##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632721.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	86986
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.014105718161545	34.0	33.0	34.0	31.0	34.0
2	33.15382935184972	34.0	33.0	34.0	31.0	34.0
3	33.23040489274136	34.0	34.0	34.0	31.0	34.0
4	36.5324075138528	37.0	37.0	37.0	35.0	37.0
5	36.49348170970041	37.0	37.0	37.0	35.0	37.0
6	36.536959970569974	37.0	37.0	37.0	35.0	37.0
7	36.53522405904398	37.0	37.0	37.0	35.0	37.0
8	36.50648380199112	37.0	37.0	37.0	35.0	37.0
9	38.30618720253834	39.0	39.0	39.0	37.0	39.0
10	38.339146529326555	39.0	39.0	39.0	37.0	39.0
11	38.41725105189341	39.0	39.0	39.0	37.0	39.0
12	38.382130457774814	39.0	39.0	39.0	37.0	39.0
13	38.40090359368174	39.0	39.0	39.0	37.0	39.0
14	39.94128940289242	41.0	40.0	41.0	38.0	41.0
15	39.946566114087325	41.0	40.0	41.0	38.0	41.0
16	39.91110063688409	41.0	40.0	41.0	38.0	41.0
17	39.90912330719886	41.0	40.0	41.0	38.0	41.0
18	39.86894442783896	41.0	40.0	41.0	38.0	41.0
19	39.88559078472398	41.0	40.0	41.0	38.0	41.0
20	39.859862506610256	41.0	40.0	41.0	38.0	41.0
21	39.82942082633987	41.0	40.0	41.0	38.0	41.0
22	39.80426735336721	41.0	40.0	41.0	38.0	41.0
23	39.75896121214908	41.0	40.0	41.0	38.0	41.0
24	39.74163658519762	41.0	40.0	41.0	38.0	41.0
25	39.67829305865312	41.0	40.0	41.0	37.0	41.0
26	39.58339272986458	41.0	40.0	41.0	37.0	41.0
27	39.50963373416412	41.0	40.0	41.0	37.0	41.0
28	39.46830524452211	41.0	39.0	41.0	37.0	41.0
29	39.4137677327386	41.0	39.0	41.0	36.0	41.0
30	39.37946336192031	41.0	39.0	41.0	36.0	41.0
31	39.33293863380314	41.0	39.0	41.0	36.0	41.0
32	39.2679971489665	41.0	39.0	41.0	36.0	41.0
33	39.20423976272044	41.0	39.0	41.0	35.0	41.0
34	39.14260915549629	41.0	39.0	41.0	35.0	41.0
35	39.05988319959534	41.0	39.0	41.0	35.0	41.0
36	39.02333708872692	41.0	39.0	41.0	35.0	41.0
37	38.9846641988366	40.0	39.0	41.0	35.0	41.0
38	38.88844181822362	40.0	39.0	41.0	35.0	41.0
39	38.812383601958935	40.0	38.0	41.0	35.0	41.0
40	38.71303428137861	40.0	38.0	41.0	35.0	41.0
41	38.668440898535394	40.0	38.0	41.0	35.0	41.0
42	38.607810452256686	40.0	38.0	41.0	35.0	41.0
43	37.862023773940635	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	6.0
20	1.0
21	6.0
22	21.0
23	33.0
24	49.0
25	69.0
26	132.0
27	143.0
28	210.0
29	268.0
30	393.0
31	484.0
32	711.0
33	903.0
34	1449.0
35	2478.0
36	3561.0
37	6571.0
38	17141.0
39	52356.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.69208838203849	15.681833858322028	11.891568758190974	28.73450900144851
2	21.815004713402157	17.948865334651554	31.68555859563608	28.55057135631021
3	21.335617225760465	19.13181431494723	28.32869657186214	31.20387188743016
4	16.207205757248293	14.683972133446762	32.38452164716161	36.72430046214333
5	17.871841445749894	32.24082036189731	32.41671073506082	17.47062745729198
6	37.08297887016301	32.24541880302577	14.39656956291817	16.27503276389304
7	32.122410502839536	26.461729473708413	18.866254339778816	22.549605683673235
8	28.912698595176234	29.78295357873681	19.49279194353114	21.811555882555815
9	28.5494217460281	12.414641436553008	16.61531740739889	42.42061941002
10	20.530890028280414	22.2668015542731	28.888556779251832	28.313751638194653
11	40.33062791713609	18.59609592348194	17.936219621548293	23.137056537833676
12	24.27861954797324	22.394408295587795	25.56273423309498	27.764237923343988
13	35.03437334743522	16.802703883383533	20.137723311797302	28.02519945738395
14	24.972984158370313	18.974317706297565	21.856390683558274	34.19630745177385
15	29.982985767824704	22.692157358655415	19.464051686478285	27.860805187041592
