FastQCFastQC Report
Fri 10 Feb 2017
ERR1632710.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632710.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11274
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT840.7450771687067589Illumina PCR Primer Index 10 (95% over 22bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA660.5854177754124534No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA510.45236828100053217No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA430.38140855064750756No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC350.3104488202944829No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC340.30157885400035483No Hit
CATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTC330.2927088877062267No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT330.2927088877062267No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA320.28383892141209865No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA320.28383892141209865No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT300.2660989888238425No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA300.2660989888238425No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC280.24835905623558632No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC270.2394890899414582No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT270.2394890899414582No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA260.23061912364733014No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC260.23061912364733014No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA260.23061912364733014No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA230.2040092247649459No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC220.1951392584708178No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC210.18626929217668972No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA210.18626929217668972No Hit
CTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTC210.18626929217668972Illumina PCR Primer Index 10 (95% over 23bp)
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC200.17739932588256166No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA200.17739932588256166No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG200.17739932588256166No Hit
ACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCC190.16852935958843357No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC190.16852935958843357No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG190.16852935958843357No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG180.15965939329430548No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA180.15965939329430548No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA180.15965939329430548No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT180.15965939329430548No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA170.15078942700017742No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC170.15078942700017742No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA170.15078942700017742No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC170.15078942700017742No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT170.15078942700017742No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC170.15078942700017742No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA160.14191946070604933No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC160.14191946070604933No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC150.13304949441192124No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA150.13304949441192124No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC150.13304949441192124No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC150.13304949441192124No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT150.13304949441192124No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA150.13304949441192124No Hit
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGG140.12417952811779316No Hit
GCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTAC140.12417952811779316No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA140.12417952811779316No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC140.12417952811779316No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT140.12417952811779316No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA130.11530956182366507No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG130.11530956182366507No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGCTGTC130.11530956182366507No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT130.11530956182366507No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC130.11530956182366507No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA130.11530956182366507No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG130.11530956182366507No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA120.10643959552953698No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG120.10643959552953698No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA120.10643959552953698No Hit
CCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTC120.10643959552953698No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG120.10643959552953698No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT120.10643959552953698No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)