##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632719.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	432504
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.02436278045983	34.0	33.0	34.0	31.0	34.0
2	33.164548767179035	34.0	33.0	34.0	31.0	34.0
3	33.24041164937203	34.0	34.0	34.0	31.0	34.0
4	36.535828570371606	37.0	37.0	37.0	35.0	37.0
5	36.50201847844182	37.0	37.0	37.0	35.0	37.0
6	36.54239035939552	37.0	37.0	37.0	35.0	37.0
7	36.538297911695615	37.0	37.0	37.0	35.0	37.0
8	36.50810165917541	37.0	37.0	37.0	35.0	37.0
9	38.30641797532508	39.0	39.0	39.0	37.0	39.0
10	38.33036226254555	39.0	39.0	39.0	37.0	39.0
11	38.4056471154024	39.0	39.0	39.0	37.0	39.0
12	38.35916430830697	39.0	39.0	39.0	37.0	39.0
13	38.386144405600874	39.0	39.0	39.0	37.0	39.0
14	39.9372537595028	41.0	40.0	41.0	38.0	41.0
15	39.92723997928343	41.0	40.0	41.0	38.0	41.0
16	39.88954784233209	41.0	40.0	41.0	38.0	41.0
17	39.893258790670146	41.0	40.0	41.0	38.0	41.0
18	39.88104387473873	41.0	40.0	41.0	38.0	41.0
19	39.88847039564952	41.0	40.0	41.0	38.0	41.0
20	39.86177700090635	41.0	40.0	41.0	38.0	41.0
21	39.830290586907864	41.0	40.0	41.0	38.0	41.0
22	39.79801805301223	41.0	40.0	41.0	38.0	41.0
23	39.74953064017905	41.0	40.0	41.0	38.0	41.0
24	39.72647883025359	41.0	40.0	41.0	38.0	41.0
25	39.68312431792538	41.0	40.0	41.0	37.0	41.0
26	39.592715905517636	41.0	40.0	41.0	37.0	41.0
27	39.51936860699554	41.0	40.0	41.0	37.0	41.0
28	39.45991019736233	41.0	40.0	41.0	37.0	41.0
29	39.42654403196271	41.0	39.0	41.0	37.0	41.0
30	39.37370290216969	41.0	39.0	41.0	36.0	41.0
31	39.31336820006289	41.0	39.0	41.0	36.0	41.0
32	39.257574496420844	41.0	39.0	41.0	36.0	41.0
33	39.19222481179365	41.0	39.0	41.0	35.0	41.0
34	39.153281819358895	41.0	39.0	41.0	35.0	41.0
35	39.07411723359784	41.0	39.0	41.0	35.0	41.0
36	39.02416162625086	41.0	39.0	41.0	35.0	41.0
37	38.96122810424875	40.0	39.0	41.0	35.0	41.0
38	38.88110167767235	40.0	39.0	41.0	35.0	41.0
39	38.79667008860034	40.0	38.0	41.0	35.0	41.0
40	38.71640031074857	40.0	38.0	41.0	35.0	41.0
41	38.66600540110612	40.0	38.0	41.0	35.0	41.0
42	38.607219817620184	40.0	38.0	41.0	35.0	41.0
43	37.84831122949152	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	3.0
18	6.0
19	24.0
20	32.0
21	62.0
22	133.0
23	177.0
24	292.0
25	437.0
26	550.0
27	766.0
28	1129.0
29	1525.0
30	1898.0
31	2540.0
32	3439.0
33	4547.0
34	6853.0
35	10656.0
36	17579.0
37	33772.0
38	87086.0
39	258997.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.95173223831456	15.409568466418808	11.906710689380908	28.73198860588573
2	21.52211309028356	18.525377799974105	31.25473984055639	28.69776926918595
3	21.290207720622238	18.92583652405527	28.152571999334107	31.631383755988384
4	16.29256608031371	14.824140354771284	32.39600096184081	36.48729260307419
5	17.824575032832065	32.19137857684553	32.82536115272922	17.158685237593176
6	36.808445702236284	32.18929767123541	14.497438173982205	16.504818452546104
7	31.884560605219836	26.574551911658624	19.20120969979468	22.33967778332686
8	28.600429128979155	30.151397443723067	19.66478922738287	21.583384199914914
9	28.29916023898045	12.583698647873776	16.759613783918763	42.35752732922701
10	19.60929841111296	22.977128535227422	29.48966945970442	27.923903593955202
11	40.38066699961156	18.690462978377077	17.96052753269334	22.968342489318015
12	23.89064609807077	22.733893790577657	25.769703864010506	27.605756247341066
13	35.33192756598783	16.691637533988125	20.422007657732646	27.554427242291403
14	25.300806466529792	18.514048424985667	21.99355381684331	34.19159129164123
15	29.855677635351352	23.029151175480457	19.368144572073323	27.74702661709487
16	27.35859090320552	22.761407986978156	21.145700386586018	28.734300723230305
17	27.915811183249172	22.753546788006584	21.021539685182102	28.30910234356214
18	27.170153339622296	21.093908958067438	22.616438229473022	29.119499472837244
19	29.06007805708155	21.48812496531824	22.083263969812997	27.36853300778721
20	30.067698795849285	20.223859201302187	21.862456763405657	27.84598523944287
21	28.11973993304108	21.352403677191425	21.18824334572628	29.33961304404121
22	29.37013299298966	21.90037548785676	20.17576716053493	28.55372435861865
23	28.977997891348984	21.618528383552523	21.287433179808744	28.116040545289756
24	28.88158259808002	21.00373638162884	21.309398294582245	28.805282725708896
25	29.274180123189613	21.12003588406119	21.78754416144128	27.818239831307917
26	28.94562824852487	21.920028485285687	21.420148715387604	27.714194550801842
27	28.363668312894212	21.01460333314836	22.401642528161588	28.22008582579583
28	27.335238518025267	22.113321495292528	21.282346521650666	29.26909346503154
29	28.062168211161055	22.35863714555241	21.34500490168877	28.234189741597763
30	27.590727484601295	21.72927880435788	22.643258790670146	28.036734920370677
31	28.371760723600243	21.716099735493778	21.653672567190128	28.25846697371585
32	26.453396962802657	21.27541016961693	22.378752566450252	29.892440301130165
33	27.056859589737897	21.20974603703087	23.33596914710615	28.39742522612508
34	27.335700941494185	20.781310693080297	23.260594121672863	28.62239424375266
35	26.917670125594213	22.14060447995857	23.876542182268835	27.065183212178383
36	26.995819691841	21.06246416218116	24.200701033978877	27.741015111998962
37	27.077206222370197	20.541312912712947	24.17133714370272	28.210143721214138
38	26.063111555037644	19.944555426076985	25.626815012115493	28.36551800676988
39	25.590514769805594	19.383866970016463	26.420102473040714	28.605515787137232
40	24.9345670791484	20.077733385124763	27.621016221815292	27.36668331391155
41	23.580128738693745	19.385947875626584	28.48158629746777	28.5523370882119
42	22.52441595915876	19.968139022991696	29.26400680687346	28.243438210976084
43	21.89343913582302	20.034728002515585	29.223313541608864	28.848519320052528
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	2.5
2	3.0
3	5.0
4	7.0
5	7.0
6	8.5
7	10.0
8	8.5
9	7.0
10	15.0
11	23.0
12	23.0
13	51.5
14	80.0
15	135.5
16	191.0
17	182.0
18	173.0
19	173.0
20	251.5
21	330.0
22	297.5
23	265.0
24	345.5
25	426.0
26	426.0
27	595.0
28	764.0
29	1094.5
30	1425.0
31	1897.5
32	2370.0
33	2370.0
34	3162.0
35	3954.0
36	4649.0
37	5344.0
38	6967.5
39	8591.0
40	8591.0
41	10356.5
42	12122.0
43	14291.5
44	16461.0
45	21095.0
46	25729.0
47	25729.0
48	29174.5
49	32620.0
50	37181.5
51	41743.0
52	45508.0
53	49273.0
54	49273.0
55	46954.0
56	44635.0
57	43648.5
58	42662.0
59	39407.5
60	36153.0
61	36153.0
62	34190.0
63	32227.0
64	27599.0
65	22971.0
66	20079.0
67	17187.0
68	17187.0
69	14709.5
70	12232.0
71	10585.0
72	8938.0
