1258
GTATCAA400.001565196223.1252
TCAACGC400.001565196223.1255
CGCAGAG400.001565196223.1259
TATCAAC400.001565196223.1253
ATCAACG400.001565196223.1254
CAACGCA400.001565196223.1256
Produced by FastQC (version 0.11.5)