##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632728.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	156855
Sequences flagged as poor quality	0
Sequence length	43
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.01923432469478	34.0	33.0	34.0	31.0	34.0
2	33.154818144145864	34.0	33.0	34.0	31.0	34.0
3	33.23343215071244	34.0	34.0	34.0	31.0	34.0
4	36.530611073921776	37.0	37.0	37.0	35.0	37.0
5	36.49032545981958	37.0	37.0	37.0	35.0	37.0
6	36.53555194287718	37.0	37.0	37.0	35.0	37.0
7	36.51660450734755	37.0	37.0	37.0	35.0	37.0
8	36.500270950878196	37.0	37.0	37.0	35.0	37.0
9	38.29758694335533	39.0	39.0	39.0	37.0	39.0
10	38.3392687513946	39.0	39.0	39.0	37.0	39.0
11	38.403289662427085	39.0	39.0	39.0	37.0	39.0
12	38.31938414459214	39.0	39.0	39.0	37.0	39.0
13	38.36535653947914	39.0	39.0	39.0	37.0	39.0
14	39.92691976666348	41.0	40.0	41.0	38.0	41.0
15	39.92179401357942	41.0	40.0	41.0	38.0	41.0
16	39.90019444710082	41.0	40.0	41.0	38.0	41.0
17	39.86988620063116	41.0	40.0	41.0	38.0	41.0
18	39.884625928405214	41.0	40.0	41.0	38.0	41.0
19	39.85847438717287	41.0	40.0	41.0	38.0	41.0
20	39.85477670459979	41.0	40.0	41.0	38.0	41.0
21	39.82392018105894	41.0	40.0	41.0	38.0	41.0
22	39.79552452902362	41.0	40.0	41.0	38.0	41.0
23	39.77548053935163	41.0	40.0	41.0	38.0	41.0
24	39.75448662777725	41.0	40.0	41.0	38.0	41.0
25	39.709566159829144	41.0	40.0	41.0	38.0	41.0
26	39.626151541232346	41.0	40.0	41.0	37.0	41.0
27	39.54983902330178	41.0	40.0	41.0	37.0	41.0
28	39.50509068885276	41.0	39.0	41.0	37.0	41.0
29	39.45777310254694	41.0	39.0	41.0	37.0	41.0
30	39.418915558955725	41.0	39.0	41.0	37.0	41.0
31	39.330126549998404	41.0	39.0	41.0	36.0	41.0
32	39.279991074559305	41.0	39.0	41.0	36.0	41.0
33	39.21721334990915	41.0	39.0	41.0	36.0	41.0
34	39.15691562270887	41.0	39.0	41.0	35.0	41.0
35	39.0868636638934	41.0	39.0	41.0	35.0	41.0
36	39.01284625928405	41.0	39.0	41.0	35.0	41.0
37	38.940983711070736	40.0	39.0	41.0	35.0	41.0
38	38.871511905900356	40.0	38.0	41.0	35.0	41.0
39	38.7643874916324	40.0	38.0	41.0	35.0	41.0
40	38.65751171464091	40.0	38.0	41.0	35.0	41.0
41	38.60698734500016	40.0	38.0	41.0	35.0	41.0
42	38.53158012176851	40.0	38.0	41.0	35.0	41.0
43	37.76401772337509	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	1.0
18	1.0
19	7.0
20	5.0
21	11.0
22	18.0
23	48.0
24	79.0
25	135.0
26	186.0
27	263.0
28	405.0
29	541.0
30	740.0
31	914.0
32	1330.0
33	1748.0
34	2567.0
35	3922.0
36	6478.0
37	13045.0
38	30743.0
39	93667.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.981033438526026	15.45886328137452	11.433489528545472	28.12661375155398
2	21.51031207165854	18.638870294220776	31.292595071881674	28.55822256223901
3	21.93363297312805	18.25762647030697	27.6395397022728	32.16920085429218
4	15.305855726626502	14.862771349335373	32.986516209237834	36.844856714800294
5	18.112906824774473	31.92120110930477	32.46756558605081	17.498326479869945
6	37.134296005865295	31.808358037678108	14.949475630359249	16.107870326097352
7	32.11054795830544	26.115201938095694	18.665009084823563	23.109241018775304
8	28.504032386599086	29.585285773485065	19.890344585763923	22.020337254151922
9	28.40202735010041	11.628574160849192	16.740939083867264	43.22845940518313
10	19.491887411940965	22.362054126422493	29.119887794459853	29.026170667176693
11	41.08699117018903	18.216186924229383	17.497051416913713	23.199770488667877
12	23.466896177998787	22.383092665200344	25.4980714672787	28.65193968952217
13	36.24940231423926	16.40623505785598	19.989799496350134	27.35456313155462
14	25.346338975486916	18.539415383634566	22.094290905613466	34.01995473526505
15	29.564247234707214	23.007873513754742	19.51037582480635	27.91750342673169
16	28.1387268496382	22.070702240923147	21.620605017372732	28.169965892065925
17	27.94172962290013	22.481910044308435	20.72997354244366	28.846386790347772
18	27.152465652991616	20.014663223996685	22.93710751968378	29.895763603327914
