##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632730.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	403737
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.721524160530244	34.0	31.0	34.0	31.0	34.0
2	32.87928280043692	34.0	31.0	34.0	31.0	34.0
3	32.97067645521713	34.0	33.0	34.0	31.0	34.0
4	36.37611613500868	37.0	37.0	37.0	35.0	37.0
5	36.29609374419486	37.0	37.0	37.0	35.0	37.0
6	36.32022826741171	37.0	37.0	37.0	35.0	37.0
7	36.3101821235111	37.0	37.0	37.0	35.0	37.0
8	36.28213168473536	37.0	37.0	37.0	35.0	37.0
9	38.0548451095639	39.0	38.0	39.0	37.0	39.0
10	38.036969611405446	39.0	38.0	39.0	37.0	39.0
11	38.122086903107714	39.0	38.0	39.0	37.0	39.0
12	38.046599642836796	39.0	38.0	39.0	37.0	39.0
13	38.096713949922844	39.0	38.0	39.0	37.0	39.0
14	39.54453270322016	40.0	39.0	41.0	37.0	41.0
15	39.51787425972849	40.0	39.0	41.0	37.0	41.0
16	39.50074925013065	40.0	39.0	41.0	37.0	41.0
17	39.45797635589505	40.0	39.0	41.0	37.0	41.0
18	39.472431310481824	40.0	39.0	41.0	37.0	41.0
19	39.449032414665986	40.0	39.0	41.0	37.0	41.0
20	39.44261239371175	40.0	39.0	41.0	37.0	41.0
21	39.41101013778772	40.0	39.0	41.0	37.0	41.0
22	39.376076505249706	40.0	39.0	41.0	37.0	41.0
23	39.34560122059658	40.0	39.0	41.0	37.0	41.0
24	39.32019606823254	40.0	39.0	41.0	36.0	41.0
25	39.26898946591469	40.0	39.0	41.0	36.0	41.0
26	39.175842194299754	40.0	39.0	41.0	36.0	41.0
27	39.07636902240815	40.0	39.0	41.0	36.0	41.0
28	39.037445168513166	40.0	39.0	41.0	36.0	41.0
29	38.98740764408513	40.0	39.0	41.0	36.0	41.0
30	38.9383113264328	40.0	38.0	41.0	35.0	41.0
31	38.83527147623329	40.0	38.0	41.0	35.0	41.0
32	38.784547861602974	40.0	38.0	41.0	35.0	41.0
33	38.72207154657611	40.0	38.0	41.0	35.0	41.0
34	38.648756492469104	40.0	38.0	41.0	35.0	41.0
35	38.58032085243612	40.0	38.0	41.0	35.0	41.0
36	38.52119077518285	40.0	38.0	41.0	35.0	41.0
37	38.45847668160213	40.0	38.0	41.0	35.0	41.0
38	38.402375804050656	40.0	38.0	41.0	34.0	41.0
39	38.289849580296085	40.0	38.0	41.0	34.0	41.0
40	38.18366411797779	40.0	37.0	41.0	34.0	41.0
41	38.12279280818949	40.0	37.0	41.0	34.0	41.0
42	38.03929290602546	40.0	37.0	41.0	34.0	41.0
43	37.2274748165266	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	1.0
15	0.0
16	0.0
17	2.0
18	3.0
19	15.0
20	13.0
21	42.0
22	83.0
23	192.0
24	289.0
25	443.0
26	664.0
27	953.0
28	1305.0
29	1794.0
30	2554.0
31	3453.0
32	4637.0
33	6382.0
34	9001.0
35	13942.0
36	22802.0
37	45778.0
38	110106.0
39	179282.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.07389711619197	15.71270406229798	11.522352422492862	27.691046399017182
2	21.566514835152585	18.66091044417529	32.00226880370142	27.7703059169707
3	22.21867205631389	18.380529899414718	27.41388577217347	31.98691227209792
4	15.43777260939671	15.032558323859343	32.51720798440569	37.012461082338255
5	18.316131541077485	32.04784302652469	32.14815585393462	17.487869578463208
6	37.808028493796705	31.688202963810603	14.62585792236035	15.877910620032349
7	33.14360586223209	25.88417707567055	18.440717595860672	22.531499466236685
8	28.465312815025623	30.218434277760025	19.574128702595996	21.742124204618353
9	28.58246829000067	11.683100632342342	16.46393568090118	43.27049539675581
10	19.29944493568833	22.830951832504823	29.381750991363187	28.487852240443658
11	41.22188454365094	18.53037992554559	17.37690625332828	22.87082927747519
12	23.47196318395391	22.507969296844234	25.573083467702983	28.44698405149887
13	36.189152839596076	16.529324783212836	20.10591053086539	27.175611846325705
14	25.7306117596356	18.258420704567577	21.838226370136997	34.17274116565983
15	28.84873073312577	23.304527452277103	20.01327596925721	27.833465845339912
16	28.634977720644876	21.659397082754367	21.62348261368168	28.08214258291908
17	28.033100756185338	22.26474165112437	20.94482299120467	28.757334601485617
18	27.348496669861817	20.045475148425833	22.795532735419343	29.810495446293007
