##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632714.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	21474
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.97592437366117	34.0	33.0	34.0	31.0	34.0
2	33.13276520443327	34.0	33.0	34.0	31.0	34.0
3	33.21397969637702	34.0	33.0	34.0	31.0	34.0
4	36.507637142591044	37.0	37.0	37.0	35.0	37.0
5	36.48356151625221	37.0	37.0	37.0	35.0	37.0
6	36.52039675887119	37.0	37.0	37.0	35.0	37.0
7	36.51289932010804	37.0	37.0	37.0	35.0	37.0
8	36.48672813635093	37.0	37.0	37.0	35.0	37.0
9	38.29673093042749	39.0	39.0	39.0	37.0	39.0
10	38.32206389121728	39.0	39.0	39.0	37.0	39.0
11	38.38604824438856	39.0	39.0	39.0	37.0	39.0
12	38.34218124243271	39.0	39.0	39.0	37.0	39.0
13	38.37608270466611	39.0	39.0	39.0	37.0	39.0
14	39.9140821458508	41.0	40.0	41.0	38.0	41.0
15	39.89042563099562	41.0	40.0	41.0	38.0	41.0
16	39.87966843624849	41.0	40.0	41.0	38.0	41.0
17	39.83752444816988	41.0	40.0	41.0	38.0	41.0
18	39.8629039769023	41.0	40.0	41.0	38.0	41.0
19	39.8579677749837	41.0	40.0	41.0	38.0	41.0
20	39.82443885629133	41.0	40.0	41.0	38.0	41.0
21	39.797569153394804	41.0	40.0	41.0	38.0	41.0
22	39.76934898016206	41.0	40.0	41.0	38.0	41.0
23	39.759336872496974	41.0	40.0	41.0	38.0	41.0
24	39.729533389214865	41.0	40.0	41.0	38.0	41.0
25	39.69521281549781	41.0	40.0	41.0	37.0	41.0
26	39.58042283691906	41.0	40.0	41.0	37.0	41.0
27	39.48044146409612	41.0	39.0	41.0	37.0	41.0
28	39.472524913849306	41.0	39.0	41.0	37.0	41.0
29	39.42008941044985	41.0	39.0	41.0	37.0	41.0
30	39.392428052528636	41.0	39.0	41.0	37.0	41.0
31	39.28071155816336	41.0	39.0	41.0	36.0	41.0
32	39.22124429542703	41.0	39.0	41.0	36.0	41.0
33	39.13816708577815	41.0	39.0	41.0	35.0	41.0
34	39.083496321132536	41.0	39.0	41.0	35.0	41.0
35	39.02086243829748	40.0	39.0	41.0	35.0	41.0
36	38.940812144919434	40.0	39.0	41.0	35.0	41.0
37	38.87380087547732	40.0	39.0	41.0	35.0	41.0
38	38.804135233305395	40.0	38.0	41.0	35.0	41.0
39	38.68608549874266	40.0	38.0	41.0	35.0	41.0
40	38.58927074601844	40.0	38.0	41.0	35.0	41.0
41	38.507637142591044	40.0	38.0	41.0	35.0	41.0
42	38.42605010710627	40.0	37.0	41.0	35.0	41.0
43	37.66880879202757	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	2.0
20	2.0
21	1.0
22	3.0
23	6.0
24	7.0
25	19.0
26	31.0
27	43.0
28	51.0
29	76.0
30	114.0
31	139.0
32	166.0
33	248.0
34	370.0
35	543.0
36	900.0
37	1815.0
38	4422.0
39	12514.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.952035019092854	15.41864580422837	12.014529198100028	27.614789978578745
2	21.55164384837478	19.237217099748534	31.638260221663405	27.57287883021328
3	21.663406910682685	18.762224084939927	28.57408959672162	31.000279407655768
4	15.558349632113252	15.018161497625035	33.78038558256497	35.643103287696746
5	17.714445375803297	32.001490174164104	32.92353543820434	17.36052901182826
6	35.880599795101055	32.84436993573624	14.915712023842786	16.35931824531992
7	31.493899599515697	26.241035671044056	18.73894011362578	23.526124615814474
8	28.415758591785416	29.43093974108224	20.14529198100028	22.008009686132066
9	28.10375337617584	11.725807953804601	16.326720685480115	43.843717984539445
10	19.52593834404396	22.645990500139703	28.90006519511968	28.928005960696655
11	41.729533389214865	18.268603893080005	17.77032690695725	22.231535810747882
12	22.981279687063424	22.93936853869796	25.440067057837386	28.63928471640123
13	36.79333147061563	16.5455900158331	19.54456552109528	27.116512992455995
14	25.73344509639564	18.594579491478065	22.026636863183384	33.64533854894291
15	29.905932755890845	22.636676911614046	19.60976064077489	27.847629691720222
16	27.801061749091925	22.347955667318615	21.751886001676446	28.099096581913013
17	27.717239452360992	22.701872031293657	20.517835522026637	29.06305299431871
18	26.77191021700661	19.931079444910125	23.363136816615445	29.933873521467824
19	30.073577349352703	21.70531805904815	21.942814566452455	26.27829002514669
