##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632716.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	196721
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.02243786885996	34.0	33.0	34.0	31.0	34.0
2	33.16661159713503	34.0	33.0	34.0	31.0	34.0
3	33.245606722210646	34.0	34.0	34.0	31.0	34.0
4	36.53925102048078	37.0	37.0	37.0	35.0	37.0
5	36.50970155702746	37.0	37.0	37.0	35.0	37.0
6	36.546149114736096	37.0	37.0	37.0	35.0	37.0
7	36.54405477808673	37.0	37.0	37.0	35.0	37.0
8	36.5210170749437	37.0	37.0	37.0	35.0	37.0
9	38.30778107065336	39.0	39.0	39.0	37.0	39.0
10	38.343888044489404	39.0	39.0	39.0	37.0	39.0
11	38.40941231490283	39.0	39.0	39.0	37.0	39.0
12	38.37459650977781	39.0	39.0	39.0	37.0	39.0
13	38.407592478688095	39.0	39.0	39.0	37.0	39.0
14	39.96715653133118	41.0	40.0	41.0	38.0	41.0
15	39.949710503708296	41.0	40.0	41.0	38.0	41.0
16	39.91169727685402	41.0	40.0	41.0	38.0	41.0
17	39.92347029549463	41.0	40.0	41.0	38.0	41.0
18	39.91090935893982	41.0	40.0	41.0	38.0	41.0
19	39.922672210897666	41.0	40.0	41.0	38.0	41.0
20	39.89091657728458	41.0	40.0	41.0	38.0	41.0
21	39.858820359798905	41.0	40.0	41.0	38.0	41.0
22	39.833134235795875	41.0	40.0	41.0	38.0	41.0
23	39.793072422364666	41.0	40.0	41.0	38.0	41.0
24	39.775189227382945	41.0	40.0	41.0	38.0	41.0
25	39.72503189796717	41.0	40.0	41.0	38.0	41.0
26	39.64651460698146	41.0	40.0	41.0	37.0	41.0
27	39.57219107263587	41.0	40.0	41.0	37.0	41.0
28	39.52883525398915	41.0	40.0	41.0	37.0	41.0
29	39.50078537624351	41.0	40.0	41.0	37.0	41.0
30	39.44127469868494	41.0	39.0	41.0	37.0	41.0
31	39.38171318771255	41.0	39.0	41.0	36.0	41.0
32	39.333035110638924	41.0	39.0	41.0	36.0	41.0
33	39.27068792858922	41.0	39.0	41.0	36.0	41.0
34	39.22802344437045	41.0	39.0	41.0	35.0	41.0
35	39.16194000640501	41.0	39.0	41.0	35.0	41.0
36	39.12079035791807	41.0	39.0	41.0	35.0	41.0
37	39.072239364378994	41.0	39.0	41.0	35.0	41.0
38	38.99577065997021	41.0	39.0	41.0	35.0	41.0
39	38.911260109495174	40.0	39.0	41.0	35.0	41.0
40	38.83807524361914	40.0	39.0	41.0	35.0	41.0
41	38.78985466726989	40.0	38.0	41.0	35.0	41.0
42	38.734827496810205	40.0	38.0	41.0	35.0	41.0
43	38.02477620589566	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	1.0
19	11.0
20	15.0
21	27.0
22	54.0
23	67.0
24	124.0
25	176.0
26	225.0
27	329.0
28	436.0
29	587.0
30	889.0
31	1123.0
32	1446.0
33	2035.0
34	3032.0
35	4755.0
36	7679.0
37	14476.0
38	37828.0
39	121404.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.245510138724384	16.061325430431932	12.58025325206765	28.112911178776034
2	20.991149902654012	18.91917995536826	32.4266346755049	27.663035466472824
3	20.97589987850814	19.478347507383553	28.883037398142548	30.662715215965758
4	16.15638391427453	15.174790693418597	32.73265182669873	35.93617356560815
5	17.121202108570007	33.07323569928985	33.098144072061444	16.70741812007869
6	35.9417652411283	32.86431036849142	14.758465034236304	16.43545935614398
7	31.159866003121174	27.192826388641784	19.589672683648416	22.057634924588633
8	27.884669150726154	31.297116220434017	19.52155590913019	21.29665871970964
9	27.665068803025605	13.264979336217284	17.164410510316642	41.90554135044047
10	19.24603880622811	23.646687440588448	29.890555660046463	27.216718093136983
11	39.78070465278237	19.07828854062352	18.859704861199365	22.281301945394745
12	23.53129559121802	23.32694526766334	26.073474616334806	27.06828452478383
