##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632731.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3400
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.965	34.0	33.0	34.0	31.0	34.0
2	33.07147058823529	34.0	33.0	34.0	31.0	34.0
3	33.11558823529412	34.0	33.0	34.0	31.0	34.0
4	36.45764705882353	37.0	37.0	37.0	35.0	37.0
5	36.44705882352941	37.0	37.0	37.0	35.0	37.0
6	36.489117647058826	37.0	37.0	37.0	35.0	37.0
7	36.48088235294118	37.0	37.0	37.0	35.0	37.0
8	36.45970588235294	37.0	37.0	37.0	35.0	37.0
9	38.22470588235294	39.0	39.0	39.0	37.0	39.0
10	38.21941176470588	39.0	39.0	39.0	37.0	39.0
11	38.34235294117647	39.0	39.0	39.0	37.0	39.0
12	38.22941176470588	39.0	39.0	39.0	37.0	39.0
13	38.3014705882353	39.0	39.0	39.0	37.0	39.0
14	39.80117647058824	41.0	40.0	41.0	38.0	41.0
15	39.74411764705882	41.0	40.0	41.0	38.0	41.0
16	39.75588235294118	41.0	40.0	41.0	38.0	41.0
17	39.686470588235295	41.0	40.0	41.0	38.0	41.0
18	39.71382352941176	41.0	40.0	41.0	38.0	41.0
19	39.69294117647059	41.0	40.0	41.0	38.0	41.0
20	39.70294117647059	41.0	40.0	41.0	37.0	41.0
21	39.63676470588236	41.0	40.0	41.0	37.0	41.0
22	39.64147058823529	41.0	40.0	41.0	37.0	41.0
23	39.57205882352941	41.0	40.0	41.0	37.0	41.0
24	39.57029411764706	41.0	40.0	41.0	37.0	41.0
25	39.501176470588234	41.0	40.0	41.0	37.0	41.0
26	39.39147058823529	41.0	39.0	41.0	36.0	41.0
27	39.375882352941176	41.0	39.0	41.0	36.0	41.0
28	39.275882352941174	41.0	39.0	41.0	36.0	41.0
29	39.225	41.0	39.0	41.0	35.0	41.0
30	39.167941176470585	41.0	39.0	41.0	35.0	41.0
31	39.070882352941176	41.0	39.0	41.0	35.0	41.0
32	39.00558823529412	41.0	39.0	41.0	35.0	41.0
33	38.88117647058824	40.0	39.0	41.0	35.0	41.0
34	38.908235294117645	40.0	39.0	41.0	35.0	41.0
35	38.76764705882353	40.0	38.0	41.0	35.0	41.0
36	38.663529411764706	40.0	38.0	41.0	35.0	41.0
37	38.59411764705882	40.0	38.0	41.0	35.0	41.0
38	38.51529411764706	40.0	38.0	41.0	35.0	41.0
39	38.37735294117647	40.0	38.0	41.0	34.0	41.0
40	38.28823529411765	40.0	37.0	41.0	34.0	41.0
41	38.2464705882353	40.0	37.0	41.0	35.0	41.0
42	38.23352941176471	40.0	37.0	41.0	35.0	41.0
43	37.56117647058824	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	1.0
21	2.0
22	2.0
23	0.0
24	2.0
25	4.0
26	8.0
27	10.0
28	9.0
29	13.0
30	17.0
31	23.0
32	38.0
33	51.0
34	80.0
35	99.0
36	149.0
37	278.0
38	694.0
39	1918.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.1764705882353	16.205882352941174	13.323529411764707	27.294117647058826
2	23.411764705882355	19.38235294117647	30.323529411764703	26.882352941176467
3	22.35294117647059	19.558823529411764	29.764705882352942	28.32352941176471
4	16.823529411764707	16.147058823529413	34.411764705882355	32.61764705882353
5	17.264705882352942	33.73529411764706	31.61764705882353	17.38235294117647
6	32.70588235294118	33.73529411764706	15.411764705882353	18.14705882352941
7	28.705882352941174	28.794117647058826	19.235294117647058	23.26470588235294
8	28.852941176470587	30.58823529411765	19.264705882352942	21.294117647058826
9	29.323529411764703	12.088235294117647	17.0	41.588235294117645
10	20.941176470588236	21.705882352941178	27.88235294117647	29.470588235294116
11	38.91176470588235	18.764705882352942	18.941176470588236	23.38235294117647
12	22.970588235294116	23.5	25.941176470588236	27.588235294117645
13	37.70588235294118	17.088235294117645	18.941176470588236	26.26470588235294
14	25.441176470588232	20.97058823529412	20.941176470588236	32.64705882352941
15	30.558823529411764	22.941176470588236	18.88235294117647	27.61764705882353
16	25.705882352941178	23.647058823529413	22.11764705882353	28.52941176470588
17	28.058823529411764	23.705882352941178	20.852941176470587	27.382352941176467
18	28.000000000000004	18.91176470588235	22.558823529411764	30.529411764705884
19	29.52941176470588	20.88235294117647	24.029411764705884	25.558823529411768
20	33.1764705882353	18.970588235294116	20.88235294117647	26.97058823529412
21	30.205882352941178	20.764705882352942	20.764705882352942	28.264705882352942
