FastQCFastQC Report
Fri 10 Feb 2017
ERR1632712.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632712.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55748
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA830.14888426490636436No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA760.1363277606371529No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT690.12377125636794145No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA690.12377125636794145No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA680.12197747004376838No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA660.11838989739542227No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG640.11480232474707613No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA640.11480232474707613No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC630.11300853842290307No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC630.11300853842290307No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC630.11300853842290307No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA620.11121475209873No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA620.11121475209873No Hit
GATCAGCAGGCAGCAGCGATGGCTCCGAACCTCGCTCCTAAAG590.1058333931262108No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT580.10403960680203773No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC580.10403960680203773No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA570.10224582047786467No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC560.10045203415369162No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA401.564189E-632.3751
TATACAC601.2150849E-930.83333437
GCCTGCT303.5560704E-430.83333420
TTCGGAG303.5560704E-430.8333342
GTTCGGA303.5560704E-430.8333341
CGAAGGT250.00545211329.5999984
GCTGCCT358.7634416E-426.42857217
TCGCTGC358.7634416E-426.42857212
ATCGCTG358.7634416E-426.42857211
AACCGGG400.001908336323.12514
ACTTCAT400.001908336323.12531
TGCCTGC400.001908336323.12519
CATCGCT400.001908336323.12510
TTATACA854.9612936E-821.76470636
GTATCAA603.6450354E-521.5833342
AGCCATC450.003780969220.5555557
CCTGCTG450.003780969220.55555521
CTGATTC450.003780969220.5555559
GGTTAAT450.003780969220.5555551
ATCGCAC555.0591596E-420.18181826
Produced by FastQC (version 0.11.5)