##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632712.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	55748
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.00048432230753	34.0	33.0	34.0	31.0	34.0
2	33.14343115448088	34.0	33.0	34.0	31.0	34.0
3	33.210160005740114	34.0	33.0	34.0	31.0	34.0
4	36.51212599555141	37.0	37.0	37.0	35.0	37.0
5	36.48143431154481	37.0	37.0	37.0	35.0	37.0
6	36.52446724546172	37.0	37.0	37.0	35.0	37.0
7	36.51980340101887	37.0	37.0	37.0	35.0	37.0
8	36.49949773982923	37.0	37.0	37.0	35.0	37.0
9	38.29931118605152	39.0	39.0	39.0	37.0	39.0
10	38.33030781373323	39.0	39.0	39.0	37.0	39.0
11	38.39874076200043	39.0	39.0	39.0	37.0	39.0
12	38.35622802611753	39.0	39.0	39.0	37.0	39.0
13	38.38094640166463	39.0	39.0	39.0	37.0	39.0
14	39.91660687378919	41.0	40.0	41.0	38.0	41.0
15	39.92021238430078	41.0	40.0	41.0	38.0	41.0
16	39.869394417736956	41.0	40.0	41.0	38.0	41.0
17	39.8719057185908	41.0	40.0	41.0	38.0	41.0
18	39.88510798593671	41.0	40.0	41.0	38.0	41.0
19	39.868120829446795	41.0	40.0	41.0	38.0	41.0
20	39.862075769534336	41.0	40.0	41.0	38.0	41.0
21	39.81665709980627	41.0	40.0	41.0	38.0	41.0
22	39.79192078639593	41.0	40.0	41.0	38.0	41.0
23	39.76374040324317	41.0	40.0	41.0	38.0	41.0
24	39.748905790342256	41.0	40.0	41.0	38.0	41.0
25	39.69328047642965	41.0	40.0	41.0	38.0	41.0
26	39.607429862954724	41.0	40.0	41.0	37.0	41.0
27	39.510547463586136	41.0	39.0	41.0	37.0	41.0
28	39.475927387529595	41.0	39.0	41.0	37.0	41.0
29	39.43775561455119	41.0	39.0	41.0	37.0	41.0
30	39.37617493004233	41.0	39.0	41.0	37.0	41.0
31	39.30419028485327	41.0	39.0	41.0	36.0	41.0
32	39.249354236923296	41.0	39.0	41.0	36.0	41.0
33	39.191612255148165	41.0	39.0	41.0	35.0	41.0
34	39.13381645978331	41.0	39.0	41.0	35.0	41.0
35	39.06995766664275	41.0	39.0	41.0	35.0	41.0
36	39.010798593671524	40.0	39.0	41.0	35.0	41.0
37	38.93694841070532	40.0	39.0	41.0	35.0	41.0
38	38.88256080935639	40.0	38.0	41.0	35.0	41.0
39	38.81461218339671	40.0	38.0	41.0	35.0	41.0
40	38.70386381574227	40.0	38.0	41.0	35.0	41.0
41	38.6590012197747	40.0	38.0	41.0	35.0	41.0
42	38.572845662624665	40.0	38.0	41.0	35.0	41.0
43	37.83782377843151	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	0.0
19	3.0
20	2.0
21	4.0
22	11.0
23	9.0
24	32.0
25	49.0
26	69.0
27	78.0
28	116.0
29	197.0
30	257.0
31	329.0
32	407.0
33	633.0
34	920.0
35	1441.0
36	2320.0
37	4737.0
38	11123.0
39	33009.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.044629403745425	15.754825285212027	12.382506995766663	27.818038315275885
2	21.56489918920858	18.56210088254287	32.275597330845955	27.5974025974026
3	21.568486761856928	18.251775848460934	28.497883332137476	31.68185405754467
4	15.33507928535553	15.632847815168258	33.364425629619	35.66764726985722
5	17.90019372892301	32.133888211236275	32.52672741623018	17.439190643610534
6	35.90622085097223	32.307885484681066	15.182607447800816	16.603286216545886
7	31.888139484824567	26.521130802898757	19.423118318145942	22.16761139413073
8	28.583985075697782	30.12843510081079	19.56662122407979	21.720958599411638
9	28.44765731506063	12.251560594102031	16.791633780584057	42.50914831025328
10	19.87694625816173	22.743416804190286	29.141852622515607	28.23778431513238
11	40.51983927674536	18.897538925163236	17.573724617923514	23.0088971801679
