##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632722.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	156977
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.747096708434995	34.0	31.0	34.0	31.0	34.0
2	32.901858234008806	34.0	31.0	34.0	31.0	34.0
3	32.97638507552062	34.0	33.0	34.0	31.0	34.0
4	36.38327907910076	37.0	37.0	37.0	35.0	37.0
5	36.29968084496455	37.0	37.0	37.0	35.0	37.0
6	36.34624817648445	37.0	37.0	37.0	35.0	37.0
7	36.34643928728413	37.0	37.0	37.0	35.0	37.0
8	36.31847340693223	37.0	37.0	37.0	35.0	37.0
9	38.05442198538639	39.0	38.0	39.0	37.0	39.0
10	38.055199169305055	39.0	38.0	39.0	37.0	39.0
11	38.13136319333405	39.0	38.0	39.0	37.0	39.0
12	38.08498697261382	39.0	38.0	39.0	37.0	39.0
13	38.13574600100652	39.0	38.0	39.0	37.0	39.0
14	39.54869821693624	40.0	39.0	41.0	37.0	41.0
15	39.53433305516095	40.0	39.0	41.0	37.0	41.0
16	39.478815367856434	40.0	39.0	41.0	37.0	41.0
17	39.48868305547946	40.0	39.0	41.0	37.0	41.0
18	39.494021417150286	40.0	39.0	41.0	37.0	41.0
19	39.49316778891175	40.0	39.0	41.0	37.0	41.0
20	39.469648419832204	40.0	39.0	41.0	37.0	41.0
21	39.434127292533304	40.0	39.0	41.0	37.0	41.0
22	39.39679061263752	40.0	39.0	41.0	37.0	41.0
23	39.35256120323359	40.0	39.0	41.0	36.0	41.0
24	39.32605413531919	40.0	39.0	41.0	36.0	41.0
25	39.28285672423349	40.0	39.0	41.0	36.0	41.0
26	39.17594934289737	40.0	39.0	41.0	36.0	41.0
27	39.073692324353246	40.0	39.0	41.0	35.0	41.0
28	39.04154748784854	40.0	39.0	41.0	35.0	41.0
29	38.995311415048064	40.0	39.0	41.0	35.0	41.0
30	38.94281327837836	40.0	38.0	41.0	35.0	41.0
31	38.872981392178474	40.0	38.0	41.0	35.0	41.0
32	38.82306962166432	40.0	38.0	41.0	35.0	41.0
33	38.76009224281264	40.0	38.0	41.0	35.0	41.0
34	38.718602088204	40.0	38.0	41.0	35.0	41.0
35	38.621600616650845	40.0	38.0	41.0	35.0	41.0
36	38.56696840938481	40.0	38.0	41.0	35.0	41.0
37	38.50957783624352	40.0	38.0	41.0	35.0	41.0
38	38.450403562305304	40.0	38.0	41.0	35.0	41.0
39	38.351478242035455	40.0	38.0	41.0	34.0	41.0
40	38.2743013307682	40.0	38.0	41.0	34.0	41.0
41	38.229747033004834	40.0	37.0	41.0	34.0	41.0
42	38.16741306051205	40.0	37.0	41.0	34.0	41.0
43	37.401339049669694	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	9.0
19	3.0
20	13.0
21	35.0
22	39.0
23	61.0
24	127.0
25	180.0
26	262.0
27	352.0
28	548.0
29	696.0
30	941.0
31	1275.0
32	1672.0
33	2274.0
34	3616.0
35	5419.0
36	8386.0
37	16664.0
38	43093.0
39	71311.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.63936755066029	16.311943787943456	13.265000605184198	27.783688056212057
2	21.439446543124152	19.63536059422718	32.35442134835039	26.570771514298276
3	21.352172611274263	19.534071870401394	29.498588965262428	29.61516655306191
4	16.095351548315996	16.13548481624697	33.82661154181823	33.94255209361881
5	17.375793906113636	32.66147269982227	32.63535422386719	17.327379170196906
6	33.95975206558923	33.528478694331014	15.453219261420461	17.058549978659293
7	29.76996630079566	27.890710104027978	19.671671646164725	22.66765194901164
8	27.181689037247498	30.61786121533728	20.603655312561713	21.596794434853514
9	28.44811660306924	12.784675462010359	17.310816234225396	41.45639170069501
10	19.80608624193353	23.1212215802315	29.099167393949433	27.97352478388554
11	39.615994699860494	19.32830924275531	18.44410327627614	22.61159278110806
12	23.143517840193148	23.614924479382328	26.326149690719024	26.915407989705496
13	35.77530466246648	17.091675850602318	20.69793664039955	26.43508284653166
14	24.257693802276766	20.316352077055875	23.146065984188766	32.27988813647859
15	30.200602636054963	24.10544219853864	19.71053084209789	25.983424323308512
16	25.24509960057843	23.975486854762163	22.387356109493748	28.39205743516566
17	27.177229785255165	23.467769163635438	21.70445351866834	27.65054753244106
18	27.122444689349397	20.64888486848392	23.20276218809125	29.02590825407544
19	28.811227122444688	21.86753473438784	22.940940392541584	26.380297750625886
