##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632726.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1818
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.52200220022002	34.0	31.0	34.0	31.0	34.0
2	32.685918591859185	34.0	31.0	34.0	31.0	34.0
3	32.743674367436746	34.0	31.0	34.0	31.0	34.0
4	36.21892189218922	37.0	37.0	37.0	35.0	37.0
5	36.12926292629263	37.0	35.0	37.0	35.0	37.0
6	36.16226622662266	37.0	37.0	37.0	35.0	37.0
7	36.24312431243124	37.0	37.0	37.0	35.0	37.0
8	36.18701870187019	37.0	37.0	37.0	35.0	37.0
9	37.84983498349835	39.0	38.0	39.0	35.0	39.0
10	37.87073707370737	39.0	38.0	39.0	35.0	39.0
11	38.01045104510451	39.0	38.0	39.0	35.0	39.0
12	37.955995599559955	39.0	38.0	39.0	35.0	39.0
13	37.96589658965897	39.0	38.0	39.0	35.0	39.0
14	39.46699669966997	41.0	39.0	41.0	36.0	41.0
15	39.40924092409241	41.0	39.0	41.0	36.0	41.0
16	39.34158415841584	41.0	39.0	41.0	36.0	41.0
17	39.32838283828383	41.0	39.0	41.0	37.0	41.0
18	39.3943894389439	41.0	39.0	41.0	36.0	41.0
19	39.32893289328933	41.0	39.0	41.0	36.0	41.0
20	39.31518151815182	41.0	39.0	41.0	36.0	41.0
21	39.34048404840484	41.0	39.0	41.0	36.0	41.0
22	39.317381738173815	41.0	39.0	41.0	36.0	41.0
23	39.2002200220022	40.0	39.0	41.0	36.0	41.0
24	39.175467546754675	40.0	39.0	41.0	36.0	41.0
25	39.103960396039604	40.0	39.0	41.0	35.0	41.0
26	39.00990099009901	40.0	39.0	41.0	35.0	41.0
27	38.98239823982398	40.0	39.0	41.0	35.0	41.0
28	38.94994499449945	40.0	38.0	41.0	35.0	41.0
29	38.98514851485149	40.0	38.0	41.0	35.0	41.0
30	38.82233223322332	40.0	38.0	41.0	35.0	41.0
31	38.77887788778878	40.0	38.0	41.0	35.0	41.0
32	38.675467546754675	40.0	38.0	41.0	35.0	41.0
33	38.625962596259626	40.0	38.0	41.0	35.0	41.0
34	38.50880088008801	40.0	38.0	41.0	35.0	41.0
35	38.45049504950495	40.0	38.0	41.0	34.0	41.0
36	38.36853685368537	40.0	38.0	41.0	34.0	41.0
37	38.156215621562154	40.0	38.0	41.0	33.0	41.0
38	38.238173817381735	40.0	38.0	41.0	34.0	41.0
39	37.90649064906491	40.0	37.0	41.0	33.0	41.0
40	37.89273927392739	40.0	37.0	41.0	33.0	41.0
41	37.76842684268427	40.0	37.0	41.0	33.0	41.0
42	37.84763476347635	40.0	37.0	41.0	33.0	41.0
43	37.02530253025302	39.0	36.0	41.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	1.0
23	1.0
24	1.0
25	4.0
26	7.0
27	7.0
28	11.0
29	10.0
30	22.0
31	23.0
32	23.0
33	34.0
34	49.0
35	77.0
36	89.0
37	183.0
38	370.0
39	906.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	44.224422442244226	16.006600660066006	11.991199119911991	27.77777777777778
2	21.3971397139714	18.206820682068205	31.903190319031903	28.492849284928496
3	19.58195819581958	19.966996699669966	28.437843784378437	32.01320132013201
4	16.776677667766776	15.126512651265125	33.4983498349835	34.5984598459846
5	17.546754675467547	31.02310231023102	32.78327832783278	18.646864686468646
6	36.13861386138614	32.72827282728273	14.356435643564355	16.776677667766776
7	30.528052805280524	27.997799779978	18.866886688668867	22.607260726072607
8	26.567656765676567	30.47304730473047	21.177117711771174	21.782178217821784
9	27.22772277227723	13.476347634763478	17.436743674367435	41.859185918591855
10	19.086908690869087	23.87238723872387	30.08800880088009	26.952695269526956
11	40.15401540154016	19.196919691969196	18.096809680968097	22.55225522552255
12	22.22222222222222	24.477447744774476	26.512651265126514	26.787678767876788
13	34.15841584158416	17.876787678767876	21.61716171617162	26.347634763476346
14	24.147414741474147	17.656765676567655	23.32233223322332	34.87348734873487
15	28.877887788778878	23.432343234323433	20.242024202420243	27.447744774477446
16	25.192519251925194	24.697469746974697	21.61716171617162	28.492849284928496
17	26.67766776677668	24.422442244224424	20.407040704070408	28.492849284928496
18	25.467546754675467	21.232123212321234	23.32233223322332	29.977997799779978
19	27.39273927392739	23.817381738173818	21.892189218921892	26.897689768976896
20	29.92299229922992	19.856985698569858	24.972497249724974	25.247524752475247
21	26.567656765676567	23.1023102310231	20.242024202420243	30.08800880088009
22	25.247524752475247	23.597359735973598	22.827282728272827	28.32783278327833
23	27.447744774477446	23.157315731573156	22.662266226622663	26.732673267326735
24	27.77777777777778	20.07700770077008	24.147414741474147	27.997799779978
25	27.77777777777778	23.597359735973598	22.497249724972498	26.127612761276126
26	28.38283828382838	23.54235423542354	19.856985698569858	28.217821782178216
27	25.302530253025303	22.992299229922992	24.367436743674368	27.337733773377337
28	27.77777777777778	22.497249724972498	22.33223322332233	27.39273927392739
29	27.77777777777778	23.817381738173818	21.507150715071507	26.897689768976896
30	25.63256325632563	22.882288228822883	24.257425742574256	27.22772277227723
31	27.887788778877887	23.1023102310231	23.1023102310231	25.90759075907591
32	26.45764576457646	22.937293729372936	22.112211221122113	28.492849284928496
33	26.292629262926294	21.3971397139714	24.58745874587459	27.722772277227726
34	27.117711771177117	21.67216721672167	24.697469746974697	26.512651265126514
35	25.85258525852585	22.277227722772277	26.567656765676567	25.302530253025303
36	27.61276127612761	22.55225522552255	25.027502750275026	24.80748074807481
37	25.192519251925194	23.157315731573156	23.377337733773377	28.272827282728276
38	25.85258525852585	20.9020902090209	25.962596259625965	27.282728272827285
39	25.577557755775576	21.342134213421343	25.687568756875688	27.39273927392739
40	25.797579757975797	20.84708470847085	28.987898789878987	24.367436743674368
41	22.607260726072607	22.497249724972498	27.282728272827285	27.61276127612761
42	23.87238723872387	20.9020902090209	28.877887788778878	26.347634763476346
43	23.26732673267327	20.407040704070408	28.76787678767877	27.557755775577558
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	1.5
4	2.0
5	2.0
6	2.0
7	2.0
8	1.0
9	0.0
10	1.0
11	2.0
12	2.0
13	2.0
14	2.0
15	2.5
16	3.0
17	4.5
18	6.0
19	6.0
20	5.5
21	5.0
22	4.5
23	4.0
24	5.0
25	6.0
26	6.0
27	10.5
28	15.0
29	12.0
30	9.0
31	15.0
32	21.0
33	21.0
34	20.0
35	19.0
36	21.5
37	24.0
38	35.5
39	47.0
40	47.0
41	59.0
42	71.0
43	72.0
44	73.0
45	92.5
46	112.0
47	112.0
48	128.0
49	144.0
50	159.0
51	174.0
52	180.0
53	186.0
54	186.0
55	193.0
56	200.0
57	179.5
58	159.0
59	147.5
60	136.0
61	136.0
62	127.5
63	119.0
64	101.0
65	83.0
66	65.0
67	47.0
68	47.0
69	54.0
70	61.0
71	43.0
72	25.0
73	24.0
74	23.0
75	23.0
76	21.0
77	19.0
78	14.0
79	9.0
80	6.0
81	3.0
82	3.0
83	2.5
84	2.0
85	2.5
86	3.0
87	2.0
88	1.0
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1818.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	94.93949394939494
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.23406720741599	91.36413641364136
2	3.0127462340672073	5.720572057205721
3	0.2317497103128621	0.6600660066006601
4	0.34762456546929316	1.3201320132013201
5	0.11587485515643105	0.5500550055005501
6	0.0	0.0
7	0.057937427578215524	0.385038503850385
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT	7	0.385038503850385	RNA PCR Primer, Index 29 (95% over 23bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	5	0.27502750275027504	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	5	0.27502750275027504	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	4	0.22002200220022	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	4	0.22002200220022	No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG	4	0.22002200220022	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	4	0.22002200220022	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	4	0.22002200220022	No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT	4	0.22002200220022	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	3	0.16501650165016502	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	3	0.16501650165016502	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	3	0.16501650165016502	No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA	3	0.16501650165016502	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2	0.11001100110011	No Hit
GGCGATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGT	2	0.11001100110011	No Hit
CCTCAAGGGCACAACCTCCAAGTCGACATCGTTTACGGCGTGG	2	0.11001100110011	No Hit
TTACAACCCGAAGGCCTTCTTCATACACGCGGCATGGCTGCAT	2	0.11001100110011	No Hit
ATCAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATC	2	0.11001100110011	No Hit
CACTAGGCCAGTCCTGGTCAACAGCCATTTTCTCTGTCCTAGG	2	0.11001100110011	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	2	0.11001100110011	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2	0.11001100110011	No Hit
TTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGG	2	0.11001100110011	No Hit
GGATCACTGAGTGGGTCTGCCTGGGAAGCTGAGAATGATCTGG	2	0.11001100110011	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	2	0.11001100110011	No Hit
CTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCCTGTC	2	0.11001100110011	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2	0.11001100110011	No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA	2	0.11001100110011	No Hit
CTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAG	2	0.11001100110011	No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC	2	0.11001100110011	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCCT	2	0.11001100110011	No Hit
CTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTC	2	0.11001100110011	RNA PCR Primer, Index 29 (95% over 24bp)
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	2	0.11001100110011	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	2	0.11001100110011	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	2	0.11001100110011	No Hit
GTTTCTATCTACTTCAAATTCCTCCCTGTACGAAAGGACAAGA	2	0.11001100110011	No Hit
CGTCAGCAAAGAAGCAAGCTTCTTCCTGTTACCGTTCGACTTG	2	0.11001100110011	No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG	2	0.11001100110011	No Hit
CGTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCCGTACACA	2	0.11001100110011	No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTG	2	0.11001100110011	No Hit
CATCAAATCGTACCCCAAACCGACACAGGTGGTCAGGTAGAGA	2	0.11001100110011	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	2	0.11001100110011	No Hit
ATTCACCAGTGACTACAGCAAGTATCTGGACTCCAGGCGCTGT	2	0.11001100110011	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	2	0.11001100110011	No Hit
CTTCCGGAGCTAACGCGTTAAGTCGACCGCCTGGGGAGTACGG	2	0.11001100110011	No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTC	2	0.11001100110011	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCTGTCT	2	0.11001100110011	No Hit
AACCCAGTGTGTTTCGACACACTATCATTAACTGAATCCATAG	2	0.11001100110011	No Hit
CGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACG	2	0.11001100110011	No Hit
CTCCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGA	2	0.11001100110011	No Hit
AGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAATA	2	0.11001100110011	No Hit
CAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTT	2	0.11001100110011	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	2	0.11001100110011	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	2	0.11001100110011	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	2	0.11001100110011	No Hit
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCC	2	0.11001100110011	No Hit
GCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTC	2	0.11001100110011	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	2	0.11001100110011	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	2	0.11001100110011	No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGC	2	0.11001100110011	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	2	0.11001100110011	No Hit
ATAGTGAACCAGTACCGTGAGGGAAAAAAAAAAAAAAAAAAAA	2	0.11001100110011	No Hit
GTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTT	2	0.11001100110011	No Hit
TTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTAT	2	0.11001100110011	No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT	2	0.11001100110011	No Hit
CTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACC	2	0.11001100110011	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.05500550055005501	0.0
25	0.0	0.0	0.0	0.05500550055005501	0.0
26	0.0	0.0	0.0	0.05500550055005501	0.0
27	0.0	0.0	0.0	0.05500550055005501	0.0
28	0.0	0.0	0.0	0.16501650165016502	0.0
29	0.0	0.0	0.0	0.27502750275027504	0.0
30	0.0	0.0	0.0	0.44004400440044006	0.0
31	0.0	0.0	0.0	0.8800880088008801	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE