FastQCFastQC Report
Fri 10 Feb 2017
ERR1632719.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632719.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences432504
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA8750.2023102676507038No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA5860.13549007639235708No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA5790.13387159425115144No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA5690.13155947690657196No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC5460.12624160701403916No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA4890.11306253814993619No Hit
TCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT4880.11283132641547824TruSeq Adapter, Index 5 (95% over 21bp)
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT4590.10612618611619776No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA4590.10612618611619776No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG4370.10103952795812293No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3300.025.2272721
TAAACCA502.6983288E-422.1999995
AAGGTTG502.6983288E-422.1999995
GCGGTAA2750.021.52727123
TATACAC1301.8189894E-1221.34615337
ATGTACT1001.2842065E-820.3499984
AAACCAT555.1359687E-420.1818186
CAGTCGG851.2416367E-619.58823610
AGTCGGT851.2416367E-619.58823611
CGGTAAT3050.019.40983624
CATGTAC1153.0340743E-919.3043463
CTTATAC4650.019.09677337
GGTAATA3200.019.07812525
ATTAGAG701.21696765E-418.53
ATCTAAG1103.8351573E-818.532
CGCGGTA3400.018.522
CATCTAA1103.8351573E-818.531
ATCACGC609.224122E-418.524
CTTATTG801.612874E-518.528
TATCTAA609.224122E-418.525
Produced by FastQC (version 0.11.5)