##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632725.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2282
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.55740578439965	34.0	31.0	34.0	31.0	34.0
2	32.68273444347064	34.0	31.0	34.0	31.0	34.0
3	32.76292725679229	34.0	31.0	34.0	31.0	34.0
4	36.19412795793164	37.0	37.0	37.0	35.0	37.0
5	36.132778264680105	37.0	35.0	37.0	35.0	37.0
6	36.17309377738825	37.0	36.0	37.0	35.0	37.0
7	36.20639789658195	37.0	36.0	37.0	35.0	37.0
8	36.11656441717791	37.0	36.0	37.0	35.0	37.0
9	37.778702892199824	39.0	38.0	39.0	35.0	39.0
10	37.852760736196316	39.0	38.0	39.0	35.0	39.0
11	37.9364592462752	39.0	38.0	39.0	35.0	39.0
12	37.937773882559156	39.0	38.0	39.0	35.0	39.0
13	37.952234881682735	39.0	38.0	39.0	35.0	39.0
14	39.338299737072745	41.0	39.0	41.0	36.0	41.0
15	39.25810692375109	41.0	39.0	41.0	36.0	41.0
16	39.21340929009641	40.0	39.0	41.0	36.0	41.0
17	39.23093777388256	41.0	39.0	41.0	36.0	41.0
18	39.20815074496056	41.0	39.0	41.0	36.0	41.0
19	39.29754601226994	41.0	39.0	41.0	36.0	41.0
20	39.2760736196319	41.0	39.0	41.0	36.0	41.0
21	39.232252410166524	41.0	39.0	41.0	36.0	41.0
22	39.20639789658195	41.0	39.0	41.0	36.0	41.0
23	39.08588957055215	40.0	39.0	41.0	35.0	41.0
24	39.10780017528484	41.0	39.0	41.0	35.0	41.0
25	38.969325153374236	40.0	39.0	41.0	35.0	41.0
26	38.792725679228745	40.0	39.0	41.0	35.0	41.0
27	38.92418930762489	40.0	38.0	41.0	35.0	41.0
28	38.71516213847502	40.0	38.0	41.0	35.0	41.0
29	38.74014022787029	40.0	38.0	41.0	35.0	41.0
30	38.57186678352323	40.0	38.0	41.0	34.0	41.0
31	38.494741454864155	40.0	38.0	41.0	34.0	41.0
32	38.42857142857143	40.0	38.0	41.0	34.0	41.0
33	38.3396143733567	40.0	38.0	41.0	34.0	41.0
34	38.30937773882559	40.0	38.0	41.0	33.0	41.0
35	38.292725679228745	40.0	38.0	41.0	34.0	41.0
36	38.264241893076246	40.0	38.0	41.0	34.0	41.0
37	38.07537248028046	40.0	37.0	41.0	33.0	41.0
38	37.88957055214724	40.0	37.0	41.0	33.0	41.0
39	37.870289219982475	40.0	37.0	41.0	33.0	41.0
40	37.7125328659071	40.0	37.0	41.0	33.0	41.0
41	37.80937773882559	40.0	37.0	41.0	33.0	41.0
42	37.70289219982472	40.0	37.0	41.0	33.0	41.0
43	36.88562664329535	39.0	35.0	41.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	0.0
20	1.0
21	0.0
22	1.0
23	4.0
24	5.0
25	5.0
26	4.0
27	9.0
28	6.0
29	24.0
30	23.0
31	38.0
32	39.0
33	44.0
34	68.0
35	106.0
36	125.0
37	187.0
38	472.0
39	1119.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.813321647677476	16.739702015775634	12.839614373356707	27.607361963190186
2	20.46450482033304	20.02629272567923	30.981595092024538	28.52760736196319
3	21.82296231375986	19.938650306748464	28.48378615249781	29.754601226993866
4	16.301489921121824	15.11831726555653	32.42769500438212	36.15249780893953
5	18.536371603856267	33.96143733567046	32.2524101665206	15.249780893952671
6	33.78615249780894	34.44347063978966	15.11831726555653	16.65205959684487
7	30.017528483786155	28.089395267309374	19.018404907975462	22.87467134092901
8	27.081507449605606	31.68273444347064	19.98247151621385	21.2532865907099
9	27.651183172655564	13.234005258545137	17.747589833479406	41.367221735319895
10	19.71954425942156	22.042068361086766	29.491673970201575	28.7467134092901
11	40.1840490797546	19.237510955302366	16.21384750219106	24.364592462751972
12	23.53198948290973	24.27695004382121	26.029798422436457	26.1612620508326
13	35.45135845749343	15.994741454864153	19.851007887817705	28.702892199824714
14	24.802804557405782	19.67572304995618	22.743207712532865	32.778264680105174
15	28.878177037686243	23.838737949167395	19.456617002629272	27.82646801051709
16	26.380368098159508	22.96231375985977	22.655565293602102	28.001752848378615
17	28.045574057843996	24.01402278702892	21.954425942156004	25.98597721297108
18	25.766871165644172	20.858895705521473	24.101665205959684	29.27256792287467
19	28.92199824715162	20.595968448729185	21.428571428571427	29.053461875547764
20	29.88606485539001	21.51621384750219	20.245398773006134	28.352322524101663
21	28.13321647677476	23.31288343558282	22.261174408413673	26.292725679228745
22	29.71078001752848	21.82296231375986	21.165644171779142	27.300613496932513
23	29.228746713409294	23.22524101665206	21.603856266432953	25.942156003505694
24	28.264680105170903	22.348816827344432	22.348816827344432	27.03768624014023
25	29.36021034180543	22.48028045574058	22.304995617879054	25.854513584574935
26	28.352322524101663	22.304995617879054	21.779141104294478	27.5635407537248
27	28.352322524101663	22.12971078001753	22.304995617879054	27.21297107800175
28	26.248904469763367	23.093777388255916	22.611744084136724	28.045574057843996
29	27.607361963190186	24.01402278702892	22.699386503067483	25.679228746713413
