FastQCFastQC Report
Fri 10 Feb 2017
ERR1632713.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632713.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78737
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA3340.42419701030011303No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA2510.3187827831896059No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1910.2425797274470706No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA1850.2349594218728171No Hit
TCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT1830.2324193200147326Illumina PCR Primer Index 11 (95% over 22bp)
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT1780.22606906536952132No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT1670.21209850515005652No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC1620.20574825050484524No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA1580.20066804678867622No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT1520.1930477412144227No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC1470.18669748656921142No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA1400.17780713006591564No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC1380.17526702820783113No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC1340.17018682449166211No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT1300.1651066207754931No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA1290.16383656984645084No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT1250.15875636613028182No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA1220.15494621334315506No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA1210.1536761624141128No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA1210.1536761624141128No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG1180.14986600962698604No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA1160.14732590776890153No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA1120.1422457040527325No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC1120.1422457040527325No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC1100.139705602194648No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC1080.1371655003365635No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC1070.13589544940752124No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT1030.13081524569135222No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC980.12446499104614094No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC970.12319494011709871No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCGGGCTGCGTCT940.11938478732997193No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA890.11303453268476066No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA870.11049443082667616No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA860.1092243798976339No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC860.1092243798976339No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA850.10795432896859164No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC850.10795432896859164No Hit
CATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTC830.10541422711050712No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG810.10287412525242262No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG810.10287412525242262No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC800.10160407432338037No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA790.10033402339433811No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT790.10033402339433811No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGATG200.001831538937.030
GAGAGAT200.001831538937.029
CGAGAGA200.001831538937.026
ATACGGC250.005465672429.629
AGATGGA250.005465672429.632
CCGAGAG250.005465672429.625
ATCTAAG453.9462448E-628.77777932
CATCTAA453.9462448E-628.77777931
GGTATCA1200.027.7500021
AAGTACC358.7958865E-426.42857236
GCGGTTT358.7958865E-426.42857228
CTAAGTA358.7958865E-426.42857234
GAAACAT508.9514215E-625.90000227
AAACATC508.9514215E-625.90000228
ACATCTA508.9514215E-625.90000230
TCTAAGT508.9514215E-625.90000233
AACATCT508.9514215E-625.90000229
ACTGAAA601.3111585E-624.66666824
TTAGCCC451.3079758E-424.66666831
CAATGAG551.8736337E-523.54545433
Produced by FastQC (version 0.11.5)