##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632720.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1412822
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.00796844896243	34.0	33.0	34.0	31.0	34.0
2	33.142703751781895	34.0	33.0	34.0	31.0	34.0
3	33.21478855793582	34.0	33.0	34.0	31.0	34.0
4	36.51002886421644	37.0	37.0	37.0	35.0	37.0
5	36.48573280993642	37.0	37.0	37.0	35.0	37.0
6	36.53494141512519	37.0	37.0	37.0	35.0	37.0
7	36.53856890676957	37.0	37.0	37.0	35.0	37.0
8	36.516015464085356	37.0	37.0	37.0	35.0	37.0
9	38.30286334725818	39.0	39.0	39.0	37.0	39.0
10	38.33606073518108	39.0	39.0	39.0	37.0	39.0
11	38.40175761702465	39.0	39.0	39.0	37.0	39.0
12	38.35987619105592	39.0	39.0	39.0	37.0	39.0
13	38.39369219901729	39.0	39.0	39.0	37.0	39.0
14	39.91250419373424	41.0	40.0	41.0	38.0	41.0
15	39.91174259743973	41.0	40.0	41.0	38.0	41.0
16	39.86260406477249	41.0	40.0	41.0	38.0	41.0
17	39.87836825870492	41.0	40.0	41.0	38.0	41.0
18	39.89191773627534	41.0	40.0	41.0	38.0	41.0
19	39.895616008244495	41.0	40.0	41.0	38.0	41.0
20	39.877917388036145	41.0	40.0	41.0	38.0	41.0
21	39.83872561440861	41.0	40.0	41.0	38.0	41.0
22	39.80589982319075	41.0	40.0	41.0	38.0	41.0
23	39.771585521743006	41.0	40.0	41.0	38.0	41.0
24	39.745941102276156	41.0	40.0	41.0	38.0	41.0
25	39.70877293813375	41.0	40.0	41.0	38.0	41.0
26	39.62401916164952	41.0	40.0	41.0	37.0	41.0
27	39.533374338734816	41.0	40.0	41.0	37.0	41.0
28	39.500830253209536	41.0	40.0	41.0	37.0	41.0
29	39.46600491781697	41.0	39.0	41.0	37.0	41.0
30	39.41373577138521	41.0	39.0	41.0	36.0	41.0
31	39.352270845159545	41.0	39.0	41.0	36.0	41.0
32	39.303585306570824	41.0	39.0	41.0	36.0	41.0
33	39.23909381365805	41.0	39.0	41.0	35.0	41.0
34	39.203496264922265	41.0	39.0	41.0	35.0	41.0
35	39.1237664758901	41.0	39.0	41.0	35.0	41.0
36	39.07232404365164	41.0	39.0	41.0	35.0	41.0
37	39.01219049533487	41.0	39.0	41.0	35.0	41.0
38	38.94283073168453	41.0	39.0	41.0	35.0	41.0
39	38.85471772098679	40.0	39.0	41.0	35.0	41.0
40	38.776097767447	40.0	38.0	41.0	35.0	41.0
41	38.71503204225302	40.0	38.0	41.0	35.0	41.0
42	38.63535392285794	40.0	38.0	41.0	35.0	41.0
43	37.919878795771865	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	0.0
15	1.0
16	6.0
17	2.0
18	12.0
19	37.0
20	66.0
21	132.0
22	278.0
23	467.0
24	794.0
25	1190.0
26	1784.0
27	2590.0
28	3393.0
29	4665.0
30	6557.0
31	8397.0
32	11151.0
33	14986.0
34	22665.0
35	35836.0
36	55663.0
37	107945.0
38	276386.0
39	857818.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.91481871035417	16.324137081670585	12.997744938852879	27.763299269122367
2	21.088926984432575	19.228112246270232	32.78962247190375	26.89333829739344
3	21.38776151560494	19.7157886839248	29.276441052022122	29.62000874844814
4	15.547323017336934	16.353298575475183	34.082000421850736	34.01737798533715
5	17.523226563572763	32.17050697115419	32.89883651302146	17.407429952251594
6	33.033389910406264	33.69688467478564	15.98863834226817	17.281087072539925
7	29.730850737035517	27.822259279654478	20.103098621057715	22.343791362252286
8	26.839049788296048	30.677466800488666	20.520773317516287	21.962710093698995
9	28.293302340988465	13.051537985676894	18.149278536149634	40.505881137185014
10	19.89019140415424	23.23958715252169	29.159724296478963	27.71049714684511
11	38.840420095383564	19.718832237889842	18.801590009215598	22.639157657510996
12	22.74426643979213	24.04018340597754	26.619843122488184	26.595707031742144
13	35.24449647584763	17.871678102407806	20.817413658620833	26.06641176312373
14	23.908744342882542	20.80580568535881	23.629091279722427	31.656358692036225
15	29.8748886979393	24.017816823350714	20.411842397697658	25.695452081012327
16	24.93994289443398	24.113157920813805	23.138512848752356	27.808386335999863
17	26.530943034579018	23.831947690508784	22.095706323938895	27.5414029509733
18	26.740594356543145	21.327102777278384	23.55293165027158	28.379371215906886
19	28.058382443082003	22.534615117828007	23.56269933508963	25.84430310400036
20	29.793986786728972	20.81104342939167	23.12336585925191	26.271603924627446
21	27.379032885954498	22.37266973475781	22.333811336459934	27.914486042827757
22	28.152378714374493	23.33082299114821	21.622893754485702	26.893904539991592
23	28.11196314893171	22.026483166315362	22.785035906858756	27.07651777789417
