##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632710.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11274
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.9099698421146	34.0	33.0	34.0	31.0	34.0
2	33.07699130743303	34.0	33.0	34.0	31.0	34.0
3	33.138903672166045	34.0	33.0	34.0	31.0	34.0
4	36.44651410324641	37.0	37.0	37.0	35.0	37.0
5	36.43125776122051	37.0	37.0	37.0	35.0	37.0
6	36.47578499201703	37.0	37.0	37.0	35.0	37.0
7	36.484921057299985	37.0	37.0	37.0	35.0	37.0
8	36.431789959198156	37.0	37.0	37.0	35.0	37.0
9	38.2410856838744	39.0	39.0	39.0	37.0	39.0
10	38.2741706581515	39.0	39.0	39.0	37.0	39.0
11	38.329696647152744	39.0	39.0	39.0	37.0	39.0
12	38.28525811601916	39.0	39.0	39.0	37.0	39.0
13	38.344952989178644	39.0	39.0	39.0	37.0	39.0
14	39.805659038495655	41.0	40.0	41.0	38.0	41.0
15	39.825971261309206	41.0	40.0	41.0	38.0	41.0
16	39.783572822423274	41.0	40.0	41.0	38.0	41.0
17	39.784459819052685	41.0	40.0	41.0	38.0	41.0
18	39.815416001419194	41.0	40.0	41.0	38.0	41.0
19	39.788540003547986	41.0	40.0	41.0	38.0	41.0
20	39.79164449175093	41.0	40.0	41.0	38.0	41.0
21	39.73576370409793	41.0	40.0	41.0	38.0	41.0
22	39.70054993791024	41.0	40.0	41.0	38.0	41.0
23	39.697090651055525	41.0	40.0	41.0	38.0	41.0
24	39.69744544970729	41.0	40.0	41.0	38.0	41.0
25	39.638992371828984	41.0	40.0	41.0	37.0	41.0
26	39.52119921944296	41.0	40.0	41.0	37.0	41.0
27	39.37848146177045	41.0	39.0	41.0	36.0	41.0
28	39.362959020755724	41.0	39.0	41.0	36.0	41.0
29	39.29359588433564	41.0	39.0	41.0	36.0	41.0
30	39.267784282419726	41.0	39.0	41.0	36.0	41.0
31	39.19070427532375	41.0	39.0	41.0	36.0	41.0
32	39.11726095440837	41.0	39.0	41.0	35.0	41.0
33	38.98527585595175	40.0	39.0	41.0	35.0	41.0
34	38.980308674827036	40.0	39.0	41.0	35.0	41.0
35	38.881497250310446	40.0	39.0	41.0	35.0	41.0
36	38.83838921412099	40.0	39.0	41.0	35.0	41.0
37	38.751197445449705	40.0	38.0	41.0	35.0	41.0
38	38.70631541600142	40.0	38.0	41.0	35.0	41.0
39	38.57078233102714	40.0	38.0	41.0	35.0	41.0
40	38.47729288628703	40.0	38.0	41.0	35.0	41.0
41	38.39657619301047	40.0	38.0	41.0	35.0	41.0
42	38.327390455916266	40.0	37.0	41.0	35.0	41.0
43	37.56829874046478	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	3.0
22	3.0
23	4.0
24	8.0
25	7.0
26	13.0
27	26.0
28	37.0
29	34.0
30	62.0
31	90.0
32	125.0
33	128.0
34	193.0
35	327.0
36	514.0
37	933.0
38	2429.0
39	6338.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.20329962746142	15.824019868724498	13.340429306368637	28.632251197445452
2	22.529714387085328	19.717935071846725	29.838566613446872	27.91378392762108
3	21.678197622849034	18.662409082845485	28.933830051445803	30.72556324285968
4	15.247472059606174	15.868369700195139	34.72591804151144	34.158240198687245
5	17.25208444207912	32.04718822068477	32.63260599609721	18.068121341138905
6	32.68582579386198	33.46638282774526	16.09898882384247	17.74880255455029
7	29.28862870321093	26.64537874756076	19.194607060493173	24.871385488735143
8	27.381585949973392	27.949263792797584	21.27904913961327	23.390101117615753
9	28.091183253503637	12.045414227425935	16.569097037431256	43.294305481639164
10	20.276742948376796	21.491928330672344	27.79847436579741	30.43285435515345
11	40.34947667198865	19.10590739755189	17.52705339719709	23.017562533262375
12	22.370054993791026	23.620720241263083	26.441369522795817	27.56785524215008
13	38.05215540180947	16.169948554195493	18.51161965584531	27.266276388149723
14	24.56093666844066	19.842114599964518	22.600674117438356	32.99627461415647
15	30.956182366507008	23.39897108390988	17.589143161255986	28.055703388327125
16	25.643072556324288	23.487670746851162	22.024126308320028	28.845130388504526
17	28.268582579386198	23.842469398616284	20.001773993258826	27.88717402873869
18	27.017917331914138	19.407486251552246	22.547454319673584	31.027142096860032
19	28.75643072556324	21.64271775767252	22.032996274614156	27.56785524215008
20	31.94961859144935	19.292176689728578	20.835550824906864	27.922653893915207
21	28.570161433386552	20.729111229377327	20.57832180237715	30.122405534858963
22	29.492637927975874	22.529714387085328	18.946248004257583	29.031399680681215
23	31.151321624977825	20.25900301578854	20.879900656377508	27.709774702856127
24	28.800780557033885	19.247826858257937	21.802377150966826	30.14901543374135