16	27.146897201848574	22.63582645483181	20.713678063136598	29.50359828018302
17	27.645828064286203	22.92552824592463	20.95509622238061	28.47354746740855
18	27.064125261536336	20.36419653737383	22.839307474766056	29.732370726323776
19	29.20010116570483	21.194215161060402	21.767870691835466	27.837812981399306
20	29.40358218563907	20.146920194054214	22.141494033522633	28.308003586784082
21	28.908100154047776	20.773457797806543	21.33906605660681	28.97937599153887
22	30.13588393534592	21.77591796381027	20.054951371485068	28.033246729358748
23	29.51969282413262	21.5942795392362	20.7458671510358	28.140160485595384
24	29.441519324948846	21.04706504494976	20.826339870783805	28.68507575931759
25	28.81958016232497	21.50231071666705	21.84029613960867	27.837812981399306
26	29.561078794288736	21.37355436507024	21.47931851102476	27.586048329616258
27	28.51033499643621	21.145931529211598	22.60593658749684	27.737796886855353
28	27.39291380222105	21.56324006161911	22.07711585772423	28.96673027843561
29	28.750603545398114	22.11390338675189	21.50460993723128	27.63088313061872
30	27.303244200216128	20.876922723196838	23.410663784976894	28.40916929161014
31	28.02864828823029	21.21260892557423	21.848343411583475	28.910399374612005
32	26.705446853516655	21.196514381624628	22.37486492079185	29.723173844066864
33	27.330834846986875	21.181569447957145	23.39456924102729	28.093026464028693
34	27.668820269928496	20.994182971972503	23.185340169682476	28.15165658841653
35	27.64467845400409	22.28404570850482	23.41986066723381	26.65141517025728
36	27.199779274825836	21.076954912284734	23.346285609178487	28.376980203710943
37	26.580139332766194	20.897615708274895	23.991216977444648	28.531027981514267
38	26.507713884992988	20.073345135998895	25.589175269583613	27.829765709424503
39	25.192559722254153	19.522681810866118	26.422642724116525	28.862115742763205
40	25.184512450279357	20.12392798841193	27.89414388522291	26.797415676085805
41	23.740601935943715	19.237578460901755	28.145908537005955	28.875911066148575
42	21.988595866001425	20.103235003333868	29.66684294024326	28.241326190421447
43	21.87593405835422	19.746855815878416	29.33000712758375	29.047202998183614
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	1.0
4	1.0
5	1.0
6	2.5
7	4.0
8	3.0
9	2.0
10	5.0
11	8.0
12	8.0
13	19.0
14	30.0
15	47.5
16	65.0
17	66.0
18	67.0
19	67.0
20	77.5
21	88.0
22	69.5
23	51.0
24	58.5
25	66.0
26	66.0
27	89.0
28	112.0
29	170.0
30	228.0
31	310.0
32	392.0
33	392.0
34	580.5
35	769.0
36	900.0
37	1031.0
38	1418.5
39	1806.0
40	1806.0
41	2134.5
42	2463.0
43	2998.5
44	3534.0
45	4396.5
46	5259.0
47	5259.0
48	5832.5
49	6406.0
50	7418.0
51	8430.0
52	9033.5
53	9637.0
54	9637.0
55	9293.0
56	8949.0
57	8591.0
58	8233.0
59	7706.5
60	7180.0
61	7180.0
62	6922.0
63	6664.0
64	5493.5
65	4323.0
66	3774.0
67	3225.0
68	3225.0
69	2737.0
70	2249.0
71	1973.5
72	1698.0
73	1681.0
74	1664.0
75	1664.0
76	1586.0
77	1508.0
78	999.0
79	490.0
80	330.5
81	171.0
82	171.0
83	134.0
84	97.0
85	70.5
86	44.0
87	36.0
88	28.0
89	28.0
90	19.0
91	10.0
92	6.0
93	2.0
94	1.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	86986.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.36242613753937
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.21215407556598	50.5840020233141
2	6.578616108604296	7.810452256684984
3	2.6357069543157037	4.693858781872945
4	1.3943490133044136	3.3108776124893664
5	0.8753413250188817	2.598119237578461
6	0.5790421596916939	2.0624008461131678
7	0.3834459786587137	1.5933598510105074
8	0.2536940565873308	1.2047915756558527
9	0.25563065243914246	1.3657370151518635
>10	1.7158239247051534	19.430712988296968
>50	0.09682979259058427	3.827052629158715
>100	0.019365958518116854	1.5186351826730737
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	178	0.20463063021635666	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	159	0.18278803485618375	No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCGGGCTGCGTCT	154	0.17703998344561195	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	137	0.15749660864966775	No Hit
TCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT	133	0.1528981675212103	RNA PCR Primer, Index 41 (95% over 22bp)
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	128	0.1471501161106385	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGGTCTT	116	0.13335479272526615	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	109	0.1253075207504656	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	104	0.11955946933989378	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	103	0.11840985905777941	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	96	0.11036258708297887	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	92	0.10576414595452141	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	88	0.10116570482606396	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	88	0.10116570482606396	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	88	0.10116570482606396	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	88	0.10116570482606396	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	88	0.10116570482606396	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0011496102821143632	0.0	0.0	0.0	0.0
6	0.0011496102821143632	0.0	0.0	0.0	0.0
7	0.0011496102821143632	0.0	0.0	0.0	0.0
8	0.0011496102821143632	0.0	0.0	0.0	0.0
9	0.0011496102821143632	0.0	0.0	0.0	0.0
10	0.0011496102821143632	0.0	0.0	0.0	0.0
11	0.0011496102821143632	0.0	0.0	0.0	0.0
12	0.0011496102821143632	0.0	0.0	0.0	0.0
13	0.0011496102821143632	0.0	0.0	0.0	0.0
14	0.0011496102821143632	0.0	0.0	0.0	0.0
15	0.0022992205642287265	0.0	0.0	0.0	0.0
16	0.0022992205642287265	0.0	0.0	0.0	0.0
17	0.0022992205642287265	0.0	0.0	0.0	0.0
18	0.0022992205642287265	0.0	0.0	0.0	0.0
19	0.0022992205642287265	0.0	0.0	0.0	0.0
20	0.0022992205642287265	0.0	0.0	0.0022992205642287265	0.0
21	0.0022992205642287265	0.0	0.0	0.0022992205642287265	0.0
22	0.0022992205642287265	0.0	0.0	0.004598441128457453	0.0
23	0.0022992205642287265	0.0	0.0	0.004598441128457453	0.0
24	0.0022992205642287265	0.0	0.0	0.0068976616926861795	0.0
25	0.0022992205642287265	0.0	0.0	0.009196882256914906	0.0
26	0.0022992205642287265	0.0	0.0	0.013795323385372359	0.0
27	0.0022992205642287265	0.0	0.0	0.03908674959188835	0.0
28	0.0022992205642287265	0.0	0.0	0.14140206470006667	0.0
29	0.0022992205642287265	0.0	0.0	0.332237371531051	0.0
30	0.0022992205642287265	0.0	0.0	0.5403168325937507	0.0
31	0.0022992205642287265	0.0	0.0	1.1335157381647623	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGGTC	25	1.2242646E-4	37.0	4
GGTCTTC	20	0.0018325954	37.0	7
GGTATCA	85	0.0	32.64706	1
TCGTCTT	25	0.005468791	29.6	14
AGGGTCT	35	8.803355E-4	26.42857	5
GTATCAA	115	0.0	24.130436	2
CCATAGG	40	0.0019169436	23.125002	1
CCAGGAC	50	2.6743714E-4	22.2	3
TCCAGGA	60	3.675559E-5	21.583334	2
TTCTGCG	45	0.00379786	20.555557	18
TCTGCGG	50	0.006983984	18.5	19
AACGTCA	50	0.006983984	18.5	28
CGGGTAA	50	0.006983984	18.5	23
TGCGGGT	50	0.006983984	18.5	21
CTTATAC	110	3.731293E-8	18.5	37
AATGAGC	50	0.006983984	18.5	34
CAATGAG	50	0.006983984	18.5	33
GTAACGT	50	0.006983984	18.5	26
CTCTTAT	295	0.0	15.677966	37
CCCCAAT	155	6.9048838E-9	15.516129	1
>>END_MODULE