FastQCFastQC Report
Fri 10 Feb 2017
ERR1632717.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632717.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2239774
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA44150.19711810209422914No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA34600.15447987162990554No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA31060.13867470557297298No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA28550.12746821777554343No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA27140.12117293976981605No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC26680.11911916112964968No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA25680.11465442495537496No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA24030.10728761026782166No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT23290.10398370549885837No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG23120.10322470034923167No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA22660.10117092170906529No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGTCG250.00549744329.68
GGTATCA23400.028.6196581
ATACGGC3800.021.90789429
GCGGTAA20500.020.84634223
CTTATAC22750.020.08571437
CGGTAAT21250.019.9364724
TAATACG19900.019.2437227
GTATCAA34650.019.1673872
TATACAC5800.019.1379337
AACTCCG22500.018.9933345
GGTAATA23950.018.38413425
AGCCGCG23800.018.34453819
CTAACTC23200.018.3405173
GTAATAC22900.018.33842726
GCTAACT23500.018.1851042
AATACGG21050.018.10451328
CGTGCCA24400.018.04508210
TCCGTGC24400.017.6659838
TAACTCC24000.017.6520844
TCTTATA42000.017.44285637
Produced by FastQC (version 0.11.5)