##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632709.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	182274
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.64141347641463	34.0	31.0	34.0	31.0	34.0
2	32.808689116385224	34.0	31.0	34.0	31.0	34.0
3	32.90391388788308	34.0	31.0	34.0	31.0	34.0
4	36.35359952598835	37.0	37.0	37.0	35.0	37.0
5	36.222176503505715	37.0	37.0	37.0	35.0	37.0
6	36.22786025434236	37.0	37.0	37.0	35.0	37.0
7	36.240599317511	37.0	37.0	37.0	35.0	37.0
8	36.20201454952434	37.0	36.0	37.0	35.0	37.0
9	37.915407573213955	39.0	38.0	39.0	35.0	39.0
10	37.88864017907107	39.0	38.0	39.0	35.0	39.0
11	37.98350834458014	39.0	38.0	39.0	35.0	39.0
12	37.91692726335078	39.0	38.0	39.0	35.0	39.0
13	37.97321066087319	39.0	38.0	39.0	35.0	39.0
14	39.36486827523399	40.0	39.0	41.0	37.0	41.0
15	39.33859464322942	40.0	39.0	41.0	37.0	41.0
16	39.30902926363606	40.0	39.0	41.0	36.0	41.0
17	39.29503933638369	40.0	39.0	41.0	36.0	41.0
18	39.305792378507086	40.0	39.0	41.0	36.0	41.0
19	39.30106872071716	40.0	39.0	41.0	36.0	41.0
20	39.27107541393726	40.0	39.0	41.0	36.0	41.0
21	39.242695063475864	40.0	39.0	41.0	36.0	41.0
22	39.18795330107421	40.0	39.0	41.0	36.0	41.0
23	39.13312375873684	40.0	39.0	41.0	36.0	41.0
24	39.10870447787397	40.0	39.0	41.0	36.0	41.0
25	39.05994272359195	40.0	39.0	41.0	36.0	41.0
26	38.95736638247912	40.0	38.0	41.0	35.0	41.0
27	38.840372186927375	40.0	38.0	41.0	35.0	41.0
28	38.801326574278285	40.0	38.0	41.0	35.0	41.0
29	38.74119731832297	40.0	38.0	41.0	35.0	41.0
30	38.69802056244994	40.0	38.0	41.0	35.0	41.0
31	38.61836575704708	40.0	38.0	41.0	35.0	41.0
32	38.551526822256605	40.0	38.0	41.0	35.0	41.0
33	38.48670682598725	40.0	38.0	41.0	35.0	41.0
34	38.44524726510638	40.0	38.0	41.0	35.0	41.0
35	38.35044493454909	40.0	38.0	41.0	34.0	41.0
36	38.303065714254366	40.0	38.0	41.0	34.0	41.0
37	38.24008909663473	40.0	38.0	41.0	34.0	41.0
38	38.16495495792049	40.0	38.0	41.0	34.0	41.0
39	38.0479717348607	40.0	37.0	41.0	34.0	41.0
40	37.97487299340553	40.0	37.0	41.0	34.0	41.0
41	37.903409153252795	40.0	37.0	41.0	34.0	41.0
42	37.845474395690005	40.0	37.0	41.0	33.0	41.0
43	37.03302171456159	39.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	2.0
17	0.0
18	2.0
19	7.0
20	9.0
21	25.0
22	62.0
23	91.0
24	148.0
25	236.0
26	333.0
27	427.0
28	655.0
29	899.0
30	1180.0
31	1638.0
32	2232.0
33	3051.0
34	4703.0
35	7045.0
36	12134.0
37	24312.0
38	56218.0
39	66865.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.32598176371836	16.253552344272908	12.317719477270483	28.10274641473825
2	21.994360139131196	19.102559882374887	31.130056947233285	27.773023031260628
3	21.743638697784654	18.963209234449234	28.11097578371024	31.182176284055874
4	16.069763103898527	15.489866903672493	33.1199183646598	35.32045162776918
5	17.83468843609072	32.311794331610656	32.29698146746108	17.55653576483755
6	35.17451748466594	32.81707758649067	15.054258972755303	16.954145956088087
7	30.980831056541252	26.95118338325817	18.867748554374185	23.200237005826395
8	27.544246573839388	30.252257590221316	19.661059723273752	22.54243611266555
9	28.215763081953543	12.368192940298671	16.750606230180935	42.66543774756685
10	19.922205031984813	22.54298473726368	28.85875111096481	28.676059119786697
11	40.64265885425239	18.905055027047194	18.14136958644678	22.310916532253643
12	23.240286601490066	22.69166200335758	26.342210079331117	27.72584131582124
13	36.27176668093091	17.1604288049859	19.745547911386154	26.82225660269704
14	24.487858937643328	18.870491677364846	22.488670902048565	34.15297848294326
15	30.052558236501092	23.227668235733017	19.121213118711392	27.5985604090545
16	26.166650207928722	23.27539857577054	21.750222192962244	28.807729023338492
17	27.596914535260105	22.83924202025522	21.248779310269157	28.315064134215522
18	27.698958710512745	20.643646378529027	22.26647793980491	29.39091697115332