30	28.52760736196319	23.31288343558282	21.034180543382995	27.12532865907099
31	27.432077125328657	23.18141980718668	22.567922874671343	26.818580192813325
32	26.68711656441718	21.779141104294478	22.830850131463627	28.702892199824714
33	26.950043821209462	21.297107800175286	23.707274320771255	28.045574057843996
34	25.942156003505694	22.304995617879054	24.01402278702892	27.73882559158633
35	27.957931638913237	21.910604732690622	24.408413672217353	25.72304995617879
36	27.300613496932513	22.96231375985977	22.611744084136724	27.12532865907099
37	26.468010517090274	21.51621384750219	24.057843996494302	27.957931638913237
38	26.993865030674847	19.193689745836984	25.284837861524977	28.52760736196319
39	25.898334794040316	19.938650306748464	26.292725679228745	27.870289219982467
40	25.197195442594218	21.07800175284838	27.51971954425942	26.205083260297986
41	23.049956178790534	20.858895705521473	27.695004382120946	28.396143733567047
42	21.51621384750219	22.261174408413673	29.009640666082383	27.21297107800175
43	23.751095530236636	21.954425942156004	26.643295354951796	27.651183172655564
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	0.5
4	1.0
5	1.0
6	0.5
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.5
14	1.0
15	4.5
16	8.0
17	7.5
18	7.0
19	7.0
20	4.5
21	2.0
22	4.0
23	6.0
24	5.0
25	4.0
26	4.0
27	6.0
28	8.0
29	9.0
30	10.0
31	17.5
32	25.0
33	25.0
34	22.5
35	20.0
36	28.0
37	36.0
38	50.5
39	65.0
40	65.0
41	66.0
42	67.0
43	75.5
44	84.0
45	110.0
46	136.0
47	136.0
48	169.5
49	203.0
50	211.0
51	219.0
52	244.0
53	269.0
54	269.0
55	249.5
56	230.0
57	210.5
58	191.0
59	173.5
60	156.0
61	156.0
62	154.5
63	153.0
64	137.0
65	121.0
66	104.0
67	87.0
68	87.0
69	65.5
70	44.0
71	44.0
72	44.0
73	35.0
74	26.0
75	26.0
76	27.5
77	29.0
78	19.5
79	10.0
80	8.5
81	7.0
82	7.0
83	6.5
84	6.0
85	4.5
86	3.0
87	2.5
88	2.0
89	2.0
90	1.0
91	0.0
92	0.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2282.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	92.94478527607362
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.67232437529466	87.99298860648554
2	4.054691183404055	7.537248028045573
3	0.7543611504007544	2.1034180543382996
4	0.1885902876001886	0.7011393514461
5	0.1885902876001886	0.8764241893076249
6	0.14144271570014144	0.7887817703768624
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	6	0.26292725679228746	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	6	0.26292725679228746	No Hit
TCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTT	6	0.26292725679228746	TruSeq Adapter, Index 8 (95% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5	0.21910604732690622	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	5	0.21910604732690622	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	5	0.21910604732690622	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	5	0.21910604732690622	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	4	0.175284837861525	No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA	4	0.175284837861525	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	4	0.175284837861525	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	4	0.175284837861525	No Hit
ATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCA	3	0.13146362839614373	No Hit
GGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTC	3	0.13146362839614373	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	3	0.13146362839614373	No Hit
AAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCAAGAC	3	0.13146362839614373	No Hit
TCCTTCATCGCCTCTGACTGCCAGGGCATCCACCGTGTACGCT	3	0.13146362839614373	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	3	0.13146362839614373	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	3	0.13146362839614373	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	3	0.13146362839614373	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	3	0.13146362839614373	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	3	0.13146362839614373	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	3	0.13146362839614373	No Hit
ATCCGGAATGCCCCGACGTTACTCGGACTACCCCGATGCATAC	3	0.13146362839614373	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	3	0.13146362839614373	No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT	3	0.13146362839614373	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	3	0.13146362839614373	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	3	0.13146362839614373	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.04382120946538125	0.0
28	0.0	0.0	0.0	0.0876424189307625	0.0
29	0.0	0.0	0.0	0.21910604732690622	0.0
30	0.0	0.0	0.0	0.3943908851884312	0.0
31	0.0	0.0	0.0	0.9202453987730062	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE