FastQCFastQC Report
Fri 10 Feb 2017
ERR1632724.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632724.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42183
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT1400.3318872531588554TruSeq Adapter, Index 6 (95% over 21bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA740.1754261195268236No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA560.1327549012635422No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA520.1232724083161463No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC500.11853116184244838No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA480.11378991536875045No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA460.10904866889505249No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA450.10667804565820355No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA440.10430742242135457No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG430.10193679918450561No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA200.001821894937.037
AAGGCCG200.001821894937.05
TGAGCAA200.001821894937.036
AATGAGC251.21356476E-437.034
GGTATCA506.9831003E-933.3000031
CCGTCTT352.3345721E-531.71428537
CAATGAG250.00543722329.633
GTCGTCA250.00543722329.620
TCAATGA250.00543722329.632
TCGTCAC250.00543722329.621
TCACCAA250.00543722329.624
GCCGTCT405.8075413E-527.75000236
TACGGGG358.727856E-426.4285715
TATGCCG451.2939137E-424.66666633
CCAATCC400.001900659223.12500227
GCGGTAA400.001900659223.12500223
TTCTGCG400.001900659223.12500218
GCTGTCG400.001900659223.12500217
CGGTGCT400.001900659223.1250029
TAATACG400.001900659223.12500227