FastQCFastQC Report
Fri 10 Feb 2017
ERR1632720.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632720.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1412822
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA32930.23307960946248008No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA31510.22302880334536127No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA28330.2005206600689967No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA27700.19606149960858482No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA26330.18636459511530823No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG25730.1821177756292017No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC25320.17921578231369556No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA24950.1765969102972632No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT23880.1690234155470399No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC22340.1581232455326998No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT21690.15352252442275108No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA21690.15352252442275108No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA20110.1423392331093372No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT19780.1400034823919786No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA19660.13915411849475728No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT19240.13618134485448272No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT18330.12974033530055448No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA18230.12903253205287007No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA16640.11777846041468776No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC16550.11714143749177179No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT16400.11607973262024517No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA15670.11091276891214888No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC15490.10963872306631692No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG15440.1092848214424747No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA15320.1084354575452534No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA14870.10525034293067351No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC14850.10510878228113661No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA14730.10425941838391531No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC14570.10312693318762023No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA14520.10277303156377804No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG14160.10022493987211412No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24750.030.496971
CCCGTTA358.8704063E-426.428578
GCCTAGT1002.0008883E-1124.0500011
ACACGAA1950.020.87179617
ATACGGC2450.020.38775429
GTATCAA36950.020.3775372
TTTTACG1001.2903911E-820.354
TAGCGAA1950.019.92307710
AAACACG2150.019.79069715
CTTATAC20950.019.25059737
GCGGTAA13150.019.1330823
TGCGGGT9050.019.0110521
GGACGTA2050.018.95122127
GCAGTCG3950.018.7341779
CTAGATA609.238246E-418.53
GGTCGTG1002.876077E-718.511
TAGAACG500.007035660618.54
CGTTAGA1601.8189894E-1218.51
TCGTGTA801.6170983E-518.513
GTCGTGT902.1529268E-618.512
Produced by FastQC (version 0.11.5)