19	29.16159188913394	21.82264063991573	22.172663133524253	26.84310433742607
20	30.540834128839002	20.13836312364901	21.534612725896178	27.78619002161581
21	28.36169722505678	21.3914217057836	21.21421596058681	29.032665108572807
22	28.8872795900677	22.155655770982147	20.66120236566927	28.295862273280886
23	29.080944073208464	21.21860495737187	21.55052283924202	28.149928130177642
24	28.51256898954321	20.627736265183184	21.8204461415232	29.0392486037504
25	29.13470928382545	21.280050912362707	21.84897462062609	27.736265183185754
26	29.011268749245637	21.772715801485674	21.680546868999418	27.535468580269264
27	27.81197537772804	21.338753744362883	23.241932475284464	27.60733840262462
28	27.39008306176416	22.70153724612397	21.043593710567606	28.864785981544266
29	27.82020474670002	23.209563623994644	21.29870414869921	27.671527480606123
30	27.95132602565369	21.452319036176306	22.926473331358284	27.669881606811725
31	28.674961870590433	21.748027694569714	21.479201641484796	28.09780879335506
32	26.42944139043418	21.46438877733522	22.525428750123442	29.58074108210716
33	26.72515004882759	21.24164719049343	23.661630292855808	28.37157246782317
34	27.059262429090268	21.267981171203793	23.26552333300416	28.40723306670178
35	26.5259993197055	22.24453295587961	23.775744209267366	27.453723515147527
36	26.874375939519624	20.506490228995908	24.93005036373811	27.68908346774636
37	26.980809111557324	20.24479629568671	24.62940408396151	28.144990508794454
38	25.86545530355399	19.685199205591584	26.166650207928722	28.282695282925708
39	25.19064704785104	19.04111392738405	26.991781603519975	28.776457421244938
40	24.85324291999956	19.72250567826459	28.377607338402626	27.046644063333225
41	23.362629886873606	19.389490547198175	29.00249075567552	28.2453888102527
42	21.772167176887542	19.986942734564447	30.143081295192953	28.09780879335506
43	20.837310861669796	20.61072890264108	29.62408242535962	28.927877810329505
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	1.5
2	2.0
3	6.5
4	11.0
5	11.0
6	9.0
7	7.0
8	6.5
9	6.0
10	10.0
11	14.0
12	14.0
13	35.0
14	56.0
15	104.5
16	153.0
17	149.5
18	146.0
19	146.0
20	173.5
21	201.0
22	183.5
23	166.0
24	191.5
25	217.0
26	217.0
27	276.0
28	335.0
29	453.0
30	571.0
31	738.0
32	905.0
33	905.0
34	1177.5
35	1450.0
36	1703.5
37	1957.0
38	2579.5
39	3202.0
40	3202.0
41	3979.0
42	4756.0
43	5870.0
44	6984.0
45	9481.5
46	11979.0
47	11979.0
48	13870.0
49	15761.0
50	17315.0
51	18869.0
52	19511.5
53	20154.0
54	20154.0
55	19330.5
56	18507.0
57	17823.5
58	17140.0
59	16577.0
60	16014.0
61	16014.0
62	14746.0
63	13478.0
64	11324.5
65	9171.0
66	8101.0
67	7031.0
68	7031.0
69	5965.0
70	4899.0
71	4095.0
72	3291.0
73	2625.5
74	1960.0
75	1960.0
76	1634.0
77	1308.0
78	1020.5
79	733.0
80	576.0
81	419.0
82	419.0
83	315.5
84	212.0
85	163.0
86	114.0
87	90.5
88	67.0
89	67.0
90	43.5
91	20.0
92	11.5
93	3.0
94	3.0
95	3.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	182274.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.232639730957736
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.26756590837101	54.048560631958495
2	6.029340413607369	7.383848587234477
3	1.7366384015971161	3.190168607638275
4	0.994995442640396	2.4370478989257682
5	0.5580268908646313	1.7084729784250223
6	0.3613241460582605	1.327489875700885
7	0.27048814478363925	1.1593892184722203
8	0.2106006785496956	1.0316508381383003
9	0.190638405248285	1.0505963508707301
>10	1.185750762013619	13.80932017404568
>50	0.12975387084398973	5.703411181290628
>100	0.06387882872319495	6.809012427213769
>500	9.98106698799921E-4	0.3410312300857448
