##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632729.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2693
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.831043445971034	34.0	31.0	34.0	31.0	34.0
2	32.955811362792424	34.0	33.0	34.0	31.0	34.0
3	33.09617526921649	34.0	33.0	34.0	31.0	34.0
4	36.42666171555886	37.0	37.0	37.0	35.0	37.0
5	36.37133308577794	37.0	37.0	37.0	35.0	37.0
6	36.410694392870404	37.0	37.0	37.0	35.0	37.0
7	36.3754177497215	37.0	37.0	37.0	35.0	37.0
8	36.34831043445971	37.0	37.0	37.0	35.0	37.0
9	38.09877460081693	39.0	39.0	39.0	37.0	39.0
10	38.12699591533605	39.0	39.0	39.0	37.0	39.0
11	38.1867805421463	39.0	39.0	39.0	37.0	39.0
12	38.164129223913854	39.0	39.0	39.0	37.0	39.0
13	38.19160787226142	39.0	39.0	39.0	37.0	39.0
14	39.69922020051987	41.0	40.0	41.0	38.0	41.0
15	39.78165614556257	41.0	40.0	41.0	38.0	41.0
16	39.70813219457854	41.0	40.0	41.0	37.0	41.0
17	39.74675083549944	41.0	40.0	41.0	37.0	41.0
18	39.68659487560342	41.0	40.0	41.0	37.0	41.0
19	39.6784255477163	41.0	40.0	41.0	37.0	41.0
20	39.623839584106946	41.0	40.0	41.0	37.0	41.0
21	39.64017823988117	41.0	40.0	41.0	37.0	41.0
22	39.64611956925362	41.0	40.0	41.0	37.0	41.0
23	39.582621611585594	41.0	40.0	41.0	37.0	41.0
24	39.54771630152246	41.0	40.0	41.0	37.0	41.0
25	39.4444857036762	41.0	39.0	41.0	37.0	41.0
26	39.352766431489044	41.0	39.0	41.0	36.0	41.0
27	39.26216115855923	41.0	39.0	41.0	36.0	41.0
28	39.254363163757894	41.0	39.0	41.0	36.0	41.0
29	39.159673226884514	41.0	39.0	41.0	35.0	41.0
30	39.13553657630895	41.0	39.0	41.0	35.0	41.0
31	39.0185666542889	41.0	39.0	41.0	35.0	41.0
32	38.95915336056443	40.0	39.0	41.0	35.0	41.0
33	38.9209060527293	40.0	39.0	41.0	35.0	41.0
34	38.893798737467506	40.0	39.0	41.0	35.0	41.0
35	38.751949498700334	40.0	39.0	41.0	35.0	41.0
36	38.728184181210544	40.0	38.0	41.0	35.0	41.0
37	38.57222428518381	40.0	38.0	41.0	35.0	41.0
38	38.576308949127366	40.0	38.0	41.0	35.0	41.0
39	38.54920163386558	40.0	38.0	41.0	34.0	41.0
40	38.37950241366506	40.0	38.0	41.0	35.0	41.0
41	38.3821017452655	40.0	38.0	41.0	35.0	41.0
42	38.31080579279614	40.0	38.0	41.0	35.0	41.0
43	37.50835499443	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	2.0
21	0.0
22	0.0
23	1.0
24	1.0
25	7.0
26	4.0
27	5.0
28	9.0
29	15.0
30	25.0
31	28.0
32	27.0
33	31.0
34	55.0
35	79.0
36	129.0
37	233.0
38	531.0
39	1511.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.25770516152989	16.412922391385074	13.739324173783885	27.59004827330115
2	20.64611956925362	21.834385443743038	30.523579650946896	26.99591533605644
3	20.757519494987005	20.20051986632009	28.889714073523955	30.152246565168955
4	16.190122539918306	15.484589676940216	31.48904567396955	36.83624210917193
5	18.269587820274786	31.637578908280727	33.1229112513925	16.969922020051985
6	34.31117712588191	33.34571110285927	15.336056442629037	17.00705532862978
7	30.746379502413667	27.961381359079095	19.197920534719643	22.094318603787595
8	28.33271444485704	30.634979576680283	20.1262532491645	20.90605272929818
9	27.627181581878947	13.739324173783885	17.41552172298552	41.217972521351655
10	20.01485332343112	24.396583735610843	27.701448199034534	27.887114741923508
11	40.215373189751205	19.754920163386558	18.863720757519495	21.16598588934274
12	23.50538432974378	22.46565168956554	26.067582621611585	27.961381359079095
13	33.642777571481616	16.784255477163015	22.948384701076865	26.6245822502785
14	23.87671741552172	18.23245451169699	24.656516895655404	33.23431117712588
15	28.70404753063498	23.468251021165987	19.754920163386558	28.072781284812475
16	25.547716301522467	22.391385072409953	22.7255848496101	29.335313776457483
17	27.181581878945416	23.096917935388042	20.720386186409208	29.001113999257335
18	27.255848496101002	20.683252877831414	24.21091719272187	27.84998143334571
19	27.812848124767918	21.68585220943186	23.87671741552172	26.6245822502785
