##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632717.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2239774
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.03518837168393	34.0	33.0	34.0	31.0	34.0
2	33.17372065217294	34.0	33.0	34.0	31.0	34.0
3	33.25270138862224	34.0	34.0	34.0	31.0	34.0
4	36.53668673714402	37.0	37.0	37.0	35.0	37.0
5	36.510338096611534	37.0	37.0	37.0	35.0	37.0
6	36.55315982773262	37.0	37.0	37.0	35.0	37.0
7	36.54862365577956	37.0	37.0	37.0	35.0	37.0
8	36.52140483816671	37.0	37.0	37.0	35.0	37.0
9	38.31806825152895	39.0	39.0	39.0	37.0	39.0
10	38.34535091486909	39.0	39.0	39.0	37.0	39.0
11	38.41628441083788	39.0	39.0	39.0	37.0	39.0
12	38.37530974107209	39.0	39.0	39.0	37.0	39.0
13	38.40876668806764	39.0	39.0	39.0	37.0	39.0
14	39.96605059260443	41.0	40.0	41.0	38.0	41.0
15	39.955600877588545	41.0	40.0	41.0	38.0	41.0
16	39.92388964243714	41.0	40.0	41.0	38.0	41.0
17	39.916748296926386	41.0	40.0	41.0	38.0	41.0
18	39.91826720017288	41.0	40.0	41.0	38.0	41.0
19	39.92718685010184	41.0	40.0	41.0	38.0	41.0
20	39.8917292548266	41.0	40.0	41.0	38.0	41.0
21	39.86615078128418	41.0	40.0	41.0	38.0	41.0
22	39.83489896748511	41.0	40.0	41.0	38.0	41.0
23	39.8067715760608	41.0	40.0	41.0	38.0	41.0
24	39.79157941828059	41.0	40.0	41.0	38.0	41.0
25	39.73456071907255	41.0	40.0	41.0	38.0	41.0
26	39.658694136104806	41.0	40.0	41.0	37.0	41.0
27	39.583646832225035	41.0	40.0	41.0	37.0	41.0
28	39.54265430351455	41.0	40.0	41.0	37.0	41.0
29	39.50878883315906	41.0	40.0	41.0	37.0	41.0
30	39.455942429905875	41.0	39.0	41.0	37.0	41.0
31	39.4012083361982	41.0	39.0	41.0	36.0	41.0
32	39.35359371079404	41.0	39.0	41.0	36.0	41.0
33	39.295441415071345	41.0	39.0	41.0	36.0	41.0
34	39.255271290764156	41.0	39.0	41.0	36.0	41.0
35	39.17735762626051	41.0	39.0	41.0	35.0	41.0
36	39.1328008986621	41.0	39.0	41.0	35.0	41.0
37	39.08262931885092	41.0	39.0	41.0	35.0	41.0
38	39.0086464080751	41.0	39.0	41.0	35.0	41.0
39	38.92987863954131	40.0	39.0	41.0	35.0	41.0
40	38.85112113990072	40.0	39.0	41.0	35.0	41.0
41	38.79960612097471	40.0	38.0	41.0	35.0	41.0
42	38.733943246059646	40.0	38.0	41.0	35.0	41.0
43	37.998408321553875	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	2.0
12	1.0
13	1.0
14	1.0
15	1.0
16	1.0
17	7.0
18	24.0
19	49.0
20	106.0
21	235.0
22	426.0
23	720.0
24	1217.0
25	1775.0
26	2527.0
27	3575.0
28	5152.0
29	7070.0
30	9556.0
31	12651.0
32	16768.0
33	22955.0
34	34212.0
35	53236.0
36	84936.0
37	162813.0
38	438921.0
39	1380834.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.352811489016304	16.646099115357174	11.929016052512441	28.072073343114084
2	21.22379311484105	18.70349419182471	32.503457938166974	27.56925475516726
3	20.917378271200576	19.86905821748087	28.694323623722752	30.519239887595802
4	15.35355799290464	15.444995789753788	34.19344987485344	35.00799634248813
5	16.983677817494087	32.93309950021743	33.68692555588198	16.396297126406502
6	34.95660722912222	34.083706659689774	14.814798278754909	16.144887832433096
7	30.75828186236647	27.378208694270047	19.868343859692985	21.995165583670495
8	27.25038329760056	31.074920951846035	19.582020328836748	22.092675421716656
9	27.67971232811882	12.894917076455034	17.750540902787513	41.67482969263863
10	19.349452221518778	23.061433876810785	30.11875305276336	27.470360848907077
11	39.36464125398366	19.490761121434574	19.01325758759589	22.131340036985875
12	23.21444038550318	23.57300334765918	26.480618133793854	26.73193813304378
13	34.074062829553334	17.89207303951202	20.74713788087548	27.286726250059157
14	24.076223761861687	19.3825805639319	22.74430366635205	33.79689200785436
15	29.23643189000319	23.895759125697502	20.429784433608035	26.438024550691274
16	26.369535497777903	23.57152998472167	22.32694012878085	27.731994388719578
17	26.702024400676137	23.431426563572934	21.99083478958145	27.87571424616948
18	26.08593545598797	22.082897649494992	23.652654241008246	28.17851265350879
19	28.03742699040171	22.25773671807959	23.16894472388732	26.535891567631374
20	28.840543733430245	21.366128904076927	23.015536388939243	26.777790973553582
21	27.361019459999092	22.398599144377958	22.158173101393267	28.082208294229687
22	28.200211271315766	23.11773419996839	21.28491535306687	27.39713917564897
23	28.07944015780163	22.542051117657408	22.43369197070776	26.9448167538332
24	28.134043881213017	22.09714015789093	21.74255081093003	28.026265149966022
25	28.21423054290299	22.118035123186537	22.799621747551317	26.86811258635916
26	27.90513685755795	22.728498500295117	22.464855829204197	26.901508812942737
27	27.380976830698096	22.148707860703805	23.230022314751402	27.240292993846698
28	26.318816094838137	22.980175678438986	22.557633046905625	28.143375179817248
29	27.70493808750347	23.348114586560968	22.510485432905284	26.436461893030277
30	27.0309861619967	22.018248269691494	23.770032155029927	27.180733413281878
31	27.296950495898248	22.599869451114262	22.92985810175491	27.17332195123258
32	25.588206667279824	22.295642328199182	23.14407614339661	28.97207486112438
33	26.08044383049361	21.97199360292601	24.400363608114034	27.547198958466346
34	26.4926729214644	21.594544806752825	24.348215489598505	27.564566782184276
35	26.075934446957593	23.059380098170617	24.1986468277603	26.666038627111487
36	26.277606579949587	21.898771929667905	24.796475001495686	27.027146488886828
37	26.17143515372533	21.336170524347544	24.92782753974285	27.564566782184276
38	25.493688202470427	20.695659472786094	26.070487468824982	27.740164855918497
39	24.745755598555927	20.19355524262716	27.19604745835964	27.864641700457277
40	24.626815026873246	20.957025128428135	27.92192426557322	26.49423557912539
41	23.309271381844777	20.090330542277925	28.721737103832794	27.8786609720445
42	21.785992693905726	21.11815745695771	29.615041517581687	27.48080833155488
43	21.553112055055553	20.984974376879094	29.165531879555708	28.29638168850964
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5.0
1	7.5
2	10.0
3	37.0
4	64.0
5	64.0
6	73.5
7	83.0
8	83.0
9	83.0
10	126.5
11	170.0
12	170.0
13	358.5
14	547.0
15	951.5
16	1356.0
17	1339.5
18	1323.0
19	1323.0
20	1631.0
21	1939.0
22	1712.5
23	1486.0
24	1957.5
25	2429.0
26	2429.0
27	3199.0
28	3969.0
29	5851.5
30	7734.0
31	11179.0
32	14624.0
33	14624.0
34	21245.0
35	27866.0
36	33295.0
37	38724.0
38	52083.5
39	65443.0
40	65443.0
41	75930.0
42	86417.0
43	103279.5
44	120142.0
45	139626.0
46	159110.0
47	159110.0
48	179485.0
49	199860.0
50	218689.5
51	237519.0
52	242460.0
53	247401.0
54	247401.0
55	233670.5
56	219940.0
57	207823.0
58	195706.0
59	182910.0
60	170114.0
61	170114.0
62	154884.0
63	139654.0
64	117282.0
65	94910.0
66	82209.5
67	69509.0
68	69509.0
69	59172.0
70	48835.0
71	40736.5
72	32638.0
73	26246.5
74	19855.0
75	19855.0
76	16206.0
77	12557.0
78	10087.0
79	7617.0
80	6194.5
81	4772.0
82	4772.0
83	3757.5
84	2743.0
85	2084.0
86	1425.0
87	1052.0
88	679.0
89	679.0
90	500.5
91	322.0
92	223.0
93	124.0
94	89.0
95	54.0
96	54.0
97	33.0
98	12.0
99	8.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2239774.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.64348780059572
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.93010603846248	34.53498859114822
2	8.933369654631475	7.4403334046171565
3	2.720658725335751	3.3989315531431084
4	1.2328283894830412	2.053570959906604
5	0.7733280603129846	1.6102038822751061
6	0.5218100492553713	1.3037994252236584
7	0.37194664215878503	1.0842408818648335
8	0.2749367851573755	0.9159461326908938
9	0.2280413729162966	0.8546794317963806
>10	1.5850444785483593	13.151430134056206
>50	0.21701908974636513	6.262785364721421