19	30.62092401736774	21.817420746674195	21.47214647158918	26.089508764368887
20	31.24756958118775	20.143806487886916	21.311150575746094	27.297473355179235
21	28.041274393974298	21.581623680762476	20.735776012602265	29.641325912660964
22	29.768636513373803	21.32873628129203	20.233468817571833	28.669158387762327
23	27.664048625714265	21.506079452713028	21.510042428610703	29.319829492962
24	28.602035483495442	21.07634425380879	21.162538979583243	29.15908128311252
25	29.009726628968856	21.247247589396068	21.985847222325425	27.757178559309647
26	29.255678820618375	21.719832465193925	20.27111708859976	28.753371625587942
27	29.001057619192693	21.09516838932275	21.574193100954332	28.32958089053022
28	27.600145639364236	22.65014105717336	20.862591241327895	28.88712206213451
29	28.865573380690897	22.788845213592015	21.536544829926413	26.809036575790675
30	28.886379004153696	20.658002610610374	21.70497130557764	28.750647079658293
31	27.219452267193745	20.9413553872942	21.889497370813178	29.949694974698875
32	26.072170744816553	21.667075348556114	22.330873811416836	29.9298800952105
33	27.351964273772282	20.606483923940587	23.184647431372404	28.856904370914727
34	28.00189232099114	20.662708644488863	23.62007940812955	27.71531962639045
35	27.648939780104374	21.000552339765736	22.788102155611202	28.562405724518687
36	26.29137284915675	20.166593599298555	23.622308582071994	29.9197249694727
37	25.85767467435484	19.90503719005194	25.501006843564006	28.736281292029215
38	25.573578839690192	19.07281225153999	25.240193492298204	30.11341541647161
39	24.688844470534036	19.04383299028823	28.15322846308364	28.114094076094094
40	25.900276665254857	18.78401038299685	27.795569888318383	27.52014306342991
41	22.925072510074628	19.05200662807719	29.15412756324043	28.86879329860776
42	21.017642673324467	20.297124117928256	30.36085372408276	28.32437948466452
43	21.169474187404177	19.486943232847125	29.377540329471906	29.966042250276786
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	1.5
2	1.0
3	7.0
4	13.0
5	13.0
6	13.0
7	13.0
8	16.5
9	20.0
10	25.0
11	30.0
12	30.0
13	66.5
14	103.0
15	168.0
16	233.0
17	243.0
18	253.0
19	253.0
20	303.0
21	353.0
22	334.0
23	315.0
24	369.5
25	424.0
26	424.0
27	476.0
28	528.0
29	752.0
30	976.0
31	1289.5
32	1603.0
33	1603.0
34	2882.0
35	4161.0
36	4637.0
37	5113.0
38	6393.0
39	7673.0
40	7673.0
41	8878.5
42	10084.0
43	12729.5
44	15375.0
45	19096.5
46	22818.0
47	22818.0
48	26194.5
49	29571.0
50	33110.0
51	36649.0
52	41028.0
53	45407.0
54	45407.0
55	43849.5
56	42292.0
57	38801.5
58	35311.0
59	36523.5
60	37736.0
61	37736.0
62	34971.5
63	32207.0
64	25695.0
65	19183.0
66	17891.5
67	16600.0
68	16600.0
69	14771.5
70	12943.0
71	13118.5
72	13294.0
73	10661.0
74	8028.0
75	8028.0
76	4988.5
77	1949.0
78	1526.5
79	1104.0
80	897.0
81	690.0
82	690.0
83	526.5
84	363.0
85	267.5
86	172.0
87	129.0
88	86.0
89	86.0
90	65.5
91	45.0
92	30.5
93	16.0
94	8.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	403737.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.22361524596595
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.4553640188435	37.79145139073297
2	6.270340891327243	5.545942860782064
3	2.2374297959733305	2.96841703310954
4	1.2436913231528979	2.2000210623944025
5	0.8372743171154406	1.8513648627721067
6	0.523796393932003	1.3898502115483988
7	0.42603656414207813	1.318861396533279
8	0.32632552342430354	1.154503551428388
9	0.2508115831992402	0.9982615459171298
>10	1.9607570656622024	17.14210563242793
>50	0.25490643392089535	7.9527335255548115
>100	0.20313735683320797	16.86951016012769
>500	0.01012873247367796	2.8169767666712704
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC	837	0.20731317664717377	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	815	0.20186408478786935	No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC	807	0.19988259683903134	No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG	737	0.182544577286699	No Hit
