FastQCFastQC Report
Fri 10 Feb 2017
ERR1632718.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632718.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32503
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA1810.5568716733839952No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT1280.3938098021721072No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA1240.38150324585422885No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA1180.3630434113774113No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA940.2892040734701412No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA930.2861274343906717No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT910.2799741562317325TruSeq Adapter, Index 10 (95% over 21bp)
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA870.2676675999138541No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA820.25228440451650613No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA760.23382457003968865No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA730.22459465280127988No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT720.2215180137218103No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC710.2184413746423407No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT660.20305817924499278No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC630.19382826200658398No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA620.19075162292711442No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC620.19075162292711442No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC620.19075162292711442No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG620.19075162292711442No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT610.18767498384764483No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC600.18459834476817524No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT590.18152170568870565No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT580.17844506660923606No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC570.17536842752976647No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG570.17536842752976647No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA570.17536842752976647No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA560.17229178845029688No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC560.17229178845029688No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC550.1692151493708273No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC550.1692151493708273No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA540.16613851029135773No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA540.16613851029135773No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC530.16306187121188814No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC510.15690859305294896No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA510.15690859305294896No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC500.15383195397347937No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC480.1476786758145402No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC470.1446020367350706No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA470.1446020367350706No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA460.14152539765560104No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT460.14152539765560104No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA450.13844875857613143No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC450.13844875857613143No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC440.13537211949666184No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA430.13229548041719225No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG430.13229548041719225No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG430.13229548041719225No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT410.12614220225825307No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC410.12614220225825307No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG410.12614220225825307No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA400.12306556317878349No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC400.12306556317878349No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTG400.12306556317878349No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA400.12306556317878349No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG400.12306556317878349No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT390.1199889240993139No Hit
GTTATAACGGTTCATATCACCTTACCGACGCTTATCGCAGATT390.1199889240993139No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG390.1199889240993139No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG390.1199889240993139No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA390.1199889240993139No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG380.11691228501984433No Hit
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTC370.11383564594037474RNA PCR Primer, Index 10 (95% over 22bp)
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC370.11383564594037474No Hit
CCTTACCGACGCTTATCGCAGATTAGCACGTCCTTCATCGCCT370.11383564594037474No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG370.11383564594037474No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC370.11383564594037474No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT360.11075900686090515No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG360.11075900686090515No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA360.11075900686090515No Hit
CCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA350.10768236778143556No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT350.10768236778143556No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT350.10768236778143556No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT340.10460572870196598No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT340.10460572870196598No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG340.10460572870196598No Hit
TGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTA340.10460572870196598No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCCTGT340.10460572870196598No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC330.10152908962249639No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC330.10152908962249639No Hit
GACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCCAGCA330.10152908962249639No Hit
CACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATC330.10152908962249639No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT200.001815748237.036
GTATGCC250.005419087629.59999832
TGCCGTC250.005419087629.59999835
TATGCCG250.005419087629.59999833
TTCTTCT250.005419087629.59999815
CGTATGC250.005419087629.59999831
GCTTCTT250.005419087629.59999813
CTTCTGC250.005419087629.59999817
ATCTCGT358.684558E-426.42857227
GGTATCA808.931238E-1025.43751
TGGTTCA451.2849833E-424.6666665
GTACTGG451.2849833E-424.6666661
TACTGGT451.2849833E-424.6666662
GGTTCAC551.8300167E-523.5454546
CCGTCAT400.001891320823.1251
TTATACA400.001891320823.12537
CTGGTTC502.6246608E-422.1999994
ACTGGTT502.6246608E-422.1999993
GCGGTAA854.80195E-821.76470623
AACTCCG854.80195E-821.7647065
Produced by FastQC (version 0.11.5)