22	29.058823529411764	21.5	19.61764705882353	29.823529411764703
23	29.02941176470588	21.676470588235293	20.411764705882355	28.88235294117647
24	28.47058823529412	20.0	21.352941176470587	30.176470588235293
25	29.794117647058822	20.97058823529412	22.08823529411765	27.147058823529413
26	30.411764705882355	21.941176470588236	21.352941176470587	26.294117647058822
27	26.0	23.588235294117645	23.88235294117647	26.529411764705884
28	25.647058823529413	22.558823529411764	20.794117647058822	31.0
29	27.764705882352942	23.235294117647058	21.11764705882353	27.88235294117647
30	27.88235294117647	20.97058823529412	23.26470588235294	27.88235294117647
31	28.38235294117647	23.11764705882353	22.411764705882355	26.088235294117645
32	26.705882352941174	20.38235294117647	22.38235294117647	30.529411764705884
33	26.676470588235297	22.970588235294116	23.47058823529412	26.882352941176467
34	27.294117647058826	21.14705882352941	22.558823529411764	28.999999999999996
35	25.794117647058822	24.147058823529413	23.764705882352942	26.294117647058822
36	26.5	22.705882352941174	22.852941176470587	27.941176470588236
37	27.41176470588235	21.41176470588235	24.205882352941178	26.97058823529412
38	26.147058823529413	20.11764705882353	25.38235294117647	28.352941176470587
39	25.61764705882353	20.02941176470588	26.647058823529413	27.705882352941174
40	24.088235294117645	21.235294117647058	27.97058823529412	26.705882352941174
41	23.52941176470588	20.647058823529413	28.000000000000004	27.823529411764707
42	20.294117647058822	21.558823529411764	29.08823529411765	29.058823529411764
43	19.735294117647058	21.294117647058826	29.411764705882355	29.558823529411764
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.5
11	1.0
12	1.0
13	1.0
14	1.0
15	2.0
16	3.0
17	3.5
18	4.0
19	4.0
20	8.0
21	12.0
22	10.0
23	8.0
24	10.5
25	13.0
26	13.0
27	8.5
28	4.0
29	8.5
30	13.0
31	12.5
32	12.0
33	12.0
34	19.5
35	27.0
36	27.5
37	28.0
38	41.0
39	54.0
40	54.0
41	68.0
42	82.0
43	101.5
44	121.0
45	177.0
46	233.0
47	233.0
48	263.0
49	293.0
50	339.0
51	385.0
52	378.5
53	372.0
54	372.0
55	367.0
56	362.0
57	352.5
58	343.0
59	324.5
60	306.0
61	306.0
62	274.5
63	243.0
64	201.5
65	160.0
66	128.5
67	97.0
68	97.0
69	84.0
70	71.0
71	58.0
72	45.0
73	37.0
74	29.0
75	29.0
76	21.5
77	14.0
78	16.5
79	19.0
80	18.5
81	18.0
82	18.0
83	15.5
84	13.0
85	8.0
86	3.0
87	5.5
88	8.0
89	8.0
90	5.0
91	2.0
92	1.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3400.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.1470588235294
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.17825934987766	75.88235294117646
2	5.8720727018525	9.88235294117647
3	1.9923103809856693	5.029411764705882
4	0.9437259699405802	3.176470588235294
5	0.3145753233135267	1.3235294117647058
6	0.27962250961202373	1.411764705882353
7	0.06990562740300593	0.411764705882353
8	0.13981125480601186	0.9411764705882352
9	0.06990562740300593	0.5294117647058824
>10	0.13981125480601186	1.411764705882353
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	14	0.411764705882353	No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT	13	0.3823529411764706	RNA PCR Primer, Index 17 (95% over 21bp)
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	11	0.3235294117647059	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	10	0.29411764705882354	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	9	0.2647058823529412	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	9	0.2647058823529412	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	8	0.2352941176470588	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	8	0.2352941176470588	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	8	0.2352941176470588	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	8	0.2352941176470588	No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT	7	0.2058823529411765	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	7	0.2058823529411765	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	6	0.17647058823529413	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	