>100	0.1800288914497177	15.10314298477328
>500	0.020982422981511987	5.971555664365318
>1k	0.00989939956050822	6.3143915894179115
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	4415	0.19711810209422914	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	3460	0.15447987162990554	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	3106	0.13867470557297298	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	2855	0.12746821777554343	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	2714	0.12117293976981605	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	2668	0.11911916112964968	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	2568	0.11465442495537496	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	2403	0.10728761026782166	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	2329	0.10398370549885837	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	2312	0.10322470034923167	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	2266	0.10117092170906529	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	4.464736174274726E-5	0.0	0.0	0.0	0.0
4	4.464736174274726E-5	0.0	0.0	0.0	0.0
5	4.464736174274726E-5	0.0	0.0	0.0	0.0
6	4.464736174274726E-5	0.0	0.0	0.0	4.464736174274726E-5
7	4.464736174274726E-5	0.0	0.0	4.464736174274726E-5	4.464736174274726E-5
8	1.3394208522824177E-4	0.0	0.0	4.464736174274726E-5	4.464736174274726E-5
9	1.3394208522824177E-4	0.0	0.0	4.464736174274726E-5	4.464736174274726E-5
10	1.3394208522824177E-4	0.0	0.0	4.464736174274726E-5	4.464736174274726E-5
11	1.3394208522824177E-4	0.0	0.0	4.464736174274726E-5	4.464736174274726E-5
12	1.3394208522824177E-4	0.0	0.0	4.464736174274726E-5	4.464736174274726E-5
13	1.3394208522824177E-4	0.0	0.0	4.464736174274726E-5	8.929472348549453E-5
14	1.3394208522824177E-4	0.0	0.0	8.929472348549453E-5	8.929472348549453E-5
15	1.3394208522824177E-4	0.0	0.0	8.929472348549453E-5	8.929472348549453E-5
16	1.7858944697098905E-4	0.0	0.0	2.6788417045648354E-4	1.3394208522824177E-4
17	2.232368087137363E-4	0.0	0.0	5.804157026557144E-4	1.3394208522824177E-4
18	2.232368087137363E-4	0.0	0.0	6.250630643984616E-4	1.3394208522824177E-4
19	2.232368087137363E-4	0.0	0.0	9.375945965976924E-4	1.3394208522824177E-4
20	2.232368087137363E-4	0.0	0.0	0.0014287155757679124	1.3394208522824177E-4
21	2.232368087137363E-4	0.0	0.0	0.002455604895851099	1.7858944697098905E-4
22	2.232368087137363E-4	0.0	0.0	0.003303904768963297	3.125315321992308E-4
23	2.232368087137363E-4	0.0	0.0	0.003973615195104506	3.125315321992308E-4
24	2.232368087137363E-4	0.0	0.0	0.00553627285610066	3.125315321992308E-4
25	2.232368087137363E-4	0.0	0.0	0.006563162176183847	3.125315321992308E-4
26	2.232368087137363E-4	0.0	0.0	0.009867066945147144	3.125315321992308E-4
27	2.232368087137363E-4	0.0	0.0	0.03161033211386506	3.125315321992308E-4
28	2.232368087137363E-4	0.0	0.0	0.12010140308799012	3.125315321992308E-4
29	2.232368087137363E-4	0.0	0.0	0.26087453466287225	3.125315321992308E-4
30	2.232368087137363E-4	0.0	0.0	0.4491971064937802	3.125315321992308E-4
31	2.232368087137363E-4	0.0	0.0	1.0134058168368774	3.125315321992308E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGTCG	25	0.005497443	29.6	8
GGTATCA	2340	0.0	28.619658	1
ATACGGC	380	0.0	21.907894	29
GCGGTAA	2050	0.0	20.846342	23
CTTATAC	2275	0.0	20.085714	37
CGGTAAT	2125	0.0	19.93647	24
TAATACG	1990	0.0	19.24372	27
GTATCAA	3465	0.0	19.167387	2
TATACAC	580	0.0	19.13793	37
AACTCCG	2250	0.0	18.993334	5
GGTAATA	2395	0.0	18.384134	25
AGCCGCG	2380	0.0	18.344538	19
CTAACTC	2320	0.0	18.340517	3
GTAATAC	2290	0.0	18.338427	26
GCTAACT	2350	0.0	18.185104	2
AATACGG	2105	0.0	18.104513	28
CGTGCCA	2440	0.0	18.045082	10
TCCGTGC	2440	0.0	17.665983	8
TAACTCC	2400	0.0	17.652084	4
TCTTATA	4200	0.0	17.442856	37
>>END_MODULE