19	30.030282745210545	21.52433776417711	21.714322144655892	26.731057345956454
20	31.152338146695996	19.819578591692967	21.43763348315323	27.59044977845781
21	28.06668579261101	21.2878135857958	20.755474801568326	29.890025820024864
22	29.936565617927386	21.395556405597528	19.884606802460873	28.783271174014217
23	28.1310764719008	21.235536004590227	21.29737655796755	29.336010965541426
24	28.75713238341143	20.9562972171751	21.19027126964394	29.09629912976953
25	29.16515252940614	20.896369258232124	22.01459947084887	27.923878741512866
26	29.308597111982404	21.6269803321539	20.70574734627522	28.358675209588473
27	28.666602913518858	20.797551879124033	22.13254279430047	28.403302413056647
28	27.150553058557264	22.439195435274616	20.925695706225493	29.484555799942626
29	28.799209460967134	22.84402792387874	21.329890663351502	27.026871951802622
30	28.48618150521182	20.31430301871155	22.224984858627394	28.974530617449233
31	27.747920053552644	20.854929712154537	21.883267986356827	29.513882247935992
32	26.12221478435498	21.51987504383029	22.01906219119569	30.338847980619043
33	27.27040897644321	20.530426189793122	23.310063434382073	28.889101399381595
34	27.803385292148803	20.70574734627522	23.315163686207008	28.175703675368975
35	27.59491249880463	21.425520385069014	23.092027668866148	27.887539447260206
36	26.76357145134041	20.755474801568326	23.37891683401868	29.10203691307258
37	26.52003442669982	20.552739791527205	24.922380542539287	28.00484523923369
38	26.288610500143445	19.823403780561666	24.534123872366198	29.35386184692869
39	25.375665423480285	19.694622421982086	26.95036817442861	27.979343980109018
40	26.00682158681585	19.767301010487394	26.947180517038028	27.278696885658732
41	23.400592904274646	19.421758949348124	28.10621274425425	29.07143540212298
42	21.61869242293838	20.527876063880655	29.48328073698639	28.370150776194574
43	21.89410602148481	20.233974052468838	28.16486564024099	29.707054285805363
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	2.5
4	5.0
5	5.0
6	8.0
7	11.0
8	9.5
9	8.0
10	14.0
11	20.0
12	20.0
13	35.5
14	51.0
15	83.5
16	116.0
17	113.0
18	110.0
19	110.0
20	139.5
21	169.0
22	142.0
23	115.0
24	130.0
25	145.0
26	145.0
27	160.5
28	176.0
29	235.0
30	294.0
31	435.0
32	576.0
33	576.0
34	934.5
35	1293.0
36	1452.5
37	1612.0
38	2100.5
39	2589.0
40	2589.0
41	3089.0
42	3589.0
43	4632.0
44	5675.0
45	7465.0
46	9255.0
47	9255.0
48	10545.0
49	11835.0
50	13422.0
51	15009.0
52	16471.5
53	17934.0
54	17934.0
55	17125.5
56	16317.0
57	15420.0
58	14523.0
59	14587.0
60	14651.0
61	14651.0
62	13455.0
63	12259.0
64	9906.5
65	7554.0
66	6915.5
67	6277.0
68	6277.0
69	5612.0
70	4947.0
71	4898.0
72	4849.0
73	3911.0
74	2973.0
75	2973.0
76	1914.5
77	856.0
78	668.0
79	480.0
80	376.0
81	272.0
82	272.0
83	219.5
84	167.0
85	120.0
86	73.0
87	52.5
88	32.0
89	32.0
90	28.5
91	25.0
92	18.0
93	11.0
94	6.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	156855.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.468904402154855
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.7075022422091	42.750948328073704
2	6.697573361292523	6.760383793949826
3	2.445586953501004	3.7027828249019796
4	1.3011128936498113	2.626629689840936
5	0.8349860414587623	2.1070415351757994
6	0.6290817679976757	1.904944056612795
7	0.4648636357894471	1.6422810876287017
8	0.31706731680204137	1.280163208058398
9	0.28169725755719216	1.2795256765802812
>10	1.9946186981291765	20.079053903286475
>50	0.224852519485113	7.768958592330496
>100	0.10105731212814066	8.097287303560613
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	361	0.23014886360014025	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	261	0.16639571578846707	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	261	0.16639571578846707	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	257	0.16384558987600015	No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC	256	0.1632080583978834	No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG	251	0.16002040100729975	No Hit