GATCAGCAGGCAGCAGCGATGGCTCCGAACCTCGCTCCTAAAG	727	0.18006771735065155	No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG	692	0.17139870757448536	No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA	660	0.16347275577913345	No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT	634	0.15703291994541	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	590	0.14613473622680112	No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA	584	0.1446486202651726	No Hit
CTGCTGATCCGCGCCTAGAGTTTGACCAGCCACTCTCCAGCTC	570	0.14118101635470617	No Hit
GGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGG	547	0.13548423850179694	No Hit
ACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGGCAGGACA	546	0.13523655250819222	No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT	526	0.13028283263609725	No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC	526	0.13028283263609725	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	511	0.12656754273202606	No Hit
GGTTCAGACAGCAGCTCTGCCAAGAAGTACTTGGCCAGTTCCT	507	0.12557679875760705	No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG	504	0.12483374077679281	No Hit
GTCCCTGGCTGCTGCCGCGGGGAAGCAGGAACTGGCCAAGTAC	482	0.11938464891748837	No Hit
GCCAAGAAGTACTTGGCCAGTTCCTGCTTCCCCGCGGCAGCAG	473	0.11715547497504564	No Hit
GTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCA	467	0.11566935901341714	No Hit
ATGCTGTCCTGCCGCCTCCAGTGCGCGCTGGCTGCGCTGTCCA	454	0.11244944109655543	No Hit
GTCCTGCCGCCTCCAGTGCGCGCTGGCTGCGCTGTCCATCGTC	449	0.11121101112853168	No Hit
CGGCAGGACAGCATCTCGGCGCCGCGAAAGCCGAGCTGGAGAG	442	0.10947720917329846	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	442	0.10947720917329846	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	434	0.10749572122446048	No Hit
GTTCCTGCTTCCCCGCGGCAGCAGCCAGGGACTTCTGCAGAAA	430	0.1065049772500415	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	430	0.1065049772500415	No Hit
GTTCGGAGCCATCGCTGCTGCCTGCTGATCCGCGCCTAGAGTT	425	0.10526654728201774	No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC	424	0.105018861288413	No Hit
AGTCTGGGGTCCGAGGGAGCGCCGGTGACACAGCCCAGGGCCA	413	0.10229431535876078	No Hit
GAGCTGGAGAGTGGCTGGTCAAACTCTAGGCGCGGATCAGCAG	408	0.10105588539073704	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	2.4768599360474767E-4	0.0
15	0.0	0.0	0.0	2.4768599360474767E-4	0.0
16	0.0	0.0	0.0	0.001486115961628486	0.0
17	0.0	0.0	0.0	0.0019814879488379814	0.0
18	0.0	0.0	0.0	0.0024768599360474766	0.0
19	0.0	0.0	0.0	0.002972231923256972	0.0
20	0.0	0.0	0.0	0.003962975897675963	0.0
21	0.0	0.0	0.0	0.007925951795351925	0.0
22	0.0	0.0	0.0	0.012384299680237382	0.0
23	0.0	0.0	0.0	0.014861159616284859	0.0
24	0.0	0.0	0.0	0.017338019552332336	0.0
25	0.0	0.0	0.0	0.0208056234627988	0.0
26	0.0	0.0	0.0	0.025759343334893756	0.0
27	0.0	0.0	0.0	0.05894926647792994	0.0
28	0.0	0.0	0.0	0.21920210434020165	0.0
29	0.0	0.0	0.0	0.4819969435548389	0.0
30	0.0	0.0	0.0	0.7755048459764649	0.0
31	0.0	0.0	0.0	1.7090333558727588	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	415	0.0	29.86747	1
CTACCGT	25	0.0054921736	29.599998	8
GGACGTA	45	3.999794E-6	28.777777	27
CTGTCGC	35	8.859389E-4	26.428572	9
TTATGCG	35	8.859389E-4	26.428572	37
TACGGCT	50	9.072028E-6	25.899998	30
TATACAC	175	0.0	25.371428	37
ACACGAA	45	1.3211358E-4	24.666666	17
CACGAAA	45	1.3211358E-4	24.666666	18
AAACACG	45	1.3211358E-4	24.666666	15
ATACGGC	45	1.3211358E-4	24.666666	29
TTAGCTA	40	0.0019290292	23.125	4
AGCGACG	40	0.0019290292	23.125	29
TAGCTAC	40	0.0019290292	23.125	5
GACGTAT	50	2.6978969E-4	22.199999	28
TAAACCA	50	2.6978969E-4	22.199999	5
ACGTATA	50	2.6978969E-4	22.199999	29
TATTAGC	60	3.7185815E-5	21.583334	2
TAATACT	45	0.0038215744	20.555555	4
GCGACGC	45	0.0038215744	20.555555	30
>>END_MODULE