ERR1632714.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	ERR1632714.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	21474
Sequences flagged as poor quality	0
Sequence length	43
%GC	55

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	69	0.3213188041352333	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	66	0.3073484213467449	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	45	0.20955574182732606	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	44	0.2048989475644966	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	38	0.1769581819875198	No Hit
GATCAGCAGGCAGCAGCGATGGCTCCGAACCTCGCTCCTAAAG	36	0.16764459346186086	No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC	35	0.1629877991990314	No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC	35	0.1629877991990314	No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT	34	0.15833100493620192	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	33	0.15367421067337245	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	32	0.149017416410543	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	32	0.149017416410543	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	29	0.13504703362205459	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	29	0.13504703362205459	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	28	0.1303902393592251	No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG	28	0.1303902393592251	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	27	0.12573344509639564	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	27	0.12573344509639564	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	27	0.12573344509639564	No Hit
GGTTCAGACAGCAGCTCTGCCAAGAAGTACTTGGCCAGTTCCT	26	0.12107665083356618	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	26	0.12107665083356618	No Hit
GAGCTGGAGAGTGGCTGGTCAAACTCTAGGCGCGGATCAGCAG	26	0.12107665083356618	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	26	0.12107665083356618	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	26	0.12107665083356618	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	25	0.1164198565707367	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	25	0.1164198565707367	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	24	0.11176306230790724	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	24	0.11176306230790724	No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT	24	0.11176306230790724	TruSeq Adapter, Index 1 (95% over 21bp)
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	24	0.11176306230790724	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	23	0.10710626804507778	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	23	0.10710626804507778	No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA	23	0.10710626804507778	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	23	0.10710626804507778	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	23	0.10710626804507778	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	23	0.10710626804507778	No Hit
CTGCTGATCCGCGCCTAGAGTTTGACCAGCCACTCTCCAGCTC	22	0.1024494737822483	No Hit
GTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCA	22	0.1024494737822483	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTGCC	30	7.937959E-6	37.0	9
GCGGTAA	30	7.937959E-6	37.0	23
GCCAGCA	30	7.937959E-6	37.0	13
AACTCCG	30	7.937959E-6	37.0	5
CGCGGTA	30	7.937959E-6	37.0	22
CGTGCCA	30	7.937959E-6	37.0	10
TCCGTGC	30	7.937959E-6	37.0	8
AGCCGCG	30	7.937959E-6	37.0	19
CTCCGTG	30	7.937959E-6	37.0	7
CAGCCGC	30	7.937959E-6	37.0	18
CTAACTC	40	1.505432E-6	32.375	3
CCAGCAG	35	2.2816788E-5	31.714285	14
GCAGCCG	35	2.2816788E-5	31.714285	17
TAACTCC	35	2.2816788E-5	31.714285	4
GCTAACT	35	2.2816788E-5	31.714285	2
GGCTAAC	35	2.2816788E-5	31.714285	1
GTGCCAG	35	2.2816788E-5	31.714285	11
TGCCAGC	40	5.6770077E-5	27.75	12
GCCGCGG	40	5.6770077E-5	27.75	20
CCGCGGT	40	5.6770077E-5	27.75	21

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content