24	27.64268959571694	21.824900801374834	23.119189820090572	27.41321978281765
25	27.61338654126281	22.217590043190157	23.489229357979983	26.67979405756705
26	27.513586283339304	23.032342361599692	22.75976733091642	26.694304024144582
27	26.75814787708572	22.139448564645793	24.703748950681685	26.398654607586803
28	26.138536914062776	23.093992024473007	22.548841963106465	28.218629098357756
29	27.037942500895372	23.620526860425446	22.709796421629903	26.63173421704928
30	26.595140789143993	22.183827828275607	24.276660471028904	26.94437091155149
31	27.573112536469562	22.297005567580346	23.10368892896628	27.026192966983807
32	25.247412625228087	22.339827664065254	23.29953808760056	29.113221623106096
33	25.67046662636907	21.66649443454306	25.243802828664897	27.41923611042297
34	25.80749733512077	21.53710800086635	24.950489162824475	27.704905501188403
35	25.45402039322717	23.242206024538124	25.14520583626246	26.158567745972245
36	25.794827656987223	21.78802425217048	25.581283417160833	26.83586467368147
37	26.007310191942086	21.501363936858287	25.362218312002504	27.129107559197124
38	25.12552890597683	20.540096346178075	26.509779717473254	27.824595030371835
39	24.671543902912045	19.58661459122239	27.92312124244951	27.818720263416058
40	24.27637734972983	20.746279432228548	28.544997175865046	26.43234604217658
41	22.729968814188908	19.98503703934395	29.52318126416491	27.761812882302227
42	21.065711037908528	20.893077825798294	30.44332548615466	27.597885650138515
43	20.457495707173305	21.54906987575222	29.817273513577792	28.17616090349669
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	16.0
1	18.5
2	21.0
3	70.5
4	120.0
5	120.0
6	157.0
7	194.0
8	191.5
9	189.0
10	313.0
11	437.0
12	437.0
13	863.0
14	1289.0
15	2300.0
16	3311.0
17	3126.0
18	2941.0
19	2941.0
20	3520.5
21	4100.0
22	3390.0
23	2680.0
24	2973.0
25	3266.0
26	3266.0
27	3848.0
28	4430.0
29	5429.0
30	6428.0
31	7955.0
32	9482.0
33	9482.0
34	12993.0
35	16504.0
36	18316.5
37	20129.0
38	25917.0
39	31705.0
40	31705.0
41	38331.5
42	44958.0
43	54352.0
44	63746.0
45	87258.5
46	110771.0
47	110771.0
48	125061.5
49	139352.0
50	153630.5
51	167909.0
52	167656.5
53	167404.0
54	167404.0
55	152898.0
56	138392.0
57	130937.5
58	123483.0
59	112959.5
60	102436.0
61	102436.0
62	92002.0
63	81568.0
64	67039.5
65	52511.0
66	45658.5
67	38806.0
68	38806.0
69	33047.5
70	27289.0
71	22638.0
72	17987.0
73	14318.5
74	10650.0
75	10650.0
76	8671.0
77	6692.0
78	5518.5
79	4345.0
80	3681.0
81	3017.0
82	3017.0
83	2507.5
84	1998.0
85	1537.5
86	1077.0
87	884.5
88	692.0
89	692.0
90	500.0
91	308.0
92	214.0
93	120.0
94	88.5
95	57.0
96	57.0
97	33.0
98	9.0
99	6.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1412822.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.1177224153696
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.5804677277364	35.62336661518929
2	8.07605793926288	6.802903329926317
3	2.2666552207564545	2.86399066197506
4	1.1051042266768494	1.861778922369089
5	0.6857164023671042	1.4440406545281792
6	0.4657533206005548	1.1769881442654486
7	0.35285518395302756	1.0403019683390455
8	0.2527736068100388	0.8516998884445596
9	0.21643656241482453	0.820423355069196
>10	1.569082306953799	13.194139498629129
>50	0.21668427662927237	6.395133769211005
>100	0.18235793879122242	15.201133222305177
>500	0.0194177416318694	5.633646980530737
>1k	0.010637545415719757	7.0904529892178045
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	3293	0.23307960946248008	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	3151	0.22302880334536127	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	2833	0.2005206600689967	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	2770	0.19606149960858482	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	2633	0.18636459511530823	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	2573	0.1821177756292017	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	2532	0.17921578231369556	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	2495	0.1765969102972632	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	2388	0.1690234155470399	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	2234	0.1581232455326998	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	2169	0.15352252442275108	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	2169	