12	23.299490564683936	22.89409485542082	26.04218985434455	27.76422472555069
13	36.36901772260888	16.562029131089908	20.21059051445792	26.858362631843296
14	25.186553777714	19.103824352443137	22.409772547894093	33.29984932194877
15	29.51854775059195	23.685154624381145	19.75496878811796	27.041328836908946
16	27.240439118892155	22.562244385448803	21.920068881394847	28.27724761426419
17	27.267345913754752	22.854631556289014	21.428571428571427	28.449451101384803
18	27.79651287938581	20.389969146875224	22.190930616344982	29.622587357393986
19	30.29525722895889	21.911099949773984	21.615125206285427	26.178517614981704
20	31.271076989309034	20.51194661691899	21.645619573796367	26.571356819975605
21	28.38487479371457	21.453684437109853	20.96039319796226	29.20104757121332
22	29.667432015498314	21.77477218913683	20.169333429001938	28.388462366362923
23	27.88440840927029	21.328119394417737	21.527229676400946	29.260242519911028
24	28.16782664848963	21.005237856066586	21.72813374470833	29.098801750735454
25	29.09342039176293	21.116452608165314	22.146085958240654	27.6440410418311
26	28.5229963406759	22.040252565114443	21.267130659395853	28.1696204348138
27	28.322092272368515	21.202554351725624	22.684221855492574	27.791131520413288
28	27.40008610174356	22.51919351366865	21.374757838846236	28.705962545741553
29	28.35796799885198	22.960464949415226	21.68687665925235	26.994690392480447
30	28.300566836478442	21.261749300423332	22.404391188921576	28.033292674176653
31	27.583052306809215	21.213317069670662	22.416947693190785	28.786682930329338
32	25.859223649278896	21.64741336012054	22.897682428069167	29.595680562531392
33	26.668221281480953	20.42225730071034	23.8878524790127	29.021668938796008
34	27.001865537777142	20.77922077922078	24.368587213891082	27.850326469110996
35	26.56238788835474	21.105689890220276	24.39728779507785	27.93463442634713
36	25.694195307454976	20.728994762143934	24.906723111143002	28.67008681925809
37	25.340819401592885	20.08861304441415	26.406328478151682	28.164239075841284
38	24.564109923225946	18.996197172992755	26.686159144722684	29.75353375905862
39	24.235847025902274	18.481380497955083	29.204635143861662	28.078137332280978
40	24.106694410561815	18.94776494224008	29.714070459926813	27.231470187271295
41	22.277032359905288	18.62488340388893	30.567912750233194	28.530171485972588
42	19.815957523139843	19.900265480375978	32.14106335653297	28.14271363995121
43	19.693980053096073	19.618641027480805	30.892588074908517	29.7947908445146
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	1.0
4	1.0
5	1.0
6	2.0
7	3.0
8	2.5
9	2.0
10	3.5
11	5.0
12	5.0
13	13.0
14	21.0
15	44.0
16	67.0
17	65.5
18	64.0
19	64.0
20	66.5
21	69.0
22	62.0
23	55.0
24	65.0
25	75.0
26	75.0
27	76.5
28	78.0
29	115.5
30	153.0
31	200.0
32	247.0
33	247.0
34	398.5
35	550.0
36	621.5
37	693.0
38	863.5
39	1034.0
40	1034.0
41	1224.0
42	1414.0
43	1776.5
44	2139.0
45	2892.0
46	3645.0
47	3645.0
48	4176.5
49	4708.0
50	5238.5
51	5769.0
52	6092.0
53	6415.0
54	6415.0
55	6181.5
56	5948.0
57	5441.0
58	4934.0
59	4893.5
60	4853.0
61	4853.0
62	4470.5
63	4088.0
64	3242.0
65	2396.0
66	2191.0
67	1986.0
68	1986.0
69	1748.0
70	1510.0
71	1444.0
72	1378.0
73	1118.5
74	859.0
75	859.0
76	561.0
77	263.0
78	208.0
79	153.0
80	124.0
81	95.0
82	95.0
83	68.5
84	42.0
85	34.5
86	27.0
87	15.5
88	4.0
89	4.0
90	3.5
91	3.0
92	2.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	55748.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.72519193513668
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.45068314718843	55.955370596254575
2	6.3437075631183655	8.211953792064289
3	2.447135770307347	4.751739972734448
4	1.2831527312030597	3.3220922723685153
5	0.6983898234625724	2.260170768458061
6	0.5099354266552116	1.9803401018870632
7	0.33810935897791206	1.5318935208437972
8	0.27713881883435415	1.4350290593384516
9	0.19953994956073495	1.1623735380641458
>10	1.3884654823601141	16.827509507067518
>50	0.06374192833190145	2.561526870919136
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	83	0.14888426490636436	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	76	0.1363277606371529	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	69	0.12377125636794145	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	69	0.12377125636794145	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	68	0.12197747004376838	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	66	0.11838989739542227	No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG	64	0.11480232474707613	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	64	0.11480232474707613	No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC	63	0.11300853842290307	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	63	0.11300853842290307	No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC	63	0.11300853842290307	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	62	0.11121475209873	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	62	0.11121475209873	No Hit
GATCAGCAGGCAGCAGCGATGGCTCCGAACCTCGCTCCTAAAG	59	0.1058333931262108	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	58	0.10403960680203773	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	58	0.10403960680203773	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	57	0.10224582047786467	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	56	0.10045203415369162	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0017937863241730646	0.0
21	0.0	0.0	0.0	0.0017937863241730646	0.0
22	0.0	0.0	0.0	0.003587572648346129	0.0
23	0.0	0.0	0.0	0.005381358972519194	0.0
24	0.0	0.0	0.0	0.007175145296692258	0.0
25	0.0	0.0	0.0	0.010762717945038388	0.0
26	0.0	0.0	0.0	0.014350290593384517	0.0
27	0.0	0.0	0.0	0.08430795723613403	0.0
28	0.0	0.0	0.0	0.28341823921934417	0.0
29	0.0	0.0	0.0	0.6547320083231686	0.0
30	0.0	0.0	0.0	1.0637152902346272	0.0
31	0.0	0.0	0.0	2.1686876659252348	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	40	1.564189E-6	32.375	1
TATACAC	60	1.2150849E-9	30.833334	37
GCCTGCT	30	3.5560704E-4	30.833334	20
TTCGGAG	30	3.5560704E-4	30.833334	2
GTTCGGA	30	3.5560704E-4	30.833334	1
CGAAGGT	25	0.005452113	29.599998	4
GCTGCCT	35	8.7634416E-4	26.428572	17
TCGCTGC	35	8.7634416E-4	26.428572	12
ATCGCTG	35	8.7634416E-4	26.428572	11
AACCGGG	40	0.0019083363	23.125	14
ACTTCAT	40	0.0019083363	23.125	31
TGCCTGC	40	0.0019083363	23.125	19
CATCGCT	40	0.0019083363	23.125	10
TTATACA	85	4.9612936E-8	21.764706	36
GTATCAA	60	3.6450354E-5	21.583334	2
AGCCATC	45	0.0037809692	20.555555	7
CCTGCTG	45	0.0037809692	20.555555	21
CTGATTC	45	0.0037809692	20.555555	9
GGTTAAT	45	0.0037809692	20.555555	1
ATCGCAC	55	5.0591596E-4	20.181818	26
>>END_MODULE