25	30.0070959730353	21.385488735142808	21.31452900478978	27.292886287032108
26	29.439418130211102	22.10395600496718	21.731417420613802	26.725208444207908
27	27.73638460173851	20.800070959730352	24.23274791555792	27.23079652297321
28	26.388149725031045	23.17722192655668	20.746851161965584	29.68777718644669
29	28.41937200638637	23.461060847968778	20.285612914670924	27.833954230973923
30	26.760688309384424	20.649281532730175	24.126308320028382	28.463721837857015
31	28.72095086038673	21.704807521731418	21.438708532907576	28.13553308497428
32	26.689728579031403	20.57832180237715	22.095086038673053	30.636863579918394
33	26.476849387972322	20.791200993436227	24.126308320028382	28.605641298563068
34	26.92921766897286	21.633847791378393	22.893383005144578	28.543551534504168
35	26.41475962391343	23.36349121873337	23.806989533439772	26.41475962391343
36	28.162142983856665	21.46531843178996	23.709419904204367	26.66311868014902
37	28.08231328720951	21.341138903672167	23.13287209508604	27.443675714032285
38	26.609898882384247	20.507362072024126	24.534326769558277	28.34841227603335
39	26.54780911832535	19.318786588610966	25.669682455206672	28.463721837857015
40	26.1841405002661	20.276742948376796	27.328366152208623	26.210750399148488
41	23.922299095263437	19.638105375199576	27.39045591626752	29.04913961326947
42	21.624977825084265	20.498492105729998	30.415114422565193	27.46141564662054
43	21.252439240730887	22.095086038673053	27.34610608479688	29.306368635799185
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.0
4	2.0
5	2.0
6	2.0
7	2.0
8	1.5
9	1.0
10	1.0
11	1.0
12	1.0
13	3.5
14	6.0
15	10.0
16	14.0
17	11.5
18	9.0
19	9.0
20	13.0
21	17.0
22	12.0
23	7.0
24	11.5
25	16.0
26	16.0
27	18.0
28	20.0
29	23.0
30	26.0
31	28.5
32	31.0
33	31.0
34	41.5
35	52.0
36	53.5
37	55.0
38	87.5
39	120.0
40	120.0
41	181.5
42	243.0
43	292.5
44	342.0
45	543.0
46	744.0
47	744.0
48	855.5
49	967.0
50	1096.0
51	1225.0
52	1335.0
53	1445.0
54	1445.0
55	1288.5
56	1132.0
57	1113.5
58	1095.0
59	1083.0
60	1071.0
61	1071.0
62	1017.0
63	963.0
64	755.0
65	547.0
66	472.5
67	398.0
68	398.0
69	343.0
70	288.0
71	245.5
72	203.0
73	160.0
74	117.0
75	117.0
76	84.5
77	52.0
78	41.5
79	31.0
80	22.5
81	14.0
82	14.0
83	11.0
84	8.0
85	6.0
86	4.0
87	3.5
88	3.0
89	3.0
90	2.5
91	2.0
92	1.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	11274.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.61575306013837
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.97664961301325	58.133759091715454
2	6.834579561852289	9.242504878481462
3	2.4531024531024532	4.976051091005854
4	1.4823560278105732	4.009224764945893
5	0.7870916961826053	2.6609898882384244
6	0.4853732126459399	1.9691325172964342
7	0.3673094582185491	1.7385133936491042
8	0.3148366784730421	1.7030335284725917
9	0.18365472910927455	1.1176157530601383
>10	1.0756919847828939	12.666311868014901
>50	0.03935458480913026	1.7828632251197445
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT	84	0.7450771687067589	Illumina PCR Primer Index 10 (95% over 22bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	66	0.5854177754124534	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	51	0.45236828100053217	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	43	0.38140855064750756	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	35	0.3104488202944829	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	34	0.30157885400035483	No Hit
CATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTC	33	0.2927088877062267	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	33	0.2927088877062267	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	32	0.28383892141209865	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	32	0.28383892141209865	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	30	0.2660989888238425	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	30	0.2660989888238425	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	28	0.24835905623558632	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	27	0.2394890899414582	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	27	0.2394890899414582	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	26	0.23061912364733014	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	26	0.23061912364733014	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	26	0.23061912364733014	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	23	0.2040092247649459	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	22	0.1951392584708178	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	21	0.18626929217668972	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	21	0.18626929217668972	No Hit
CTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTC	21	0.18626929217668972	Illumina PCR Primer Index 10 (95% over 23bp)
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	20	0.17739932588256166	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	20	0.17739932588256166	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	20	0.17739932588256166	No Hit
ACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCC	19	0.16852935958843357	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	19	0.16852935958843357	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	19	0.16852935958843357	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	18	0.15965939329430548	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	18	0.15965939329430548	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	18	0.15965939329430548	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	18	0.15965939329430548	No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA	17	0.15078942700017742	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	17	0.15078942700017742	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	17	0.15078942700017742	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	17	0.15078942700017742	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	17	0.15078942700017742	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	17	0.15078942700017742	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	16	0.14191946070604933	No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC	16	0.14191946070604933	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	15	0.13304949441192124	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	15	0.13304949441192124	No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC	15	0.13304949441192124	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	15	0.13304949441192124	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	15	0.13304949441192124	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	15	0.13304949441192124	No Hit
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGG	14	0.12417952811779316	No Hit
GCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTAC	14	0.12417952811779316	No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA	14	0.12417952811779316	No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC	14	0.12417952811779316	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	14	0.12417952811779316	No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA	13	0.11530956182366507	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	13	0.11530956182366507	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGCTGTC	13	0.11530956182366507	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	13	0.11530956182366507	No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC	13	0.11530956182366507	No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA	13	0.11530956182366507	No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG	13	0.11530956182366507	No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	12	0.10643959552953698	No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG	12	0.10643959552953698	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	12	0.10643959552953698	No Hit
CCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTC	12	0.10643959552953698	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	12	0.10643959552953698	No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT	12	0.10643959552953698	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.008869966294128083	0.0
26	0.0	0.0	0.0	0.008869966294128083	0.0
27	0.0	0.0	0.0	0.026609898882384245	0.0
28	0.0	0.0	0.0	0.11530956182366507	0.0
29	0.0	0.0	0.0	0.23948908994145823	0.0
30	0.0	0.0	0.0	0.4434983147064041	0.0
31	0.0	0.0	0.0	0.9756962923540891	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE