FastQCFastQC Report
Fri 10 Feb 2017
ERR1632709.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632709.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences182274
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA5580.3061325257579249No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA4010.21999846385112523No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA3470.1903727355519712No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT3100.17007362542106938No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT3100.17007362542106938No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA2870.1574552596640223No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA2820.1547121366733599No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT2580.14154514631818033No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC2480.1360589003368555No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT2410.13221852814992813No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA2360.12947540515926573No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA2190.12014878699101353No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC2150.1179542885984836No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA2090.1146625410096887No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC2070.11356529181342374No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA2030.11137079342089382No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC2020.11082216882276133No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA1980.10862767043023142No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC1920.10533592284143652No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC1910.10478729824330403No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC1900.10423867364517156No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA1890.10369004904703907No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG1880.1031414244489066No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC1860.10204417525264163No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT1850.10149555065450913No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT1830.10039830145824417No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2700.030.148151
GCGGTAA2850.021.42105323
ACTGAAA909.368887E-820.55555524
ATACGGC450.003813653520.55555529
CGTGCCA3100.020.29032110
GGTTAAT1101.7262209E-920.1818181
GTATCAA4050.020.0987642
CGCGGTA3150.019.96825422
ATCTAAG759.195819E-619.73333432
CATCTAA759.195819E-619.73333431
ATGAGGC951.656881E-719.4736846
TATACAC951.656881E-719.47368437
TAATGAG951.656881E-719.4736844
AACTCCG3150.019.3809535
GGTAATA3200.019.07812525
CCGTGCC3200.019.0781259
AGCCGCG3300.019.06060419
TCCGTGC3300.019.0606048
AACGTCA801.6045427E-518.528
TACTTAG801.6045427E-518.52
Produced by FastQC (version 0.11.5)