FastQCFastQC Report
Fri 10 Feb 2017
ERR1632722.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632722.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156977
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA4040.2573625435573364No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA2650.168814539709639No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA2560.1630812157195003No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA2540.16180714372169172No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA2530.16117010772278742No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT2490.15862196372717022No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA2490.15862196372717022No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC2390.15225160373812724No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG2340.14906642374360574No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT2250.14333309975346709No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA2160.13759977576332838No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT2080.132503487772094No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA2070.1318664517731897No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA2060.1312294157742854No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT2050.1305923797753811No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA1980.12613312778305102No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT1900.12103683979181663No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC1880.11976276779400803No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA1860.11848869579619944No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC1840.11721462379839084No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA1810.11530351580167796No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC1760.11211833580715645No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA1750.11148129980825215No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT1740.11084426380934788TruSeq Adapter, Index 3 (95% over 21bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC1720.10957019181153926No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC1690.10765908381482638No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC1650.10511093981920919No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA1630.10383686782140059No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT1620.1031998318224963No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC1610.102562795823592No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC1600.1019257598246877No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG1600.1019257598246877No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT1570.10001465182797481No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCAC352.3752877E-531.71428528
GGTATCA2050.031.5853631
TATGGTG303.585458E-430.8333345
ACCCGAG250.005482074329.5999987
ATATGGT358.8351755E-426.428574
AGAACAG358.8351755E-426.428575
ACCAGGT358.8351755E-426.4285733
ACCACCA358.8351755E-426.4285730
GAACAGA358.8351755E-426.428576
GACCTCC451.3161139E-424.66666611
ATACGGC551.8891236E-523.54545429
TGACCTC400.001923806823.12500210
ACTGTAC400.001923806823.1250023
GGTTGCC400.001923806823.12500237
AGAGCAA400.001923806823.12500225
ATACACA603.6999663E-521.58333437
GTATCAA3100.021.4838712
CACTGTG705.0557646E-621.1428577
AACTCCG2050.020.7560965
TCAATAT450.003811327320.5555551
Produced by FastQC (version 0.11.5)