0.15352252442275108	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	2011	0.1423392331093372	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	1978	0.1400034823919786	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1966	0.13915411849475728	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1924	0.13618134485448272	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1833	0.12974033530055448	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	1823	0.12903253205287007	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	1664	0.11777846041468776	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	1655	0.11714143749177179	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	1640	0.11607973262024517	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	1567	0.11091276891214888	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	1549	0.10963872306631692	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	1544	0.1092848214424747	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	1532	0.1084354575452534	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	1487	0.10525034293067351	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	1485	0.10510878228113661	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	1473	0.10425941838391531	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	1457	0.10312693318762023	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	1452	0.10277303156377804	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	1416	0.10022493987211412	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	7.078032476844217E-5	0.0	0.0	0.0	0.0
3	7.078032476844217E-5	0.0	0.0	7.078032476844217E-5	0.0
4	7.078032476844217E-5	0.0	0.0	7.078032476844217E-5	0.0
5	7.078032476844217E-5	0.0	0.0	7.078032476844217E-5	0.0
6	7.078032476844217E-5	0.0	0.0	7.078032476844217E-5	0.0
7	7.078032476844217E-5	0.0	0.0	7.078032476844217E-5	0.0
8	4.954622733790952E-4	0.0	0.0	7.078032476844217E-5	0.0
9	4.954622733790952E-4	0.0	0.0	7.078032476844217E-5	0.0
10	4.954622733790952E-4	0.0	0.0	7.078032476844217E-5	0.0
11	5.662425981475374E-4	0.0	0.0	1.4156064953688434E-4	0.0
12	5.662425981475374E-4	0.0	0.0	2.831212990737687E-4	1.4156064953688434E-4
13	5.662425981475374E-4	0.0	0.0	4.24681948610653E-4	1.4156064953688434E-4
14	5.662425981475374E-4	0.0	0.0	4.24681948610653E-4	1.4156064953688434E-4
15	5.662425981475374E-4	0.0	0.0	8.49363897221306E-4	2.123409743053265E-4
16	5.662425981475374E-4	0.0	0.0	0.0010617048715266324	2.123409743053265E-4
17	5.662425981475374E-4	0.0	0.0	0.0015571671449057278	2.123409743053265E-4
18	6.370229229159795E-4	0.0	0.0	0.002052629418284823	2.123409743053265E-4
19	7.785835724528639E-4	0.0	0.0	0.00261887201643236	2.123409743053265E-4
20	9.909245467581904E-4	0.0	0.0	0.002972773640274571	2.123409743053265E-4
21	9.909245467581904E-4	0.0	0.0	0.005591645656706931	2.123409743053265E-4
22	0.0010617048715266324	0.0	0.0	0.008352078322676176	2.123409743053265E-4
23	0.0010617048715266324	0.0	0.0	0.009838465142813462	2.123409743053265E-4
24	0.0010617048715266324	0.0	0.0	0.014297625603225318	2.123409743053265E-4
25	0.0010617048715266324	0.0	0.0	0.016562595995815468	2.123409743053265E-4
26	0.0010617048715266324	0.0	0.0	0.022012681002985514	2.123409743053265E-4
27	0.0010617048715266324	0.0	0.0	0.05450085007170047	2.123409743053265E-4
28	0.0010617048715266324	0.0	0.0	0.1983972503259434	2.123409743053265E-4
29	0.0010617048715266324	0.0	0.0	0.41569284736506085	2.123409743053265E-4
30	0.0011324851962950747	0.0	0.0	0.7038395494973889	2.123409743053265E-4
31	0.0011324851962950747	0.0	0.0	1.4931109509902876	2.123409743053265E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2475	0.0	30.49697	1
CCCGTTA	35	8.8704063E-4	26.42857	8
GCCTAGT	100	2.0008883E-11	24.050001	1
ACACGAA	195	0.0	20.871796	17
ATACGGC	245	0.0	20.387754	29
GTATCAA	3695	0.0	20.377537	2
TTTTACG	100	1.2903911E-8	20.35	4
TAGCGAA	195	0.0	19.923077	10
AAACACG	215	0.0	19.790697	15
CTTATAC	2095	0.0	19.250597	37
GCGGTAA	1315	0.0	19.13308	23
TGCGGGT	905	0.0	19.01105	21
GGACGTA	205	0.0	18.951221	27
GCAGTCG	395	0.0	18.734177	9
CTAGATA	60	9.238246E-4	18.5	3
GGTCGTG	100	2.876077E-7	18.5	11
TAGAACG	50	0.0070356606	18.5	4
CGTTAGA	160	1.8189894E-12	18.5	1
TCGTGTA	80	1.6170983E-5	18.5	13
GTCGTGT	90	2.1529268E-6	18.5	12
>>END_MODULE