13	34.277479272675514	17.520244407053646	21.213291921045542	26.988984399225295
14	24.318705171283188	19.009155097828906	22.526827334143277	34.14531239674463
15	29.234296287635793	23.996421327667104	20.11630685081918	26.652975533877925
16	27.077434539271355	23.37676201320652	21.759242785467745	27.786560662054384
17	27.20045140071472	23.31271191179386	21.72416772993224	27.762668957559182
18	26.453200217567012	21.82024288205123	23.562812307786153	28.163744592595606
19	28.678687074587867	22.004768174216277	22.858769526385085	26.45777522481077
20	29.36697149770487	20.834074653951536	22.86232786535245	26.936625982991142
21	27.552726958484353	22.179635117755602	21.762292790296918	28.505345133463127
22	28.322853177850867	22.4887022737786	21.28191702970196	27.90652751866857
23	28.13934455396221	22.440918864788202	22.106943335993616	27.312793245255975
24	28.3182781706071	21.791267836174075	21.886834654154868	28.003619339063953
25	28.56939523487579	22.0373015590608	22.46379390100701	26.9295093050564
26	28.307094819566796	22.253343567793983	22.08762663874218	27.35193497389704
27	27.566451980215632	21.616400892634747	23.191220052765082	27.62592707438453
28	26.775484061183096	22.425160506504135	22.47345224963273	28.32590318268004
29	27.64626043991236	23.200370067252607	22.06729327321435	27.08607621962068
30	27.1785930327723	22.393643789936	23.37676201320652	27.051001164085175
31	27.731152240991047	22.02916821284967	22.67729423904921	27.56238530711007
32	25.711540709939456	22.159301752227776	23.19986173311441	28.929295804718357
33	26.51979198967065	21.64232593368273	24.082838131160376	27.755043945486246
34	26.766334046695572	21.41205056908007	23.94457124557114	27.87704413865322
35	26.19242480467261	22.36416040992065	24.396988628565328	27.046426156841413
36	26.017049526995084	21.352067140772974	25.165589845517257	27.465293486714685
37	26.507083636215757	20.335398864381535	25.16863985034643	27.988877649056278
38	25.490923693962515	19.894673166565845	26.6682255580238	27.94617758144784
39	24.496113785513494	19.275522186243464	27.824177388280862	28.40418663996218
40	24.455447054457835	20.031415049740495	29.048754327194352	26.464383568607317
41	22.829286146369732	19.563239308462236	29.768555466879487	27.838919078288544
42	21.36426716008967	20.0309067156023	30.77963206775077	27.825194056557258
43	20.759349535636765	20.407582312005328	30.433456519639492	28.39961163271842
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	1.5
2	2.0
3	4.0
4	6.0
5	6.0
6	7.0
7	8.0
8	10.5
9	13.0
10	18.5
11	24.0
12	24.0
13	47.0
14	70.0
15	117.0
16	164.0
17	153.0
18	142.0
19	142.0
20	186.5
21	231.0
22	232.0
23	233.0
24	288.0
25	343.0
26	343.0
27	415.0
28	487.0
29	721.5
30	956.0
31	1211.0
32	1466.0
33	1466.0
34	1928.0
35	2390.0
36	2788.5
37	3187.0
38	4241.0
39	5295.0
40	5295.0
41	6237.0
42	7179.0
43	8043.5
44	8908.0
45	10954.5
46	13001.0
47	13001.0
48	14659.5
49	16318.0
50	18363.5
51	20409.0
52	21601.0
53	22793.0
54	22793.0
55	21080.0
56	19367.0
57	18380.0
58	17393.0
59	16084.5
60	14776.0
61	14776.0
62	13700.0
63	12624.0
64	10810.0
65	8996.0
66	7781.0
67	6566.0
68	6566.0
69	5624.5
70	4683.0
71	4063.0
72	3443.0
73	2773.0
74	2103.0
75	2103.0
76	1708.5
77	1314.0
78	1061.0
79	808.0
80	661.5
81	515.0
82	515.0
83	380.5
84	246.0
85	193.0
86	140.0
87	102.0
88	64.0
89	64.0
90	49.5
91	35.0
92	24.5
93	14.0
94	10.5
95	7.0
96	7.0
