FastQCFastQC Report
Fri 10 Feb 2017
ERR1632711.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632711.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences375618
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA9680.2577086295118977No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA7290.19408015590307173No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA5940.1581393862913918No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT5730.15254859990735267No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT5640.15015254859990734No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA5030.13391264529388902No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC4840.12885431475594886No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA4510.12006879329531597No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC4150.11048458806553467No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT3940.10489380168149556No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA3920.1043613458353966No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC3900.10382888998929764No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGCG251.232044E-436.9999967
GGTATCA4350.029.7701171
CTCGTAA250.005491692629.59999831
TGGTACC250.005491692629.5999984
TAACCCT405.92965E-527.7535
CAGTCGG556.242899E-726.9090910
CACATAG358.8582345E-426.4285729
GCAGTCG509.06983E-625.8999989
ATACGGC659.3566996E-825.61538529
GCCGTCT451.3208964E-424.66666636
TACGGCT701.9157596E-723.78571530
AGTCGGT701.9157596E-723.78571511
CGGACCG400.001928780723.12517
GTATCAA5900.021.9491522
CTTATTG603.717695E-521.58333428
GCGGTAA3400.021.22058723
CTTATAC4150.020.95180737
TTAATGC450.00382108620.55555533
ATTACGC450.00382108620.5555553
CCGGACC450.00382108620.55555516
Produced by FastQC (version 0.11.5)