FastQCFastQC Report
Fri 10 Feb 2017
ERR1632716.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632716.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences196721
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA3480.17690028009211015No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA2520.12810020282532114No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA2130.1082751714356881No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA2110.10725850315929668No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT1990.10115849350094805No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGACCG200.001838230137.05
ATACGGC352.3783297E-531.71428729
TAACGTC556.217506E-726.9090927
ACACATA358.8431744E-426.4285728
TAACACC358.8431744E-426.42857235
ATTGCCG358.8431744E-426.42857211
GGTATCA1750.025.371431
TACGGCT451.3177725E-424.66666630
GGACCGT451.3177725E-424.6666666
GTAACGT601.3280132E-624.66666626
GAAACAT551.8922856E-523.54545427
ACCGTTA551.8922856E-523.5454548
TCTAGCA400.001925532723.1253
GTCAATG652.664121E-622.7692331
CGTCAAT652.664121E-622.7692330
GCTTAGG502.691084E-422.21
ACTGAAA603.7061138E-521.58333224
TGAAACA603.7061138E-521.58333226
TATACAC603.7061138E-521.58333237
TCTTATA4400.021.44318237
Produced by FastQC (version 0.11.5)