97	4.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	196721.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.80140624589455
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.7251962527198	48.12510291613879
2	7.244368264417485	8.229806095640871
3	2.333469111285241	3.976329808570784
4	1.2691032487568426	2.883473968024879
5	0.8388624067020716	2.382428217374659
6	0.5814022252417851	1.981467839293543
7	0.43977475318471526	1.7485877088633115
8	0.3639832939226419	1.6539810355855056
9	0.24730886010927217	1.2642741928148284
>10	1.7740416362218958	19.027547198072885
>50	0.14160422150945376	5.3516392310877725
>100	0.04088572592878595	3.3753617885321803
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	348	0.17690028009211015	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	252	0.12810020282532114	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	213	0.1082751714356881	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	211	0.10725850315929668	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	199	0.10115849350094805	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	5.083341381957188E-4	0.0	0.0	0.0	0.0
3	5.083341381957188E-4	0.0	0.0	0.0	0.0
4	5.083341381957188E-4	0.0	0.0	0.0	0.0
5	5.083341381957188E-4	0.0	0.0	0.0	0.0
6	5.083341381957188E-4	0.0	0.0	0.0	0.0
7	5.083341381957188E-4	0.0	0.0	0.0	0.0
8	5.083341381957188E-4	0.0	0.0	0.0	0.0
9	5.083341381957188E-4	0.0	0.0	0.0	0.0
10	5.083341381957188E-4	0.0	0.0	0.0	0.0
11	5.083341381957188E-4	0.0	0.0	0.0	0.0
12	5.083341381957188E-4	0.0	0.0	0.0	0.0
13	5.083341381957188E-4	0.0	0.0	0.0	0.0
14	5.083341381957188E-4	0.0	0.0	0.0	0.0
15	5.083341381957188E-4	0.0	0.0	5.083341381957188E-4	0.0
16	5.083341381957188E-4	0.0	0.0	5.083341381957188E-4	0.0
17	5.083341381957188E-4	0.0	0.0	0.0010166682763914376	0.0
18	5.083341381957188E-4	0.0	0.0	0.0015250024145871564	0.0
19	5.083341381957188E-4	0.0	0.0	0.0015250024145871564	0.0
20	5.083341381957188E-4	0.0	0.0	0.002033336552782875	0.0
21	5.083341381957188E-4	0.0	0.0	0.003050004829174313	0.0
22	5.083341381957188E-4	0.0	0.0	0.005591675520152907	0.0
23	5.083341381957188E-4	0.0	0.0	0.006100009658348626	0.0
24	5.083341381957188E-4	0.0	0.0	0.0081333462111315	0.0
25	5.083341381957188E-4	0.0	0.0	0.009150014487522938	0.0
26	5.083341381957188E-4	0.0	0.0	0.012708353454892971	0.0
27	5.083341381957188E-4	0.0	0.0	0.04270006760844038	0.0
28	5.083341381957188E-4	0.0	0.0	0.17486694353932727	0.0
29	5.083341381957188E-4	0.0	0.0	0.37362559157385333	0.0
30	5.083341381957188E-4	0.0	0.0	0.6293176630862999	0.0
31	5.083341381957188E-4	0.0	0.0	1.3674188317464837	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGACCG	20	0.0018382301	37.0	5
ATACGGC	35	2.3783297E-5	31.714287	29
TAACGTC	55	6.217506E-7	26.90909	27
ACACATA	35	8.8431744E-4	26.428572	8
TAACACC	35	8.8431744E-4	26.428572	35
ATTGCCG	35	8.8431744E-4	26.428572	11
GGTATCA	175	0.0	25.37143	1
TACGGCT	45	1.3177725E-4	24.666666	30
GGACCGT	45	1.3177725E-4	24.666666	6
GTAACGT	60	1.3280132E-6	24.666666	26
GAAACAT	55	1.8922856E-5	23.545454	27
ACCGTTA	55	1.8922856E-5	23.545454	8
TCTAGCA	40	0.0019255327	23.125	3
GTCAATG	65	2.664121E-6	22.76923	31
CGTCAAT	65	2.664121E-6	22.76923	30
GCTTAGG	50	2.691084E-4	22.2	1
ACTGAAA	60	3.7061138E-5	21.583332	24
TGAAACA	60	3.7061138E-5	21.583332	26
TATACAC	60	3.7061138E-5	21.583332	37
TCTTATA	440	0.0	21.443182	37
>>END_MODULE