FastQCFastQC Report
Fri 10 Feb 2017
ERR1632721.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632721.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86986
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA1780.20463063021635666No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA1590.18278803485618375No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCGGGCTGCGTCT1540.17703998344561195No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA1370.15749660864966775No Hit
TCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT1330.1528981675212103RNA PCR Primer, Index 41 (95% over 22bp)
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA1280.1471501161106385No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGGTCTT1160.13335479272526615No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1090.1253075207504656No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA1040.11955946933989378No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC1030.11840985905777941No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA960.11036258708297887No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT920.10576414595452141No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT880.10116570482606396No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT880.10116570482606396No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT880.10116570482606396No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT880.10116570482606396No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG880.10116570482606396No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGTC251.2242646E-437.04
GGTCTTC200.001832595437.07
GGTATCA850.032.647061
TCGTCTT250.00546879129.614
AGGGTCT358.803355E-426.428575
GTATCAA1150.024.1304362
CCATAGG400.001916943623.1250021
CCAGGAC502.6743714E-422.23
TCCAGGA603.675559E-521.5833342
TTCTGCG450.0037978620.55555718
TCTGCGG500.00698398418.519
AACGTCA500.00698398418.528
CGGGTAA500.00698398418.523
TGCGGGT500.00698398418.521
CTTATAC1103.731293E-818.537
AATGAGC500.00698398418.534
CAATGAG500.00698398418.533
GTAACGT500.00698398418.526
CTCTTAT2950.015.67796637
CCCCAAT1556.9048838E-915.5161291