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	558	0.3061325257579249	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	401	0.21999846385112523	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	347	0.1903727355519712	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	310	0.17007362542106938	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	310	0.17007362542106938	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	287	0.1574552596640223	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	282	0.1547121366733599	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	258	0.14154514631818033	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	248	0.1360589003368555	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	241	0.13221852814992813	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	236	0.12947540515926573	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	219	0.12014878699101353	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	215	0.1179542885984836	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	209	0.1146625410096887	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	207	0.11356529181342374	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	203	0.11137079342089382	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	202	0.11082216882276133	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	198	0.10862767043023142	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	192	0.10533592284143652	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	191	0.10478729824330403	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	190	0.10423867364517156	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	189	0.10369004904703907	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	188	0.1031414244489066	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	186	0.10204417525264163	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	185	0.10149555065450913	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	183	0.10039830145824417	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	5.486245981324819E-4	0.0	0.0	0.0	0.0
9	5.486245981324819E-4	0.0	0.0	0.0	0.0
10	5.486245981324819E-4	0.0	0.0	0.0	0.0
11	5.486245981324819E-4	0.0	0.0	0.0	0.0
12	5.486245981324819E-4	0.0	0.0	0.0	0.0
13	5.486245981324819E-4	0.0	0.0	0.0	0.0
14	5.486245981324819E-4	0.0	0.0	0.0	0.0
15	5.486245981324819E-4	0.0	0.0	5.486245981324819E-4	0.0
16	5.486245981324819E-4	0.0	0.0	0.0010972491962649637	0.0
17	5.486245981324819E-4	0.0	0.0	0.0010972491962649637	0.0
18	5.486245981324819E-4	0.0	0.0	0.0010972491962649637	0.0
19	0.0010972491962649637	0.0	0.0	0.0016458737943974456	0.0
20	0.0010972491962649637	0.0	0.0	0.0027431229906624095	0.0
21	0.0010972491962649637	0.0	0.0	0.006583495177589782	0.0
22	0.0010972491962649637	0.0	0.0	0.010972491962649638	0.0
23	0.0010972491962649637	0.0	0.0	0.01152111656078212	0.0
24	0.0010972491962649637	0.0	0.0	0.016458737943974455	0.0
25	0.0010972491962649637	0.0	0.0	0.0181046117383719	0.0
26	0.0010972491962649637	0.0	0.0	0.022493608523431758	0.0
27	0.0010972491962649637	0.0	0.0	0.053216586018850744	0.0
28	0.0010972491962649637	0.0	0.0	0.19146998474823618	0.0
29	0.0010972491962649637	0.0	0.0	0.3961069598516519	0.0
30	0.0010972491962649637	0.0	0.0	0.666030262132833	0.0
31	0.0010972491962649637	0.0	0.0	1.43465332411644	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	270	0.0	30.14815	1
GCGGTAA	285	0.0	21.421053	23
ACTGAAA	90	9.368887E-8	20.555555	24
ATACGGC	45	0.0038136535	20.555555	29
CGTGCCA	310	0.0	20.290321	10
GGTTAAT	110	1.7262209E-9	20.181818	1
GTATCAA	405	0.0	20.098764	2
CGCGGTA	315	0.0	19.968254	22
ATCTAAG	75	9.195819E-6	19.733334	32
CATCTAA	75	9.195819E-6	19.733334	31
ATGAGGC	95	1.656881E-7	19.473684	6
TATACAC	95	1.656881E-7	19.473684	37
TAATGAG	95	1.656881E-7	19.473684	4
AACTCCG	315	0.0	19.380953	5
GGTAATA	320	0.0	19.078125	25
CCGTGCC	320	0.0	19.078125	9
AGCCGCG	330	0.0	19.060604	19
TCCGTGC	330	0.0	19.060604	8
AACGTCA	80	1.6045427E-5	18.5	28
TACTTAG	80	1.6045427E-5	18.5	2
>>END_MODULE