20	27.701448199034534	21.760118826587448	23.134051243965835	27.40438173041218
21	28.147047901968065	23.096917935388042	21.203119197920532	27.552914964723357
22	27.887114741923508	22.391385072409953	21.16598588934274	28.555514296323803
23	27.84998143334571	20.943186037875975	22.83698477534348	28.36984775343483
24	29.669513553657627	20.83178611214259	22.317118455254363	27.181581878945416
25	29.780913479391014	22.20571852952098	22.02005198663201	25.993316004455995
26	29.261047159301896	22.242851838098773	20.83178611214259	27.664314890456737
27	26.17898254734497	21.722985518009654	24.879316747122168	27.218715187523205
28	26.401782398811736	23.913850724099518	22.242851838098773	27.441515038989973
29	27.255848496101002	23.653917564054957	22.057185295209802	27.03304864463424
30	26.921648718900855	22.057185295209802	23.17118455254363	27.84998143334571
31	28.70404753063498	22.242851838098773	21.982918678054215	27.070181953212032
32	25.584849610100257	22.242851838098773	22.98551800965466	29.186780542146305
33	25.993316004455995	21.4259190493873	24.879316747122168	27.701448199034534
34	25.733382844411434	21.908652060898625	24.80505012996658	27.552914964723357
35	28.147047901968065	22.614184923876717	23.31971778685481	25.919049387300408
36	26.438915707389526	22.168585220943186	24.136650575566282	27.255848496101002
37	26.773115484589677	21.017452655031562	24.248050501299666	27.961381359079095
38	26.95878202747865	20.868919420720385	25.547716301522467	26.6245822502785
39	25.02784998143335	20.46045302636465	28.035647976234685	26.476049015967323
40	24.322317118455256	21.16598588934274	27.218715187523205	27.292981804678796
41	22.948384701076865	20.274786483475676	28.666914222057184	28.109914593390272
42	23.3939844040104	19.68065354623097	28.518380987746006	28.406981062012626
43	22.46565168956554	20.349053100631266	29.372447085035276	27.812848124767918
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	1.0
8	0.5
9	0.0
10	0.0
11	0.0
12	0.0
13	0.5
14	1.0
15	6.5
16	12.0
17	9.0
18	6.0
19	6.0
20	5.0
21	4.0
22	3.0
23	2.0
24	1.5
25	1.0
26	1.0
27	3.0
28	5.0
29	6.5
30	8.0
31	14.5
32	21.0
33	21.0
34	24.0
35	27.0
36	32.0
37	37.0
38	47.0
39	57.0
40	57.0
41	74.5
42	92.0
43	100.5
44	109.0
45	144.0
46	179.0
47	179.0
48	210.0
49	241.0
50	272.5
51	304.0
52	307.5
53	311.0
54	311.0
55	289.5
56	268.0
57	266.5
58	265.0
59	233.5
60	202.0
61	202.0
62	176.0
63	150.0
64	139.0
65	128.0
66	102.5
67	77.0
68	77.0
69	73.5
70	70.0
71	51.5
72	33.0
73	30.5
74	28.0
75	28.0
76	24.0
77	20.0
78	19.0
79	18.0
80	12.5
81	7.0
82	7.0
83	6.5
84	6.0
85	3.5
86	1.0
87	1.0
88	1.0
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2693.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	90.3082064611957
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.58552631578947	84.51541032305978
2	4.194078947368421	7.575194949870033
3	1.3980263157894737	3.7875974749350165
4	0.3289473684210526	1.188265874489417
5	0.12335526315789473	0.5569996286669142
6	0.1644736842105263	0.8911994058670627
7	0.12335526315789473	0.77979948013368
8	0.041118421052631575	0.29706646862235425
9	0.0	0.0
>10	0.041118421052631575	0.40846639435573706
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT	11	0.40846639435573706	TruSeq Adapter, Index 11 (95% over 21bp)
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	8	0.29706646862235425	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7	0.25993316004455996	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	7	0.25993316004455996	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	7	0.25993316004455996	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	6	0.22279985146676567	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	6	0.22279985146676567	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	6	0.22279985146676567	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	6	0.22279985146676567	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	5	0.18566654288897141	No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG	5	0.18566654288897141	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	5	0.18566654288897141	No Hit