6	0.17647058823529413	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	6	0.17647058823529413	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	6	0.17647058823529413	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	6	0.17647058823529413	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	6	0.17647058823529413	No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA	6	0.17647058823529413	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	6	0.17647058823529413	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	5	0.14705882352941177	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	5	0.14705882352941177	No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG	5	0.14705882352941177	No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA	5	0.14705882352941177	No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTC	5	0.14705882352941177	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	5	0.14705882352941177	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	5	0.14705882352941177	No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGAC	5	0.14705882352941177	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	5	0.14705882352941177	No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA	4	0.1176470588235294	No Hit
GTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTTAT	4	0.1176470588235294	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	4	0.1176470588235294	No Hit
TTATAACGGTTCATATCACCTTACCGACGCTTATCGCAGATTA	4	0.1176470588235294	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	4	0.1176470588235294	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGACTG	4	0.1176470588235294	No Hit
AACCGCCTGCGTGCGCTTTACGCCCAGTAATTCCGATTAACGC	4	0.1176470588235294	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	4	0.1176470588235294	No Hit
GTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACA	4	0.1176470588235294	No Hit
CCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTC	4	0.1176470588235294	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	4	0.1176470588235294	No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCTGT	4	0.1176470588235294	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	4	0.1176470588235294	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	4	0.1176470588235294	No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC	4	0.1176470588235294	No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC	4	0.1176470588235294	No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATG	4	0.1176470588235294	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	4	0.1176470588235294	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	4	0.1176470588235294	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGCT	4	0.1176470588235294	No Hit
GCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGAC	4	0.1176470588235294	No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG	4	0.1176470588235294	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	4	0.1176470588235294	No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG	4	0.1176470588235294	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	4	0.1176470588235294	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	4	0.1176470588235294	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	4	0.1176470588235294	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.08823529411764706	0.0
29	0.0	0.0	0.0	0.35294117647058826	0.0
30	0.0	0.0	0.0	0.5882352941176471	0.0
31	0.0	0.0	0.0	1.1470588235294117	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	30	2.9058263E-4	30.833334	1
TACGGGA	25	0.0047695264	29.599998	16
CAGAGTA	35	7.174362E-4	26.42857	11
AGAGTAC	35	7.174362E-4	26.42857	12
GCAGAGT	35	7.174362E-4	26.42857	10
AACGCAG	40	0.0015651962	23.125	7
ACGCAGA	40	0.0015651962	23.125	8
GTATCAA	40	0.0015651962	23.125	2
TCAACGC	40	0.0015651962	23.125	5
CGCAGAG	40	0.0015651962	23.125	9
TATCAAC	40	0.0015651962	23.125	3
ATCAACG	40	0.0015651962	23.125	4
CAACGCA	40	0.0015651962	23.125	6
>>END_MODULE