GATCAGCAGGCAGCAGCGATGGCTCCGAACCTCGCTCCTAAAG	237	0.15109496031366548	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	232	0.14790730292308182	No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC	221	0.14089445666379777	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	220	0.14025692518568103	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	214	0.13643173631698066	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	201	0.12814382710146313	No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT	197	0.1255937011889962	No Hit
CTGCTGATCCGCGCCTAGAGTTTGACCAGCCACTCTCCAGCTC	191	0.12176851232029581	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	191	0.12176851232029581	No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG	190	0.12113098084217909	No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA	185	0.11794332345159543	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	185	0.11794332345159543	No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA	185	0.11794332345159543	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	185	0.11794332345159543	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	176	0.11220554014854482	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	175	0.11156800867042811	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	174	0.11093047719231137	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	173	0.11029294571419464	No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC	172	0.1096554142360779	No Hit
GAGCTGGAGAGTGGCTGGTCAAACTCTAGGCGCGGATCAGCAG	170	0.10838035127984445	No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG	170	0.10838035127984445	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	169	0.10774281980172772	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	168	0.10710528832361098	No Hit
GTCCTGCCGCCTCCAGTGCGCGCTGGCTGCGCTGTCCATCGTC	168	0.10710528832361098	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	166	0.10583022536737752	No Hit
GGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGG	166	0.10583022536737752	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	161	0.10264256797679386	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	161	0.10264256797679386	No Hit
CGGCAGGACAGCATCTCGGCGCCGCGAAAGCCGAGCTGGAGAG	159	0.10136750502056038	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.001275062956233464	0.0
17	0.0	0.0	0.0	0.001275062956233464	0.0
18	0.0	0.0	0.0	0.00318765739058366	0.0
19	0.0	0.0	0.0	0.005737783303050588	0.0
20	0.0	0.0	0.0	0.007012846259284052	0.0
21	0.0	0.0	0.0	0.007650377737400784	0.0
22	0.0	0.0	0.0	0.010838035127984444	0.0
23	0.0	0.0	0.0	0.013388161040451372	0.0
24	0.0	0.0	0.0	0.014663223996684837	6.37531478116732E-4
25	0.0	0.0	0.0	0.016575818431035033	6.37531478116732E-4
26	0.0	0.0	0.0	0.024226196168435816	6.37531478116732E-4
27	0.0	0.0	0.0	0.04653979790252144	6.37531478116732E-4
28	0.0	0.0	0.0	0.16193299544164994	6.37531478116732E-4
29	0.0	0.0	0.0	0.354467501832903	6.37531478116732E-4
30	0.0	0.0	0.0	0.5527397915272066	6.37531478116732E-4
31	6.37531478116732E-4	0.0	0.0	1.232348347199643	6.37531478116732E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	170	0.0	31.558825	1
AATCCTT	25	0.0054820627	29.6	23
GCTGACT	25	0.0054820627	29.6	8
AATGAGC	35	8.8351493E-4	26.428572	34
CAATGAG	35	8.8351493E-4	26.428572	33
GGTAATA	140	0.0	23.785715	25
TAACTCC	140	0.0	23.785715	4
CGTGCCA	145	0.0	22.965517	10
TCCGTGC	145	0.0	22.965517	8
CGGTAAT	145	0.0	22.965517	24
GCGGTAA	155	0.0	22.67742	23
AGCCGCG	150	0.0	22.199999	19
CCGTGCC	150	0.0	22.199999	9
AACTCCG	150	0.0	22.199999	5
TAATACG	145	0.0	21.689655	27
ATATCAG	60	3.6999478E-5	21.583332	5
CTCCGTG	155	0.0	21.483871	7
CGCGGTA	160	0.0	20.8125	22
GACGCTG	45	0.0038113163	20.555555	37
AGCAGTA	45	0.0038113163	20.555555	25
>>END_MODULE