FastQCFastQC Report
Fri 10 Feb 2017
ERR1632726.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632726.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1818
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT70.385038503850385RNA PCR Primer, Index 29 (95% over 23bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA50.27502750275027504No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA50.27502750275027504No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA40.22002200220022No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC40.22002200220022No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG40.22002200220022No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA40.22002200220022No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA40.22002200220022No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT40.22002200220022No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT30.16501650165016502No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG30.16501650165016502No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC30.16501650165016502No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA30.16501650165016502No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT20.11001100110011No Hit
GGCGATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGT20.11001100110011No Hit
CCTCAAGGGCACAACCTCCAAGTCGACATCGTTTACGGCGTGG20.11001100110011No Hit
TTACAACCCGAAGGCCTTCTTCATACACGCGGCATGGCTGCAT20.11001100110011No Hit
ATCAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATC20.11001100110011No Hit
CACTAGGCCAGTCCTGGTCAACAGCCATTTTCTCTGTCCTAGG20.11001100110011No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC20.11001100110011No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT20.11001100110011No Hit
TTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGG20.11001100110011No Hit
GGATCACTGAGTGGGTCTGCCTGGGAAGCTGAGAATGATCTGG20.11001100110011No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC20.11001100110011No Hit
CTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCCTGTC20.11001100110011No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.11001100110011No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA20.11001100110011No Hit
CTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAG20.11001100110011No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC20.11001100110011No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCCT20.11001100110011No Hit
CTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTC20.11001100110011RNA PCR Primer, Index 29 (95% over 24bp)
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT20.11001100110011No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC20.11001100110011No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA20.11001100110011No Hit
GTTTCTATCTACTTCAAATTCCTCCCTGTACGAAAGGACAAGA20.11001100110011No Hit
CGTCAGCAAAGAAGCAAGCTTCTTCCTGTTACCGTTCGACTTG20.11001100110011No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG20.11001100110011No Hit
CGTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCCGTACACA20.11001100110011No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTG20.11001100110011No Hit
CATCAAATCGTACCCCAAACCGACACAGGTGGTCAGGTAGAGA20.11001100110011No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT20.11001100110011No Hit
ATTCACCAGTGACTACAGCAAGTATCTGGACTCCAGGCGCTGT20.11001100110011No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA20.11001100110011No Hit
CTTCCGGAGCTAACGCGTTAAGTCGACCGCCTGGGGAGTACGG20.11001100110011No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTC20.11001100110011No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCTGTCT20.11001100110011No Hit
AACCCAGTGTGTTTCGACACACTATCATTAACTGAATCCATAG20.11001100110011No Hit
CGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACG20.11001100110011No Hit
CTCCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGA20.11001100110011No Hit
AGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAATA20.11001100110011No Hit
CAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTT20.11001100110011No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG20.11001100110011No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG20.11001100110011No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC20.11001100110011No Hit
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCC20.11001100110011No Hit
GCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTC20.11001100110011No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT20.11001100110011No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC20.11001100110011No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGC20.11001100110011No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA20.11001100110011No Hit
ATAGTGAACCAGTACCGTGAGGGAAAAAAAAAAAAAAAAAAAA20.11001100110011No Hit
GTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTT20.11001100110011No Hit
TTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTAT20.11001100110011No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT20.11001100110011No Hit
CTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACC20.11001100110011No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)