FastQCFastQC Report
Fri 10 Feb 2017
ERR1632727.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632727.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44334
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT600.13533631073216945TruSeq Adapter, Index 1 (95% over 22bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA550.124058284837822No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA500.11278025894347454No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC470.10601344340686607No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA470.10601344340686607No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT460.10375783822799657No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA450.10150223304912709No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTAA200.001822901437.023
GGGTGAG200.001822901437.01
CGGTAAT200.001822901437.024
TTCGCAG250.00544019229.615
CGCGGTA250.00544019229.622
AGCCGCG250.00544019229.619
GTGCCAG250.00544019229.611
CCAGCAG701.1859747E-418.514
TCTTATA1451.4006218E-1017.86206837
CTTATAC752.0113317E-417.26666637
GTATCAA650.0015477617.0769231
ATCAACG750.00402218514.83
AACGCAG800.00617348313.8756
TCAACGC800.00617348313.8754
CAACGCA800.00617348313.8755
CTCTTAT1906.4483174E-913.63157936
ACGCAGA850.00921942613.0588237
TCCTGTC1050.00257505212.33333337
GGCTGTC1401.340996E-411.89285737
TCTCTTA2708.434454E-79.59259235
Produced by FastQC (version 0.11.5)