CCTGTGAATGGCGCTTGTCCTCGTTCATCTGATCAGGATCACT	4	0.14853323431117713	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	4	0.14853323431117713	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	4	0.14853323431117713	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	4	0.14853323431117713	No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA	4	0.14853323431117713	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	4	0.14853323431117713	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	4	0.14853323431117713	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	4	0.14853323431117713	No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCG	3	0.11139992573338284	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGCTGTCT	3	0.11139992573338284	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	3	0.11139992573338284	No Hit
GAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACATGCTG	3	0.11139992573338284	No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC	3	0.11139992573338284	No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC	3	0.11139992573338284	No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC	3	0.11139992573338284	No Hit
CTTCATACACGCGGCATGGCTGCATCAGGCTTGCGCCCATTGT	3	0.11139992573338284	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	3	0.11139992573338284	No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG	3	0.11139992573338284	No Hit
GCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGG	3	0.11139992573338284	No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC	3	0.11139992573338284	No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC	3	0.11139992573338284	No Hit
CTCCCCAGGCGGTCGACTTAACGCGTTAGCTCCGGAAGCCACG	3	0.11139992573338284	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	3	0.11139992573338284	No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA	3	0.11139992573338284	No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG	3	0.11139992573338284	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	3	0.11139992573338284	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	3	0.11139992573338284	No Hit
GCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATC	3	0.11139992573338284	No Hit
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC	3	0.11139992573338284	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGCTGTCTCTTATA	3	0.11139992573338284	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	3	0.11139992573338284	No Hit
CCTTACCGACGCTTATCGCAGATTAGCACGTCCTTCATCGCCT	3	0.11139992573338284	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	3	0.11139992573338284	No Hit
GTTGTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAAT	3	0.11139992573338284	No Hit
CTACTGGGGGAATCTCGGTTGATTTCTTTTCCTCGGGGTACTT	3	0.11139992573338284	No Hit
CTTCCTCCCCGCTGAAAGTACTTTACAACCCGAAGGCCTTCTT	3	0.11139992573338284	No Hit
CGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGG	3	0.11139992573338284	No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG	3	0.11139992573338284	No Hit
ATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAGGGCAC	3	0.11139992573338284	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	3	0.11139992573338284	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	3	0.11139992573338284	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	3	0.11139992573338284	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.03713330857779428	0.0
29	0.0	0.0	0.0	0.25993316004455996	0.0
30	0.0	0.0	0.0	0.33419977720014854	0.0
31	0.0	0.0	0.0	0.5941329372447085	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE