FastQCFastQC Report
Fri 10 Feb 2017
ERR1632708.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632708.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99384
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA2900.29179747243016985No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA2370.2384689688481043No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT1860.18715286162762618No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT1740.17507848345810192No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA1640.16501650165016502No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1510.15193592529984706No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC1490.1499235289382597No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA1490.1499235289382597No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA1360.1368429525879417No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC1310.13181196168397327No Hit
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT1300.13080576350317957TruSeq Adapter, Index 12 (95% over 22bp)
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT1200.12074378169524269No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC1190.119737583514449No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT1180.1187313853336553No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA1180.1187313853336553No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC1180.1187313853336553No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT1160.11671898897206794No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA1130.11370039442968687No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC1110.11168799806809948No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC1060.10665700716413105No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT1060.10665700716413105No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC1050.10565080898333737No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1450.033.1724131
CCTTGTA250.00547250529.62
ATACTGT358.812249E-426.42857236
CTGGTTC508.982433E-625.9000024
ACTGGTT551.8800794E-523.5454543
TGGTTCA400.001918862923.1255
GTCCACG400.001918862923.12519
GGATTAG400.001918862923.1251
GTATCAA2100.022.904762
TACTGTC652.6421221E-622.7692337
TACTGGT652.6421221E-622.769232
TTGTGTA502.6781025E-422.221
TTCTTCT855.0617018E-821.76470615
GTACTGG705.02496E-621.1428591
TATACAC806.8293957E-720.812536
ATACACA806.8293957E-720.812537
AGCCGGT909.2615664E-820.5555576
TTAGCCG909.2615664E-820.5555574
TAGCCGG909.2615664E-820.5555575
TCCAGGA450.003801625220.5555572
Produced by FastQC (version 0.11.5)