73	7188.0
74	5438.0
75	5438.0
76	4427.0
77	3416.0
78	2825.0
79	2234.0
80	1717.5
81	1201.0
82	1201.0
83	929.5
84	658.0
85	502.0
86	346.0
87	264.5
88	183.0
89	183.0
90	128.5
91	74.0
92	47.0
93	20.0
94	17.0
95	14.0
96	14.0
97	8.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	432504.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.452373973705875
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.57535723089882	48.873844433058814
2	6.76712063119648	7.640400491949685
3	2.0575173589231133	3.484552182099576
4	1.0521621100745637	2.3758819567557103
5	0.6692318560003849	1.8889863505025504
6	0.44999282108696026	1.52418978128904
7	0.32128269860732717	1.2696019737143585
8	0.2515046117338492	1.135842591816876
9	0.20339692144114402	1.0334015166866342
>10	1.4124801523305972	15.73150446789358
>50	0.15299606098312055	5.958597468394781
>100	0.08490666118771299	8.352641661579073
>500	0.0020508855359350963	0.730555124259314
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	875	0.2023102676507038	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	586	0.13549007639235708	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	579	0.13387159425115144	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	569	0.13155947690657196	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	546	0.12624160701403916	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	489	0.11306253814993619	No Hit
TCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT	488	0.11283132641547824	TruSeq Adapter, Index 5 (95% over 21bp)
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	459	0.10612618611619776	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	459	0.10612618611619776	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	437	0.10103952795812293	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	2.312117344579472E-4	0.0
17	0.0	0.0	0.0	2.312117344579472E-4	0.0
18	0.0	0.0	0.0	6.936352033738416E-4	0.0
19	0.0	0.0	0.0	0.0013872704067476832	0.0
20	0.0	0.0	0.0	0.0018496938756635776	0.0
21	0.0	0.0	0.0	0.0025433290790374194	0.0
22	0.0	0.0	0.0	0.004393022954700997	0.0
23	0.0	0.0	0.0	0.005549081626990733	0.0
24	0.0	0.0	0.0	0.0064739285648225215	0.0
25	0.0	0.0	0.0	0.00739877550265431	0.0
26	2.312117344579472E-4	0.0	0.0	0.009710892847233784	0.0
27	2.312117344579472E-4	0.0	0.0	0.03445054843423413	0.0
28	2.312117344579472E-4	0.0	0.0	0.1317906886410299	0.0
29	2.312117344579472E-4	0.0	0.0	0.29849434918520984	0.0
30	2.312117344579472E-4	0.0	0.0	0.5070473336662782	0.0
31	2.312117344579472E-4	0.0	0.0	1.0656548841166786	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	330	0.0	25.227272	1
TAAACCA	50	2.6983288E-4	22.199999	5
AAGGTTG	50	2.6983288E-4	22.199999	5
GCGGTAA	275	0.0	21.527271	23
TATACAC	130	1.8189894E-12	21.346153	37
ATGTACT	100	1.2842065E-8	20.349998	4
AAACCAT	55	5.1359687E-4	20.181818	6
CAGTCGG	85	1.2416367E-6	19.588236	10
AGTCGGT	85	1.2416367E-6	19.588236	11
CGGTAAT	305	0.0	19.409836	24
CATGTAC	115	3.0340743E-9	19.304346	3
CTTATAC	465	0.0	19.096773	37
GGTAATA	320	0.0	19.078125	25
ATTAGAG	70	1.21696765E-4	18.5	3
ATCTAAG	110	3.8351573E-8	18.5	32
CGCGGTA	340	0.0	18.5	22
CATCTAA	110	3.8351573E-8	18.5	31
ATCACGC	60	9.224122E-4	18.5	24
CTTATTG	80	1.612874E-5	18.5	28
TATCTAA	60	9.224122E-4	18.5	25
>>END_MODULE