FastQCFastQC Report
Fri 10 Feb 2017
ERR1632728.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632728.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156855
Sequences flagged as poor quality0
Sequence length43
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA3610.23014886360014025No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA2610.16639571578846707No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA2610.16639571578846707No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA2570.16384558987600015No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC2560.1632080583978834No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG2510.16002040100729975No Hit
GATCAGCAGGCAGCAGCGATGGCTCCGAACCTCGCTCCTAAAG2370.15109496031366548No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC2320.14790730292308182No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC2210.14089445666379777No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA2200.14025692518568103No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT2140.13643173631698066No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA2010.12814382710146313No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT1970.1255937011889962No Hit
CTGCTGATCCGCGCCTAGAGTTTGACCAGCCACTCTCCAGCTC1910.12176851232029581No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA1910.12176851232029581No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG1900.12113098084217909No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA1850.11794332345159543No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA1850.11794332345159543No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA1850.11794332345159543No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG1850.11794332345159543No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT1760.11220554014854482No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT1750.11156800867042811No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC1740.11093047719231137No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC1730.11029294571419464No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC1720.1096554142360779No Hit
GAGCTGGAGAGTGGCTGGTCAAACTCTAGGCGCGGATCAGCAG1700.10838035127984445No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG1700.10838035127984445No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA1690.10774281980172772No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC1680.10710528832361098No Hit
GTCCTGCCGCCTCCAGTGCGCGCTGGCTGCGCTGTCCATCGTC1680.10710528832361098No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT1660.10583022536737752No Hit
GGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGG1660.10583022536737752No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA1610.10264256797679386No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA1610.10264256797679386No Hit
CGGCAGGACAGCATCTCGGCGCCGCGAAAGCCGAGCTGGAGAG1590.10136750502056038No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1700.031.5588251
AATCCTT250.005482062729.623
GCTGACT250.005482062729.68
AATGAGC358.8351493E-426.42857234
CAATGAG358.8351493E-426.42857233
GGTAATA1400.023.78571525
TAACTCC1400.023.7857154
CGTGCCA1450.022.96551710
TCCGTGC1450.022.9655178
CGGTAAT1450.022.96551724
GCGGTAA1550.022.6774223
AGCCGCG1500.022.19999919
CCGTGCC1500.022.1999999
AACTCCG1500.022.1999995
TAATACG1450.021.68965527
ATATCAG603.6999478E-521.5833325
CTCCGTG1550.021.4838717
CGCGGTA1600.020.812522
GACGCTG450.003811316320.55555537
AGCAGTA450.003811316320.55555525
Produced by FastQC (version 0.11.5)