20	31.47061562820155	19.71686690881997	20.978858154046755	27.83365930893173
21	27.996647108130762	21.2070410729254	20.48989475644966	30.30641706249418
22	29.500791655024685	21.579584613951756	20.187203129365745	28.73242060165782
23	28.29468194095185	21.04405327372637	20.871751886001675	29.78951289932011
24	28.741734190183475	20.68082332122567	21.374685666387258	29.202756822203597
25	29.505448449287506	21.039396479463537	22.05457762876036	27.40057744248859
26	29.118934525472667	22.096488777125828	20.033528918692372	28.751047778709136
27	28.620657539349914	21.12321877619447	22.035950451709045	28.220173232746575
28	26.71602868585266	22.306044518953154	21.295520163919157	29.682406631275033
29	28.136350936015646	23.04181801248021	21.360715283598772	27.461115767905376
30	28.4762969172022	20.74136164664245	21.723945236099468	29.05839620005588
31	27.996647108130762	21.2862065753935	21.896246623824158	28.82089969265158
32	26.674117537487195	21.309490546707647	21.919530595138305	30.09686132066685
33	27.149110552295802	20.84846791468753	23.088385955108503	28.91403557790817
34	27.367979882648786	21.16978671882276	23.10701313215982	28.35522026636863
35	27.05131787277638	21.90090341808699	22.524913849306138	28.522864859830495
36	26.62289280059607	21.165129924559935	23.40504796498091	28.80692930986309
37	27.018720312936573	20.74136164664245	24.36900437738661	27.87091366303437
38	26.08736146037068	20.13132159821179	24.583216913476765	29.198100027940765
39	25.076837105336686	19.945049827698615	26.818478159634907	28.159634907329796
40	25.73810189065847	19.861227530967682	27.121169786718824	27.279500791655025
41	23.10701313215982	19.935736239172954	27.819688926143243	29.137561702523985
42	21.155816336034274	20.33622054577629	29.929216727204995	28.578746390984445
43	21.78448356151625	20.49455155071249	28.611343950824253	29.109620936947007
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	2.5
4	5.0
5	5.0
6	3.5
7	2.0
8	1.5
9	1.0
10	2.5
11	4.0
12	4.0
13	6.0
14	8.0
15	12.0
16	16.0
17	20.0
18	24.0
19	24.0
20	25.5
21	27.0
22	22.5
23	18.0
24	24.5
25	31.0
26	31.0
27	28.0
28	25.0
29	41.0
30	57.0
31	72.0
32	87.0
33	87.0
34	128.0
35	169.0
36	209.0
37	249.0
38	315.0
39	381.0
40	381.0
41	441.0
42	501.0
43	629.0
44	757.0
45	1012.5
46	1268.0
47	1268.0
48	1425.0
49	1582.0
50	1828.5
51	2075.0
52	2283.0
53	2491.0
54	2491.0
55	2380.5
56	2270.0
57	2107.0
58	1944.0
59	1989.5
60	2035.0
61	2035.0
62	1828.0
63	1621.0
64	1334.5
65	1048.0
66	947.5
67	847.0
68	847.0
69	750.0
70	653.0
71	636.0
72	619.0
73	516.0
74	413.0
75	413.0
76	267.5
77	122.0
78	89.5
79	57.0
80	45.5
81	34.0
82	34.0
83	24.0
84	14.0
85	12.5
86	11.0
87	8.5
88	6.0
89	6.0
90	3.5
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	21474.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.45711092483934
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.49505991170906	56.81754680078234
2	6.635834909957255	8.819968333799013
3	2.732814799243221	5.448449287510478
4	1.2612991381122556	3.352891869237217
5	0.9459743535841917	3.143336127409891
6	0.6166351341882138	2.458787370773959
7	0.4694835680751174	2.1840365092670204
8	0.27328147992432206	1.452919810002794
9	0.27328147992432206	1.6345347862531434
>10	1.2823207904141265	14.058861879482166
>50	0.014014434867913952	0.6286672254819782
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	69	0.3213188041352333	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	66	0.3073484213467449	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	45	0.20955574182732606	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	44	0.2048989475644966	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	38	0.1769581819875198	No Hit
GATCAGCAGGCAGCAGCGATGGCTCCGAACCTCGCTCCTAAAG	36	0.16764459346186086	No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC	35	0.1629877991990314	No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC	35	0.1629877991990314	No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT	34	0.15833100493620192	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	33	0.15367421067337245	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	32	0.149017416410543	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	32	0.149017416410543	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	29	0.13504703362205459	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	29	0.13504703362205459	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	28	0.1303902393592251	No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG	28	0.1303902393592251	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	27	0.12573344509639564	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	27	0.12573344509639564	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	27	0.12573344509639564	No Hit
GGTTCAGACAGCAGCTCTGCCAAGAAGTACTTGGCCAGTTCCT	26	0.12107665083356618	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	26	0.12107665083356618	No Hit
GAGCTGGAGAGTGGCTGGTCAAACTCTAGGCGCGGATCAGCAG	26	0.12107665083356618	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	26	0.12107665083356618	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	26	0.12107665083356618	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	25	0.1164198565707367	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	25	0.1164198565707367	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	24	0.11176306230790724	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	24	0.11176306230790724	No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT	24	0.11176306230790724	TruSeq Adapter, Index 1 (95% over 21bp)
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	24	0.11176306230790724	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	23	0.10710626804507778	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	23	0.10710626804507778	No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA	23	0.10710626804507778	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	23	0.10710626804507778	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	23	0.10710626804507778	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	23	0.10710626804507778	No Hit
CTGCTGATCCGCGCCTAGAGTTTGACCAGCCACTCTCCAGCTC	22	0.1024494737822483	No Hit
GTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCA	22	0.1024494737822483	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.004656794262829468	0.0
17	0.0	0.0	0.0	0.004656794262829468	0.0
18	0.0	0.0	0.0	0.004656794262829468	0.0
19	0.0	0.0	0.0	0.004656794262829468	0.0
20	0.0	0.0	0.0	0.004656794262829468	0.0
21	0.0	0.0	0.0	0.004656794262829468	0.0
22	0.0	0.0	0.0	0.004656794262829468	0.0
23	0.0	0.0	0.0	0.009313588525658936	0.0
24	0.0	0.0	0.0	0.009313588525658936	0.0
25	0.0	0.0	0.0	0.009313588525658936	0.0
26	0.0	0.0	0.0	0.018627177051317872	0.0
27	0.0	0.0	0.0	0.041911148365465216	0.0
28	0.0	0.0	0.0	0.1909285647760082	0.0
29	0.0	0.0	0.0	0.3352891869237217	0.0
30	0.0	0.0	0.0	0.5634721058023656	0.0
31	0.0	0.0	0.0	1.2806184222781039	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTGCC	30	7.937959E-6	37.0	9
GCGGTAA	30	7.937959E-6	37.0	23
GCCAGCA	30	7.937959E-6	37.0	13
AACTCCG	30	7.937959E-6	37.0	5
CGCGGTA	30	7.937959E-6	37.0	22
CGTGCCA	30	7.937959E-6	37.0	10
TCCGTGC	30	7.937959E-6	37.0	8
AGCCGCG	30	7.937959E-6	37.0	19
CTCCGTG	30	7.937959E-6	37.0	7
CAGCCGC	30	7.937959E-6	37.0	18
CTAACTC	40	1.505432E-6	32.375	3
CCAGCAG	35	2.2816788E-5	31.714285	14
GCAGCCG	35	2.2816788E-5	31.714285	17
TAACTCC	35	2.2816788E-5	31.714285	4
GCTAACT	35	2.2816788E-5	31.714285	2
GGCTAAC	35	2.2816788E-5	31.714285	1
GTGCCAG	35	2.2816788E-5	31.714285	11
TGCCAGC	40	5.6770077E-5	27.75	12
GCCGCGG	40	5.6770077E-5	27.75	20
CCGCGGT	40	5.6770077E-5	27.75	21
>>END_MODULE