FastQCFastQC Report
Fri 10 Feb 2017
ERR1632715.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632715.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1128927
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA21790.19301513738266513No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA18720.1658211735568376No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA16240.14385341124802578No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA14630.12959208168464392No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT11840.10487834908723061No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA11790.10443545065358523No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA11690.10354965378629442No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15550.029.6237951
TATATCT753.7406426E-722.1999995
TACCTTA603.7262114E-521.58333231
GACCGTT1151.3460522E-1020.9130427
AACCGTC656.901993E-519.92307716
CCCGTAT656.901993E-519.9230771
GTATCAA23250.019.6537632
ATACGGC2000.019.42529
GTACTAT1052.2593667E-819.380951
TATACAC7400.019.2537
CTTATAC24050.019.07692337
ATACACA4000.018.962537
CGTTATT609.2366786E-418.510
ATTGTAC500.00703480818.4999983
ATAGTAC500.00703480818.4999983
GGACCGT2800.017.8392856
GTTTATA1156.404662E-817.695651
GCGGTAA11100.017.523
CGCGGTA11650.017.30901122
TTTTACG752.0672257E-417.2666664
Produced by FastQC (version 0.11.5)