##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632715.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1128927
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.00558937823261	34.0	33.0	34.0	31.0	34.0
2	33.14262569678996	34.0	33.0	34.0	31.0	34.0
3	33.21902479079692	34.0	33.0	34.0	31.0	34.0
4	36.515459369826395	37.0	37.0	37.0	35.0	37.0
5	36.48381339094556	37.0	37.0	37.0	35.0	37.0
6	36.5269711859137	37.0	37.0	37.0	35.0	37.0
7	36.526865776086495	37.0	37.0	37.0	35.0	37.0
8	36.50035741903595	37.0	37.0	37.0	35.0	37.0
9	38.28852529880143	39.0	39.0	39.0	37.0	39.0
10	38.326285933457164	39.0	39.0	39.0	37.0	39.0
11	38.39046103069552	39.0	39.0	39.0	37.0	39.0
12	38.352636618665336	39.0	39.0	39.0	37.0	39.0
13	38.38006709025473	39.0	39.0	39.0	37.0	39.0
14	39.92085227831383	41.0	40.0	41.0	38.0	41.0
15	39.90699664371567	41.0	40.0	41.0	38.0	41.0
16	39.870901307170435	41.0	40.0	41.0	38.0	41.0
17	39.87350732155401	41.0	40.0	41.0	38.0	41.0
18	39.87750137962862	41.0	40.0	41.0	38.0	41.0
19	39.884570038629604	41.0	40.0	41.0	38.0	41.0
20	39.85499328123076	41.0	40.0	41.0	38.0	41.0
21	39.82707119238002	41.0	40.0	41.0	38.0	41.0
22	39.79078186632085	41.0	40.0	41.0	38.0	41.0
23	39.754209971061016	41.0	40.0	41.0	38.0	41.0
24	39.732863152356174	41.0	40.0	41.0	38.0	41.0
25	39.69304835476519	41.0	40.0	41.0	37.0	41.0
26	39.60990037442634	41.0	40.0	41.0	37.0	41.0
27	39.52651322893332	41.0	40.0	41.0	37.0	41.0
28	39.4836796356186	41.0	39.0	41.0	37.0	41.0
29	39.45278392668436	41.0	39.0	41.0	37.0	41.0
30	39.400146333642475	41.0	39.0	41.0	36.0	41.0
31	39.34797732714338	41.0	39.0	41.0	36.0	41.0
32	39.29671891982387	41.0	39.0	41.0	36.0	41.0
33	39.235699031026805	41.0	39.0	41.0	36.0	41.0
34	39.19794814013661	41.0	39.0	41.0	35.0	41.0
35	39.12444914507315	41.0	39.0	41.0	35.0	41.0
36	39.07736018360797	41.0	39.0	41.0	35.0	41.0
37	39.02765103500935	41.0	39.0	41.0	35.0	41.0
38	38.95920196788632	41.0	39.0	41.0	35.0	41.0
39	38.88427506827279	40.0	39.0	41.0	35.0	41.0
40	38.80726123123993	40.0	38.0	41.0	35.0	41.0
41	38.75000155014452	40.0	38.0	41.0	35.0	41.0
42	38.68107060952568	40.0	38.0	41.0	35.0	41.0
43	37.93958953944763	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	3.0
15	0.0
16	5.0
17	11.0
18	14.0
19	22.0
20	83.0
21	143.0
22	241.0
23	424.0
24	651.0
25	984.0
26	1373.0
27	2069.0
28	2750.0
29	3794.0
30	5032.0
31	6857.0
32	9096.0
33	12239.0
34	18039.0
35	28314.0
36	44062.0
37	85356.0
38	222310.0
39	685054.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.319630055796345	16.4348093366533	12.663883492909639	27.58167711464072
2	21.155398001819425	19.246771491867943	32.7349775494784	26.86285295683423
3	21.455240241397362	20.037876674045354	28.747562951368867	29.759320133188417
4	15.90705156312144	15.748936822310034	33.60996769498825	34.73404391958027
5	17.58324497509582	32.65764748296392	32.651269745519414	17.10783779642085
6	34.41285397550063	33.40490572021043	15.16493094770521	17.01730935658373
7	30.210190738639437	27.69691928707525	19.754864574945945	22.338025399339372
8	27.12974355294895	31.166674195940036	19.8759530067046	21.827629244406413
9	27.694084737099917	13.397146139652962	17.859436438317093	41.04933268493003
10	19.70322261758289	23.370687387226987	29.642040627959116	27.284049367231006
11	38.71437214275148	19.904121347084445	19.003885990856805	22.37762051930727
12	22.961714973598824	23.72544903257695	26.630685597917314	26.682150395906913
13	34.45351205170928	18.01852555568252	21.030412063844693	26.49755032876351
14	24.215206120502035	19.92768354375438	23.11141464417097	32.74569569157262
15	29.312967091760584	24.012978695699545	20.305475907653907	26.368578304885965
16	25.745331629060157	23.901368290420905	22.502606457282003	27.850693623236932
17	26.56106196414826	23.495850484575175	22.302416365274283	27.640671186002287
18	26.396923804639272	21.7980436290389	23.66796081588978	28.137071750432046
19	28.253199719733875	22.42129030486471	23.239943769614865	26.08556620578656
20	29.21703529103299	21.3371635189875	22.996792529543537	26.449008660435975
21	27.467232159386747	22.46053110608569	22.155639824364197	27.916596910163367
22	27.847947652948328	23.152869937560176	21.682978615977827	27.316203793513665
23	27.960798173841177	22.40073981754356	22.569927019196104	27.068534989419156
24	27.70958618227751	21.968559525992383	22.53192633358933	27.78992795814078
25	28.02891595293584	22.483296085575063	22.892799977323598	26.594987984165495
26	27.604087775383174	22.90608693033296	22.60969929853746	26.8801259957464
27	27.265270473644442	22.232261253384852	23.71287071706142	26.789597555909282
28	26.40223858584302	23.19423665126266	22.555488530259264	27.84803623263506
29	27.004934774347678	23.558210584032448	22.533520767950453	26.90333387366942
30	26.784902832512643	22.660278299659765	23.929270891740565	26.625547976087027
31	27.385207369475616	22.301353409033535	22.925485881726633	27.38795333976422
32	25.02349576190489	22.367433855333427	23.6939146641014	28.915155718660284
33	25.812386451914076	21.919309220171012	24.728702564470513	27.539601763444406
34	25.962263281859677	21.436549927497524	24.930752829899543	27.670433960743257
35	25.782269358426184	22.313931724549064	24.993201509043544	26.910597407981207
36	25.571804022757895	21.04821658087724	25.938346766442827	27.44163262992204
37	25.658169217318743	20.286962753127526	25.76225034922541	28.292617680328313
38	24.536661803641866	19.660350049206016	27.627118493932734	28.175869653219383
39	23.928296515186545	18.81255386752199	29.02295719740958	28.23619241988189
40	23.654053805073314	19.761596631137355	29.883951752416234	26.7003978113731
41	22.1285344402251	19.28831536494388	30.81988472239569	27.76326547243533
42	20.539592019678864	20.30875335606288	31.46235319024171	27.68930143401655
43	19.738654492274524	20.809140006395456	31.005459166093114	28.44674633523691
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	16.0
1	15.5
2	15.0
3	40.5
4	66.0
5	66.0
6	82.0
7	98.0
8	109.0
9	120.0
10	180.5
11	241.0
12	241.0
13	438.0
14	635.0
15	1129.5
16	1624.0
17	1593.0
18	1562.0
19	1562.0
20	1892.0
21	2222.0
22	2079.5
23	1937.0
24	2395.5
25	2854.0
26	2854.0
27	3413.5
28	3973.0
29	5491.0
30	7009.0
31	8487.0
32	9965.0
33	9965.0
34	12788.5
35	15612.0
36	17665.0
37	19718.0
38	24779.5
39	29841.0
40	29841.0
41	35653.5
42	41466.0
43	48004.5
44	54543.0
45	67191.0
46	79839.0
47	79839.0
48	90607.0
49	101375.0
50	109764.0
51	118153.0
52	122021.0
53	125889.0
54	125889.0
55	118711.0
56	111533.0
57	105083.0
58	98633.0
59	91629.5
60	84626.0
61	84626.0
62	77375.5
63	70125.0
64	58370.0
65	46615.0
66	40142.0
67	33669.0
68	33669.0
69	28785.5
70	23902.0
71	20277.5
72	16653.0
73	13252.5
74	9852.0
75	9852.0
76	7897.0
77	5942.0
78	4800.0
79	3658.0
80	2868.0
81	2078.0
82	2078.0
83	1698.5
84	1319.0
85	1044.5
86	770.0
87	588.0
88	406.0
89	406.0
90	315.5
91	225.0
92	161.0
93	97.0
94	64.0
95	31.0
96	31.0
97	24.0
98	17.0
99	10.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1128927.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.40571596815113
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.43222236255642	41.35407890209835
2	7.284525517872208	7.052253463617422
3	2.17646796880275	3.1606047093493426
4	1.124256787949401	2.176818190109785
5	0.7124675489288251	1.7243750904986765
6	0.4842871875559317	1.40653608287083
7	0.3658044036990766	1.2394916845749475
8	0.27204084925247285	1.0534665664519856
9	0.20713901297805584	0.9024041005325047
>10	1.5972191216031681	15.425050293337112
>50	0.20357708289037274	6.9079264230416895
>100	0.12621340057847055	12.23793085380306
>500	0.012125304692967957	4.214186514864796
>1k	0.001653450639950176	1.1448771248495269
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	2179	0.19301513738266513	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1872	0.1658211735568376	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1624	0.14385341124802578	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	1463	0.12959208168464392	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	1184	0.10487834908723061	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1179	0.10443545065358523	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	1169	0.10354965378629442	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.857968672907991E-5
2	0.0	0.0	0.0	0.0	8.857968672907991E-5
3	0.0	0.0	0.0	0.0	8.857968672907991E-5
4	0.0	0.0	0.0	0.0	8.857968672907991E-5
5	0.0	0.0	0.0	0.0	8.857968672907991E-5
6	0.0	0.0	0.0	0.0	8.857968672907991E-5
7	0.0	0.0	0.0	0.0	8.857968672907991E-5
8	0.0	0.0	0.0	0.0	8.857968672907991E-5
9	0.0	0.0	0.0	0.0	8.857968672907991E-5
10	0.0	0.0	0.0	0.0	8.857968672907991E-5
11	0.0	0.0	0.0	0.0	8.857968672907991E-5
12	0.0	0.0	0.0	0.0	8.857968672907991E-5
13	0.0	0.0	0.0	0.0	8.857968672907991E-5
14	0.0	0.0	0.0	1.7715937345815983E-4	1.7715937345815983E-4
15	0.0	0.0	0.0	4.428984336453996E-4	1.7715937345815983E-4
16	0.0	0.0	0.0	7.086374938326393E-4	1.7715937345815983E-4
17	0.0	0.0	0.0	0.0015058546743943585	1.7715937345815983E-4
18	0.0	0.0	0.0	0.001860173421310678	1.7715937345815983E-4
19	8.857968672907991E-5	0.0	0.0	0.0022144921682269976	1.7715937345815983E-4
20	8.857968672907991E-5	0.0	0.0	0.003188868722246877	1.7715937345815983E-4
21	8.857968672907991E-5	0.0	0.0	0.004606143709912155	1.7715937345815983E-4
22	8.857968672907991E-5	0.0	0.0	0.007174954625055473	2.6573906018723977E-4
23	8.857968672907991E-5	0.0	0.0	0.008769388986178912	2.6573906018723977E-4
24	8.857968672907991E-5	0.0	0.0	0.013021213949174748	2.6573906018723977E-4
25	8.857968672907991E-5	0.0	0.0	0.015412865490859904	2.6573906018723977E-4
26	8.857968672907991E-5	0.0	0.0	0.023385037296477097	2.6573906018723977E-4
27	8.857968672907991E-5	0.0	0.0	0.077595805574674	2.6573906018723977E-4
28	1.7715937345815983E-4	0.0	0.0	0.291515749025402	2.6573906018723977E-4
29	1.7715937345815983E-4	0.0	0.0	0.5834743964844494	2.6573906018723977E-4
30	1.7715937345815983E-4	0.0	0.0	0.9534717479518162	2.6573906018723977E-4
31	1.7715937345815983E-4	0.0	0.0	1.9869309530199915	2.6573906018723977E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1555	0.0	29.623795	1
TATATCT	75	3.7406426E-7	22.199999	5
TACCTTA	60	3.7262114E-5	21.583332	31
GACCGTT	115	1.3460522E-10	20.913042	7
AACCGTC	65	6.901993E-5	19.923077	16
CCCGTAT	65	6.901993E-5	19.923077	1
GTATCAA	2325	0.0	19.653763	2
ATACGGC	200	0.0	19.425	29
GTACTAT	105	2.2593667E-8	19.38095	1
TATACAC	740	0.0	19.25	37
CTTATAC	2405	0.0	19.076923	37
ATACACA	400	0.0	18.9625	37
CGTTATT	60	9.2366786E-4	18.5	10
ATTGTAC	50	0.007034808	18.499998	3
ATAGTAC	50	0.007034808	18.499998	3
GGACCGT	280	0.0	17.839285	6
GTTTATA	115	6.404662E-8	17.69565	1
GCGGTAA	1110	0.0	17.5	23
CGCGGTA	1165	0.0	17.309011	22
TTTTACG	75	2.0672257E-4	17.266666	4
>>END_MODULE