20	30.426113379667086	20.384514928938636	22.466985609356787	26.722386082037495
21	27.921287831975384	21.772616370551102	21.57131299489734	28.734782802576177
22	28.543672002904884	22.68994820897329	20.954025111959076	27.812354676162748
23	28.642412582735048	21.598705542850226	21.87900138236812	27.879880492046606
24	28.15635411557107	21.40504659918332	22.416659765443345	28.021939519802263
25	28.32262051128509	21.469387235072652	22.995725488447352	27.212266765194904
26	28.398427795154703	22.316008077616466	22.206437885804927	27.0791262414239
27	26.889926549749326	21.85734215840537	24.3366862661409	26.916045025704406
28	26.67142320212515	22.845384992705938	21.85415697841085	28.62903482675806
29	27.325659172999867	23.429547003701177	22.124897277945177	27.11989654535378
30	26.893111729743847	22.139549105919976	23.717487275205922	27.249851889130255
31	28.32262051128509	22.19051198583232	22.09750472999229	27.389362772890298
32	25.490358460156582	21.8911050663473	22.777859176822083	29.840677296674034
33	26.135675927046638	21.249609815450672	24.8259299132994	27.788784344203293
34	26.25034240684941	21.35918000726221	24.18252355440606	28.20795403148232
35	26.152875899017054	23.014199532415578	24.84822617326105	25.98469839530632
36	25.94074291138192	21.54455748294336	25.173114532702247	27.341585072972475
37	26.335705230702587	21.146409983628175	25.0775591328666	27.44032565280264
38	25.61840269593635	20.217611497225707	26.39431254260178	27.76967326423616
39	24.724641189473616	19.089420743166198	27.97416181988444	28.211776247475743
40	24.30547150219459	20.229078145205985	29.072411881995453	26.393038470603976
41	22.875962720653344	19.28690190282653	30.003758512393535	27.833376864126592
42	20.967402867936066	20.236722577192836	31.299489734164876	27.496384820706215
43	19.933493441714393	20.90561037604235	30.731253623142248	28.429642559101016
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3.0
1	2.5
2	2.0
3	4.5
4	7.0
5	7.0
6	11.5
7	16.0
8	17.5
9	19.0
10	21.0
11	23.0
12	23.0
13	48.0
14	73.0
15	149.5
16	226.0
17	215.5
18	205.0
19	205.0
20	251.0
21	297.0
22	257.5
23	218.0
24	278.5
25	339.0
26	339.0
27	407.0
28	475.0
29	659.5
30	844.0
31	977.5
32	1111.0
33	1111.0
34	1378.0
35	1645.0
36	1909.5
37	2174.0
38	2736.0
39	3298.0
40	3298.0
41	3985.5
42	4673.0
43	5598.5
44	6524.0
45	8846.0
46	11168.0
47	11168.0
48	12845.0
49	14522.0
50	16114.0
51	17706.0
52	18255.5
53	18805.0
54	18805.0
55	17285.5
56	15766.0
57	15166.5
58	14567.0
59	13486.5
60	12406.0
61	12406.0
62	11154.5
63	9903.0
64	8018.5
65	6134.0
66	5278.0
67	4422.0
68	4422.0
69	3738.5
70	3055.0
71	2603.0
72	2151.0
73	1756.0
74	1361.0
75	1361.0
76	1207.0
77	1053.0
78	859.0
79	665.0
80	553.5
81	442.0
82	442.0
83	378.5
84	315.0
85	242.5
86	170.0
87	138.5
88	107.0
89	107.0
90	80.5
91	54.0
92	36.5
93	19.0
94	15.5
95	12.0
96	12.0
97	7.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	156977.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.55590946444383
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.14275885792914	47.30183402664084
2	6.667813324160074	7.4087286672569865
3	2.4974200206398347	4.162393216840684
4	1.3656690746474027	3.034839498780076
5	0.8565531475748194	2.3793294559075533
6	0.5939685815846807	1.9799078845945586
7	0.4517830524022474	1.756945284978054
8	0.32565072812750834	1.4473457895105653
9	0.27290448343079926	1.3645311096530066
>10	1.6030271757825936	16.65785433534849
>50	0.14906547414287352	5.80403498601706
>100	0.07338607957803003	6.7022557444721205
