FastQCFastQC Report
Fri 10 Feb 2017
ERR1632723.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632723.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147689
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA6350.4299575459242056No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA6170.41776977296887374No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT4580.3101111118634428No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA4250.2877668614453344No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT4160.2816729749676685No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA4140.2803187779726317No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT4090.27693328548503954No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA4060.2749019899924842No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA3800.2572974290570049No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA3630.24578675459919155No Hit
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT3620.24510965610167312Illumina PCR Primer Index 10 (95% over 21bp)
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC3450.23359898164385973No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA3410.230890587653786No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC3350.2268279966686754No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC3210.2173486177034173No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC3190.21599442070838046No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA3110.210577632728233No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG3030.20516084474808552No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA2960.20042115526545645No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC2920.19771276127538273No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC2900.19635856428034587No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC2880.19500436728530898No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA2860.19365017029027212No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA2810.19026467780267994No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC2760.1868791853150878No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA2750.18620208681756933No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC2500.1692746243796085No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG2460.16656623038953478No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT2410.1631807379019426No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTCTGT2410.1631807379019426No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC2410.1631807379019426No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT2280.15437845743420295No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC2260.1530242604391661No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA2240.15167006344412923No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCCTGT2240.15167006344412923No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA2220.15031586644909237No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT2180.14760747245901862No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA2160.14625327546398176No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG2160.14625327546398176No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC2110.14286778297638958No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG2110.14286778297638958No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC2090.14151358598135272No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC2070.14015938898631586No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA2000.1354196995036868No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG1930.13068001002105775No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC1900.12864871452850246No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT1880.1272945175334656No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT1860.12594032053842874No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA1860.12594032053842874No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG1850.12526322204091028No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA1840.12458612354339185No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT1840.12458612354339185No Hit
CACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATC1760.11916933556324438No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT1760.11916933556324438No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT1720.11646094157317065No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC1710.11578384307565223No Hit
TCCTTCATCGCCTCTGACTGCCAGGGCATCCACCGTGTACGCT1710.11578384307565223No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTG1710.11578384307565223No Hit
GTTCATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCC1690.11442964608061534No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTTAT1670.11307544908557847No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT1670.11307544908557847No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA1670.11307544908557847No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC1660.11239835058806005No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC1640.11104415359302318No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC1640.11104415359302318No Hit
TGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTA1580.10698156260791258No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCT1570.10630446411039414No Hit
GATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTGAT1550.10495026711535727No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA1540.10427316861783884No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG1540.10427316861783884No Hit
CATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC1520.10291897162280196No Hit
CTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCT1520.10291897162280196No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC1520.10291897162280196No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG1510.10224187312528354No Hit
CTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAAC1500.1015647746277651No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG1490.10088767613024667No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT1480.10021057763272823No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGAATT251.2284215E-437.020
TATCCTT200.001836745537.05
ATCCTTT200.001836745537.06
AAGTCTG401.5875958E-632.37536
TAAGTCT352.3743403E-531.71428535
CTAAGTC352.3743403E-531.71428534
ACGTTGG303.5844374E-430.83333232
CGTTGGA303.5844374E-430.83333233
TCCAGTG250.005481033629.621
TGGACAG250.005481033629.636
AAGGAAT250.005481033629.619
CAAGGAA250.005481033629.618
CTACTGT250.005481033629.634
ATACGGC652.9449438E-928.46153829
TTAACGT405.905665E-527.75000229
AACACCT405.905665E-527.75000236
AACGTTG358.832684E-426.4285731
TAACGTT358.832684E-426.4285730
TACGGCT706.493792E-926.4285730
GTTGGAC358.832684E-426.4285734
Produced by FastQC (version 0.11.5)