FastQCFastQC Report
Fri 10 Feb 2017
ERR1632730.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632730.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences403737
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC8370.20731317664717377No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA8150.20186408478786935No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC8070.19988259683903134No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG7370.182544577286699No Hit
GATCAGCAGGCAGCAGCGATGGCTCCGAACCTCGCTCCTAAAG7270.18006771735065155No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG6920.17139870757448536No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA6600.16347275577913345No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT6340.15703291994541No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA5900.14613473622680112No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA5840.1446486202651726No Hit
CTGCTGATCCGCGCCTAGAGTTTGACCAGCCACTCTCCAGCTC5700.14118101635470617No Hit
GGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGG5470.13548423850179694No Hit
ACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGGCAGGACA5460.13523655250819222No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT5260.13028283263609725No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC5260.13028283263609725No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA5110.12656754273202606No Hit
GGTTCAGACAGCAGCTCTGCCAAGAAGTACTTGGCCAGTTCCT5070.12557679875760705No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG5040.12483374077679281No Hit
GTCCCTGGCTGCTGCCGCGGGGAAGCAGGAACTGGCCAAGTAC4820.11938464891748837No Hit
GCCAAGAAGTACTTGGCCAGTTCCTGCTTCCCCGCGGCAGCAG4730.11715547497504564No Hit
GTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCA4670.11566935901341714No Hit
ATGCTGTCCTGCCGCCTCCAGTGCGCGCTGGCTGCGCTGTCCA4540.11244944109655543No Hit
GTCCTGCCGCCTCCAGTGCGCGCTGGCTGCGCTGTCCATCGTC4490.11121101112853168No Hit
CGGCAGGACAGCATCTCGGCGCCGCGAAAGCCGAGCTGGAGAG4420.10947720917329846No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA4420.10947720917329846No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT4340.10749572122446048No Hit
GTTCCTGCTTCCCCGCGGCAGCAGCCAGGGACTTCTGCAGAAA4300.1065049772500415No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT4300.1065049772500415No Hit
GTTCGGAGCCATCGCTGCTGCCTGCTGATCCGCGCCTAGAGTT4250.10526654728201774No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC4240.105018861288413No Hit
AGTCTGGGGTCCGAGGGAGCGCCGGTGACACAGCCCAGGGCCA4130.10229431535876078No Hit
GAGCTGGAGAGTGGCTGGTCAAACTCTAGGCGCGGATCAGCAG4080.10105588539073704No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4150.029.867471
CTACCGT250.005492173629.5999988
GGACGTA453.999794E-628.77777727
CTGTCGC358.859389E-426.4285729
TTATGCG358.859389E-426.42857237
TACGGCT509.072028E-625.89999830
TATACAC1750.025.37142837
ACACGAA451.3211358E-424.66666617
CACGAAA451.3211358E-424.66666618
AAACACG451.3211358E-424.66666615
ATACGGC451.3211358E-424.66666629
TTAGCTA400.001929029223.1254
AGCGACG400.001929029223.12529
TAGCTAC400.001929029223.1255
GACGTAT502.6978969E-422.19999928
TAAACCA502.6978969E-422.1999995
ACGTATA502.6978969E-422.19999929
TATTAGC603.7185815E-521.5833342
TAATACT450.003821574420.5555554
GCGACGC450.003821574420.55555530
Produced by FastQC (version 0.11.5)