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	404	0.2573625435573364	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	265	0.168814539709639	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	256	0.1630812157195003	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	254	0.16180714372169172	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	253	0.16117010772278742	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	249	0.15862196372717022	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	249	0.15862196372717022	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	239	0.15225160373812724	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	234	0.14906642374360574	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	225	0.14333309975346709	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	216	0.13759977576332838	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	208	0.132503487772094	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	207	0.1318664517731897	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	206	0.1312294157742854	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	205	0.1305923797753811	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	198	0.12613312778305102	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	190	0.12103683979181663	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	188	0.11976276779400803	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	186	0.11848869579619944	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	184	0.11721462379839084	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	181	0.11530351580167796	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	176	0.11211833580715645	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	175	0.11148129980825215	No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT	174	0.11084426380934788	TruSeq Adapter, Index 3 (95% over 21bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	172	0.10957019181153926	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	169	0.10765908381482638	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	165	0.10511093981920919	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	163	0.10383686782140059	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	162	0.1031998318224963	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	161	0.102562795823592	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	160	0.1019257598246877	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	160	0.1019257598246877	No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT	157	0.10001465182797481	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0019111079967128943	0.0
17	6.370359989042981E-4	0.0	0.0	0.0025481439956171925	0.0
18	6.370359989042981E-4	0.0	0.0	0.0038222159934257886	0.0
19	6.370359989042981E-4	0.0	0.0	0.0038222159934257886	0.0
20	6.370359989042981E-4	0.0	0.0	0.005733323990138683	0.0
21	6.370359989042981E-4	0.0	0.0	0.007644431986851577	0.0
22	6.370359989042981E-4	0.0	0.0	0.01019257598246877	0.0
23	6.370359989042981E-4	0.0	0.0	0.012740719978085962	0.0
24	6.370359989042981E-4	0.0	0.0	0.017199971970416048	0.0
25	6.370359989042981E-4	0.0	0.0	0.018474043968224645	0.0
26	6.370359989042981E-4	0.0	0.0	0.02229625996165043	0.0
27	6.370359989042981E-4	0.0	0.0	0.05478509590576963	0.0
28	6.370359989042981E-4	0.0	0.0	0.1936589436669066	0.0
29	6.370359989042981E-4	0.0	0.0	0.39050306732833473	0.0
30	6.370359989042981E-4	0.0	0.0	0.6918210948100677	0.0
31	6.370359989042981E-4	0.0	0.0	1.4817457334513973	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGACCAC	35	2.3752877E-5	31.714285	28
GGTATCA	205	0.0	31.585363	1
TATGGTG	30	3.585458E-4	30.833334	5
ACCCGAG	25	0.0054820743	29.599998	7
ATATGGT	35	8.8351755E-4	26.42857	4
AGAACAG	35	8.8351755E-4	26.42857	5
ACCAGGT	35	8.8351755E-4	26.42857	33
ACCACCA	35	8.8351755E-4	26.42857	30
GAACAGA	35	8.8351755E-4	26.42857	6
GACCTCC	45	1.3161139E-4	24.666666	11
ATACGGC	55	1.8891236E-5	23.545454	29
TGACCTC	40	0.0019238068	23.125002	10
ACTGTAC	40	0.0019238068	23.125002	3
GGTTGCC	40	0.0019238068	23.125002	37
AGAGCAA	40	0.0019238068	23.125002	25
ATACACA	60	3.6999663E-5	21.583334	37
GTATCAA	310	0.0	21.483871	2
CACTGTG	70	5.0557646E-6	21.142857	7
AACTCCG	205	0.0	20.756096	5
TCAATAT	45	0.0038113273	20.555555	1
>>END_MODULE